The calcium channel α<sub>2</sub>/δ<sub>1</sub> subunit interacts with ATP5b in the plasma membrane of developing muscle cells

Garcı́a, Jesús

doi:10.1152/ajpcell.00405.2010

Cited by 15 publications

(17 citation statements)

References 41 publications

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“…Thus, the fold increase in the levels of α 1, β , and γ were substantially higher than for α 2 δ 1. Overall the mRNA data demonstrate that α 2 δ 1 is expressed earlier than α 1 and at higher levels than β or γ subunits in satellite cells and are consistent with studies detecting different protein levels of a2 δ 1 and α 1 subunits early in development [9, 17]. …”

Section: Resultssupporting

confidence: 88%

“…The β subunit is an intracellular protein involved in the regulation of gene expression in different cell types including myoblasts [5–7]. The α 2 δ 1 protein is an extracellular protein involved in cell attachment and migration and possibly cell signaling in myoblasts [8, 9]. We recently found that the α 2 δ 1 subunit localizes at the leading ends of myotubes with little or no association with α 1 subunits 2 days after the induction of differentiation [8] suggesting that, in addition to attachment and migration, α 2 δ 1 may play a role in the elongation process of myotubes.…”

Section: Introductionmentioning

confidence: 99%

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Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage

Tamayo

Grajales

Garcı́a

2012

Journal of Signal Transduction

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Satellite cells can maintain or repair muscle because they possess stem cell properties, making them a valuable option for cell therapy. However, cell transplants into skeletal muscle of patients with muscular dystrophy are limited by donor cell attachment, migration, and survival in the host tissue. Cells used for therapy are selected based on specific markers present in the plasma membrane. Although many markers have been identified, there is a need to find a marker that is expressed at different states in satellite cells, activated, quiescent, or differentiated cell. Furthermore, the marker has to be present in human tissue. Recently we reported that the plasma membrane α2δ1 protein is involved in cell attachment and migration in myoblasts. The α2δ1 subunit forms a part of the L-type voltage-dependent calcium channel in adult skeletal muscle. We found that the α2δ1 subunit is expressed in the majority of newly isolated satellite cells and that it appears earlier than the α1 subunits and at higher levels than the β or γ subunits. We also found that those cells that expressed α2δ1 would differentiate into muscle cells. This evidence indicates that the α2δ1 may be used as a marker of satellite cells that will differentiate into muscle.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage

Tamayo

Grajales

Garcı́a

2012

Journal of Signal Transduction

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“…The presence of normal calcium currents in the BDNF mutant VMH suggests that anorexigenic effects of BDNF and ␣2␦-1 in this region are unlikely to be calcium channel dependent. Interestingly, effects of ␣2␦-1 unrelated to its role as a calcium channel subunit have been described in myotubes, where it is localized separately from calcium channel complexes (García, 2011). Knockdown of ␣2␦-1 in these cells impaired several cellular functions, including migration and attachment without affecting calcium currents (García et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, effects of ␣2␦-1 unrelated to its role as a calcium channel subunit have been described in myotubes, where it is localized separately from calcium channel complexes (García, 2011). Knockdown of ␣2␦-1 in these cells impaired several cellular functions, including migration and attachment without affecting calcium currents (García et al, 2008). Directly relevant to our findings, ␣2␦-1 also interacts with TSPs to facilitate excitatory, but not inhibitory, synapse assembly in retinal ganglion and cortical neurons (Eroglu et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Hypothalamic Dysfunction of the Thrombospondin Receptor α2δ-1 Underlies the Overeating and Obesity Triggered by Brain-Derived Neurotrophic Factor Deficiency

