The c-Fos and c-Jun from Litopenaeus vannamei play opposite roles in Vibrio parahaemolyticus and white spot syndrome virus infection

Li, Chaozheng; Li, Haoyang; Wang, Sheng; Song, Xuan; Zhang, Zijian; Zhang, Qian; Zuo, Hongliang; Xu, Xiaopeng; Weng, Shaoping; He, Jianguo

doi:10.1016/j.dci.2015.04.009

Cited by 51 publications

(21 citation statements)

References 36 publications

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“…This hypothesis is supported by the observation that JNK-phosphorylation levels are evidently reduced by JNK-inhibitor (SP600125) treatment in vivo (120, 121). More direct evidence should be demonstrated to further explore whether JNK can interact with and phosphorylate c-Jun in vitro (119). Next, c-Fos, another member of the AP-1 family, has been shown to form a heterodimer with c-Jun, which is also able to form a homodimer with itself, suggesting that c-Fos might also be downstream substrate of JNK (119).…”

Section: The Imd Signaling Pathway In Shrimpmentioning

confidence: 99%

“…More direct evidence should be demonstrated to further explore whether JNK can interact with and phosphorylate c-Jun in vitro (119). Next, c-Fos, another member of the AP-1 family, has been shown to form a heterodimer with c-Jun, which is also able to form a homodimer with itself, suggesting that c-Fos might also be downstream substrate of JNK (119). The ATF2, a member of the ATF/cAMP response-element-binding family of transcription factors, contains a common feature of the bZIP element, which is able to form homodimers or heterodimers with other proteins that contain bZIP elements, such as the AP-1 (131).…”

Section: The Imd Signaling Pathway In Shrimpmentioning

confidence: 99%

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The Two NF-κB Pathways Regulating Bacterial and WSSV Infection of Shrimp

Wang

2019

Front. Immunol.

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138

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The outbreak of diseases ordinarily results from the disruption of the balance and harmony between hosts and pathogens. Devoid of adaptive immunity, shrimp rely largely on the innate immune system to protect themselves from pathogenic infection. Two nuclear factor-κB (NF-κB) pathways, the Toll and immune deficiency (IMD) pathways, are generally regarded as the major regulators of the immune response in shrimp, which have been extensively studied over the years. Bacterial infection can be recognized by Toll and IMD pathways, which activate two NF-κB transcription factors, Dorsal and Relish, respectively, to eventually lead to boosting the expression of various antimicrobial peptides (AMPs). In response to white-spot-syndrome-virus (WSSV) infection, these two pathways appear to be subverted and hijacked to favor viral survival. In this review, the recent progress in elucidating microbial recognition, signal transduction, and effector regulation within both shrimp Toll and IMD pathways will be discussed. We will also highlight and discuss the similarities and differences between shrimps and their Drosophila or mammalian counterparts. Understanding the interplay between pathogens and shrimp NF-κB pathways may provide new opportunities for disease-prevention strategies in the future.

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Section: The Imd Signaling Pathway In Shrimpmentioning

confidence: 99%

Section: The Imd Signaling Pathway In Shrimpmentioning

confidence: 99%

The Two NF-κB Pathways Regulating Bacterial and WSSV Infection of Shrimp

Wang

2019

Front. Immunol.

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138

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“…Based on SP approach, we identified a specific gene FOS . It is also a tumor-associated gene, which encodes a leucine zipper protein that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1 [ 49 ]. Related to crucial pathways such as NF-kB and MAPK, FOS is quite significant in inflammation initiation, especially in the digestive tract [ 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

Identification of Candidate Genes Related to Inflammatory Bowel Disease Using Minimum Redundancy Maximum Relevance, Incremental Feature Selection, and the Shortest-Path Approach

