The C-terminal domain of the Vibrio fischeri transcription activator LuxR is not essential for degradation by Lon protease

Melkina, O. E.; Манухов, И. В.; Завильгельский, Г Б

doi:10.1134/s0026893310030143

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…In our previous study, we showed that the NTD of LuxR is targeted by Lon protease (18). Taken together, these data indicate that the NTD of LuxR may play a role both in quorum sensing and in the recognition of the stress signals.…”

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confidence: 57%

The N-Terminal Domain of Aliivibrio fischeri LuxR Is a Target of the GroEL Chaperonin

et al. 2010

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confidence: 57%

The N-Terminal Domain of Aliivibrio fischeri LuxR Is a Target of the GroEL Chaperonin

et al. 2010

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“…The increase in the background luminosity ( Figure 2 and Figure 3 ) could be explained by a partial degradation of LuxR proteins by proteases with a subsequent AI-independent activation of transcription by their C-terminal fragments. The AI-independent activation of luxCDABE and luxI promoters by the C-terminal domain of LuxR was shown in [ 28 , 32 , 33 ]. The luxR1 protein is not subject to degradation by the Lon protease and does not require GroEL/ES for assembly [ 22 ].…”

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confidence: 99%

Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone

et al. 2021

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Aliivibrio fischeri LuxR and Aliivibrio logei LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of luxICDABEG genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the Aliivibrio cells, luxR genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the luxR expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection. Escherichia coli-based bacterial lux-biosensors were used, in which Photorhabdus luminescensluxCDABE genes were controlled by LuxR-dependent promoters and luxR, luxR1, or luxR2 regulatory genes. We varied either the dosage of the regulatory gene in the cells using additional plasmids, or the level of the regulatory gene expression using the lactose operon promoter. It was shown that an increase in expression level, as well as dosage of the regulatory gene in biosensor cells, leads to an increase in sensitivity (the threshold concentration of AI is reduced by one order of magnitude) and to a two to threefold reduction in response time. The best parameters were obtained for a biosensor with an increased dosage of luxRA. fischeri (sensitivity to 3-oxo-hexanoyl-l-homoserine lactone reached 30–100 pM).

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Real-time measurement of quorum-sensing signal autoinducer 3OC6HSL by a FRET-based nanosensor

Zhang

2013

Bioprocess Biosyst Eng

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Quorum sensing (QS) is involved in many important biological functions such as luminescence, antibiotic production, and biofilm formation. The autoinducer N-(3-oxo-hexanoyl)-L-homoserine lactone (3OC6HSL) plays a significant role in the QS system of the marine bacterium Vibrio fischeri. Tracing 3OC6HSL would be significant in studies related to QS signal transduction. Traditional detection of QS signaling molecules has relied on bacterial reporter strains and high-performance liquid chromatography, which are time consuming and have low sensitivity. Because 3OC6HSL binding to LuxR from V. fischeri causes a conformational change, we developed a genetically encoded biosensor based on Förster resonance energy transfer (FRET) by inserting LuxR between the FRET pair YFP/CFP. The detection limit of the sensor was 100 μM. We attained an optimized sensor with 70 % Δratio increase by screening different hydrophobic linkers, and demonstrated the feasibility of this sensor for visualizing 3OC6HSL both in vitro and in vivo.

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The C-terminal domain of the Vibrio fischeri transcription activator LuxR is not essential for degradation by Lon protease

Cited by 6 publications

References 14 publications

The N-Terminal Domain of Aliivibrio fischeri LuxR Is a Target of the GroEL Chaperonin

The N-Terminal Domain of Aliivibrio fischeri LuxR Is a Target of the GroEL Chaperonin

Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone

Real-time measurement of quorum-sensing signal autoinducer 3OC6HSL by a FRET-based nanosensor

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