et al. 2014

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Brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, are critical components of the neural circuitry controlling appetite and body weight. Diminished BDNF signaling in mice results in severe hyperphagia and obesity. In humans, BDNF haploinsufficiency and the functional Bdnf Val66Met polymorphism have been linked to elevated food intake and body weight. The mechanisms underlying this dysfunction are poorly defined. We demonstrate a chief role of ␣2␦-1, a calcium channel subunit and thrombospondin receptor, in triggering overeating in mice with central BDNF depletion. We show reduced ␣2␦-1 cell-surface expression in the BDNF mutant ventromedial hypothalamus (VMH), an energy balance-regulating center. This deficit contributes to the hyperphagia exhibited by BDNF mutant mice because selective inhibition of ␣2␦-1 by gabapentin infusion into wild-type VMH significantly increases feeding and body weight gain. Importantly, viral-mediated ␣2␦-1 rescue in BDNF mutant VMH significantly mitigates their hyperphagia, obesity, and liver steatosis and normalizes deficits in glucose homeostasis. Whole-cell recordings in BDNF mutant VMH neurons revealed normal calcium currents but reduced frequency of EPSCs. These results suggest calcium channel-independent effects of ␣2␦-1 on feeding and implicate ␣2␦-1-thrombospondin interactions known to facilitate excitatory synapse assembly. Our findings identify a central mechanism mediating the inhibitory effects of BDNF on feeding. They also demonstrate a novel and critical role for ␣2␦-1 in appetite control and suggest a mechanism underlying weight gain in humans treated with gabapentinoid drugs.

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“…Indeed, ATP synthase subunit beta has been linked to Bcl-xl which is involved in apoptosis regulation (135). Subunit beta is also involved in calcium signalling at the plasma and intracellular membranes of developing muscle cells (136). As the main cytotoxic component of HAMLET is the oleic acid rather than the protein, the binding of HAMLET protein to ATP synthase protein subunits is likely a result of the unfolded HAMLET protein, no longer partially folded around its oleic acid cargo, binding to the ATP synthase subunits that are in proximity to HAMLET and are themselves now dissociated.…”

Section: Binding To Nucleotide-binding Proteinsmentioning

confidence: 99%

Structure and Potential Cellular Targets of HAMLET-like Anti-Cancer Compounds made from Milk Components

et al. 2015

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-The HAMLET family of compounds (Human Alpha-lactalbumin Made Lethal to Tumours) was discovered during studies on the properties of human milk, and is a class of protein-lipid complexes having broad spectrum anti-cancer, and some specific anti-bacterial properties. The structure of HAMLETlike compounds consists of an aggregation of partially unfolded protein making up the majority of the compound's mass, with fatty acid molecules bound in the hydrophobic core. This is a novel protein-lipid structure and has only recently been derived by small-angle X-ray scattering analysis. The structure is the basis of a novel cytotoxicity mechanism responsible for anti-cancer activity to all of the around 50 different cancer cell types for which the HAMLET family has been trialled. Multiple cytotoxic mechanisms have been hypothesised for the HAMLET-like compounds, but it is not yet clear which of those are the initiating cytotoxic mechanism(s) and which are subsequent activities triggered by the initiating mechanism(s). In addition to the studies into the structure of these compounds, this review presents the state of knowledge of the anti-cancer aspects of HAMLET-like compounds, the HAMLET-induced cytotoxic activities to cancer and non-cancer cells, and the several prospective cell membrane and intracellular targets of the HAMLET family. The emerging picture is that HAMLET-like compounds initiate their cytotoxic effects on what may be a cancer-specific target in the cell membrane that has yet to be identified.

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The calcium channel α₂/δ₁ subunit interacts with ATP5b in the plasma membrane of developing muscle cells

Abstract: García J. The calcium channel ␣2/␦1 subunit interacts with ATP5b in the plasma membrane of developing muscle cells.

Cited by 15 publications

References 41 publications

Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage

Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage

Hypothalamic Dysfunction of the Thrombospondin Receptor α2δ-1 Underlies the Overeating and Obesity Triggered by Brain-Derived Neurotrophic Factor Deficiency

Structure and Potential Cellular Targets of HAMLET-like Anti-Cancer Compounds made from Milk Components

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The calcium channel α2/δ1 subunit interacts with ATP5b in the plasma membrane of developing muscle cells

Abstract: García J. The calcium channel ␣2/␦1 subunit interacts with ATP5b in the plasma membrane of developing muscle cells.

Cited by 15 publications

References 41 publications

Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage

Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage

Hypothalamic Dysfunction of the Thrombospondin Receptor α2δ-1 Underlies the Overeating and Obesity Triggered by Brain-Derived Neurotrophic Factor Deficiency

Structure and Potential Cellular Targets of HAMLET-like Anti-Cancer Compounds made from Milk Components

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The calcium channel α₂/δ₁ subunit interacts with ATP5b in the plasma membrane of developing muscle cells