Yuan

Zhang

Kong

et al. 2017

BioMed Research International

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Identification of disease genes is a hot topic in biomedicine and genomics. However, it is a challenging problem because of the complexity of diseases. Inflammatory bowel disease (IBD) is an idiopathic disease caused by a dysregulated immune response to host intestinal microflora. It has been proven to be associated with the development of intestinal malignancies. Although the specific pathological characteristics and genetic background of IBD have been partially revealed, it is still an overdetermined disease and the blueprint of all genetic variants still needs to be improved. In this study, a novel computational method was built to identify genes related to IBD. Samples from two subtypes of IBD (ulcerative colitis and Crohn's disease) and normal samples were employed. By analyzing the gene expression profiles of these samples using minimum redundancy maximum relevance and incremental feature selection, 21 genes were obtained that could effectively distinguish samples from the two subtypes of IBD and the normal samples. Then, the shortest-path approach was used to search for an additional 20 genes in a large network constructed using protein-protein interactions based on the above-mentioned 21 genes. Analyses of the 41 genes obtained indicate that they are closely associated with this disease.

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“…Muscle DNA was extracted with TIANGEN Marine Animals DNA Kit (TIANGEN, China) according to the user’s manual. The quantities of WSSV genome copies were measured by utilizing absolute quantitative PCR with primers WSSV32678-F/WSSV32753-R and a TaqMan fluorogenic probe as described previously (49). The survival rate of each group was recorded every 4 h. The Mantel-Cox (log-rank χ 2 test) method was subjected to analyze differences between groups with the GraphPad Prism software.…”

Section: Methodsmentioning

confidence: 99%

Penaeidins are a novel family of antiviral effectors against WSSV in shrimp

Xiao

Niu

et al. 2018

Preprint

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33Penaeidins are members of a family of key effectors with broad anti-bacterial 34 activities in penaeid shrimp. However, the function of penaeidins in antiviral 35 immunity is rarely reported and remains largely unknown. Herein, we uncovered that 36 penaeidins are a novel family of antiviral effectors against white spot syndrome virus 37 (WSSV). Firstly, RNAi in vivo mediated knockdown of each penaeidin from four 38 identified penaeidins from Litopenaeus vannamei resulted in elevated viral loads and 39 rendered shrimp more susceptible to WSSV, whilst the phenotype of survival rate in 40 penaeidin-silenced shrimp can be rescued via the injection of recombinant penaeidin 41 proteins. Moreover, pull-down assays demonstrated the conserved PEN domain of 42 penaeidin was able to interact with WSSV structural proteins. Furthermore, we 43 observed that colloidal gold-labeled penaeidins were located on the outer surface of 44 the WSSV virion. By infection-blocking assay, we observed that hemocytes had lower 45 viral infection rates in the group of WSSV preincubated with penaeidins than those of 46 control group. Phagocytic activity analysis further showed that penaeidins were able 47 to inhibit phagocytic activity of hemocytes against WSSV. Taken together, these 48 results suggest that penaeidins specifically binds to WSSV virion by interacting with 49 its structural proteins, thus preventing viral infection that confers host against WSSV. 50In addition, dual-luciferase assay and EMSA assay demonstrated that penaeidins were 51 regulated by Dorsal and Relish, two transcription factors of the canonical Toll and 52 IMD pathway, respectively. To our best knowledge, this is the first report on 53 uncovering the antiviral function of penaeidins in the innate immune system of 54 shrimp. 55 56

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The c-Fos and c-Jun from Litopenaeus vannamei play opposite roles in Vibrio parahaemolyticus and white spot syndrome virus infection

Cited by 51 publications

References 36 publications

The Two NF-κB Pathways Regulating Bacterial and WSSV Infection of Shrimp

The Two NF-κB Pathways Regulating Bacterial and WSSV Infection of Shrimp

Identification of Candidate Genes Related to Inflammatory Bowel Disease Using Minimum Redundancy Maximum Relevance, Incremental Feature Selection, and the Shortest-Path Approach

Penaeidins are a novel family of antiviral effectors against WSSV in shrimp

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