The Ca2+-activated, large conductance K+-channel (BKCa) is a player in the LH/hCG signaling cascade in testicular Leydig cells

Matzkin, María Eugenia; Lauf, S.; Spinnler, Katrin; Rossi, Soledad Paola; Köhn, F.-M.; Kunz, Lars; Calandra, Ricardo S.; Frungieri, Mónica Beatriz; Mayerhofer, Artur

doi:10.1016/j.mce.2012.12.015

Cited by 13 publications

(8 citation statements)

References 38 publications

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“…It has been reported that rat Sertoli cells express SLC7A1 which relies on the NA+-independent transport system to deliver arginine or other cationic amino acids to germ cells [47]. The KCNMB2 gene is a calcium-gated potassium channel that has been described in various mammalian endocrine cells, including human and hamster testicular Leydig cells [48]. It has been suggested that these channels contribute to Leydig cell steroidogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that these channels contribute to Leydig cell steroidogenesis. Interestingly, the presence of iberiotoxin, a specific channel blocker, did not induce changes in testosterone production by hamster Leydig cells in vitro under basal conditions, but a significant increase in testosterone levels was reported when hCG was added to culture media [48]. According to Gong et al .…”

Section: Discussionmentioning

confidence: 99%

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Seasonal differences in the testicular transcriptome profile of free-living European beavers (Castor fiber L.) determined by the RNA-Seq method

et al. 2017

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The European beaver (Castor fiber L.) is an important free-living rodent that inhabits Eurasian temperate forests. Beavers are often referred to as ecosystem engineers because they create or change existing habitats, enhance biodiversity and prepare the environment for diverse plant and animal species. Beavers are protected in most European Union countries, but their genomic background remains unknown. In this study, gene expression patterns in beaver testes and the variations in genetic expression in breeding and non-breeding seasons were determined by high-throughput transcriptome sequencing. Paired-end sequencing in the Illumina HiSeq 2000 sequencer produced a total of 373.06 million of high-quality reads. De novo assembly of contigs yielded 130,741 unigenes with an average length of 1,369.3 nt, N50 value of 1,734, and average GC content of 46.51%. A comprehensive analysis of the testicular transcriptome revealed more than 26,000 highly expressed unigenes which exhibited the highest homology with Rattus norvegicus and Ictidomys tridecemlineatus genomes. More than 8,000 highly expressed genes were found to be involved in fundamental biological processes, cellular components or molecular pathways. The study also revealed 42 genes whose regulation differed between breeding and non-breeding seasons. During the non-breeding period, the expression of 37 genes was up-regulated, and the expression of 5 genes was down-regulated relative to the breeding season. The identified genes encode molecules which are involved in signaling transduction, DNA repair, stress responses, inflammatory processes, metabolism and steroidogenesis. Our results pave the way for further research into season-dependent variations in beaver testes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Seasonal differences in the testicular transcriptome profile of free-living European beavers (Castor fiber L.) determined by the RNA-Seq method

et al. 2017

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“…BKCa channels were characterized to be activated by the increase of the intracellular Ca 2+ and to determine the cell membrane hyperpolarization. In Leydig cells, the hyperpolarization induced by the BKCa channels was speculated to activate a series of events that limits testosterone production [102]. Oppositely, hormones might regulate the intracellular Ca 2+ concentration in Leydig cells.…”

Section: Physiology and Alterations Of Calcium Signaling In Intersmentioning

confidence: 99%

Calcium Signaling in Interstitial Cells: Focus on Telocytes

Radu

Banciu

et al. 2017

IJMS

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In this review, we describe the current knowledge on calcium signaling pathways in interstitial cells with a special focus on interstitial cells of Cajal (ICCs), interstitial Cajal-like cells (ICLCs), and telocytes. In detail, we present the generation of Ca2+ oscillations, the inositol triphosphate (IP3)/Ca2+ signaling pathway and modulation exerted by cytokines and vasoactive agents on calcium signaling in interstitial cells. We discuss the physiology and alterations of calcium signaling in interstitial cells, and in particular in telocytes. We describe the physiological contribution of calcium signaling in interstitial cells to the pacemaking activity (e.g., intestinal, urinary, uterine or vascular pacemaking activity) and to the reproductive function. We also present the pathological contribution of calcium signaling in interstitial cells to the aortic valve calcification or intestinal inflammation. Moreover, we summarize the current knowledge of the role played by calcium signaling in telocytes in the uterine, cardiac and urinary physiology, and also in various pathologies, including immune response, uterine and cardiac pathologies.

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“…Leydig cells and germ cells from immature hamsters (ihaLCs and ihaGCs, respectively) were purified from a testicular cell suspension obtained through digestion with 0.2 mg/ml collagenase type I (Sigma-Aldrich), filtration using a 100 μm Nylon cell strainer and separation in a discontinuous Percoll density gradient as previously described [17]. ihaLCs and ihaGCs migrated to the 53% and 37% Percoll fractions, respectively.…”

Section: Purification Of Hamster Leydig Cells and Germ Cellsmentioning

confidence: 99%

Pleiotropic actions of melatonin in testicular peritubular myoid cells of immature Syrian hamsters

Riviere

Rossi

Tavalieri

et al. 2022

Biochimica et Biophysica Acta (BBA) - General Subjects

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The Ca2+-activated, large conductance K+-channel (BKCa) is a player in the LH/hCG signaling cascade in testicular Leydig cells

Cited by 13 publications

References 38 publications

Seasonal differences in the testicular transcriptome profile of free-living European beavers (Castor fiber L.) determined by the RNA-Seq method

Seasonal differences in the testicular transcriptome profile of free-living European beavers (Castor fiber L.) determined by the RNA-Seq method

Calcium Signaling in Interstitial Cells: Focus on Telocytes

Pleiotropic actions of melatonin in testicular peritubular myoid cells of immature Syrian hamsters

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