The C. elegans Taste Receptor Homolog LITE-1 Is a Photoreceptor

Gong, Jianke; Yuan, Yiyuan; Ward, Andrew; Kang, Lijun; Zhang, Bi; Wu, Zhiping; Peng, Junmin; Feng, Zhaoyang; Liu, Jianfeng; Xu, Xiaoxiao

doi:10.1016/j.cell.2016.12.040

Cited by 27 publications

(28 citation statements)

References 12 publications

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“…We used the microfluidic device to deliver taste and odor cues that are perceived by the worm in a lateralized manner: a) a repulsively high concentration of the gustatory cue NaCl, previously shown to trigger an ON response in the left ASE and an OFF response in the right ASE (Suzuki et al, 2008), b) the attractive odorant 2-butanone, which is sensed by AWC-ON, stochastically chosen in development as either the left or right AWC neuron (Wes and Bargmann, 2001) and, c) the attractive odorant 2,3pentanedione which evokes the opposite lateralized response in AWC-OFF (the AWC neuron contralaterally opposed to the one sensing 2-butanone) (Wes and Bargmann, 2001). We also delivered repulsive blue light (via laser), previously shown to evoke its avoidance response through LITE-1, a photoreceptor protein (Edwards et al, 2008;Gong et al, 2017). Hence, these four stimuli provided an opportunity to measure circuit responses for three sensory modalities, representing both attractive and repulsive valences, while exploring the downstream representations of asymmetric sensory inputs.…”

Section: Using Neuropal For Whole-brain Calcium Imaging Of Stimulus Rmentioning

confidence: 99%

NeuroPAL: A Neuronal Polychromatic Atlas of Landmarks for Whole-Brain Imaging inC. elegans

Yemini

Lin

Nejatbakhsh

et al. 2019

Preprint

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ABSTRACTComprehensively resolving single neurons and their cellular identities from whole-brain fluorescent images is a major challenge. We achieve this in C. elegans through the engineering and use of a multicolor transgene called NeuroPAL (a Neuronal Polychromatic Atlas of Landmarks). NeuroPAL worms share a stereotypical multicolor fluorescence map for the entire hermaphrodite nervous system that allows comprehensive determination of neuronal identities. Neurons labeled with NeuroPAL do not exhibit fluorescence in the green, cyan, or yellow emission channels, allowing the transgene to be used with numerous reporters of gene expression or neuronal dynamics. Here we showcase three studies that leverage NeuroPAL for nervous-system-wide neuronal identification. First, we determine the brainwide expression patterns of all metabotropic receptors for acetylcholine, GABA, and glutamate, completing a map of this communication network. Second, we uncover novel changes in cell fate caused by transcription factor mutations. Third, we record brainwide activity in response to attractive and repulsive chemosensory cues, characterizing multimodal coding and novel neuronal asymmetries for these stimuli. We present a software package that enables semi-automated determination of all neuronal identities based on color and positional information. The NeuroPAL framework and software provide a means to design landmark atlases for other tissues and organisms. In conclusion, we expect NeuroPAL to serve as an invaluable tool for gene expression analysis, neuronal fate studies, and for mapping whole-brain activity patterns.

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Section: Using Neuropal For Whole-brain Calcium Imaging Of Stimulus Rmentioning

confidence: 99%

NeuroPAL: A Neuronal Polychromatic Atlas of Landmarks for Whole-Brain Imaging inC. elegans

Yemini

Lin

Nejatbakhsh

et al. 2019

Preprint

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“…Our proposed model adds to the increasing evidence for ROS acting as second messengers and for light-induced ROS mediating various signaling pathways. [26][27][28][29][30] Our findings propose a novel role for mitochondria in UVR-induced skin pigmentation. Mitochondria are vital for cellular homeostasis, as they regulate cellular energy, metabolism, survival, and apoptosis.…”

Section: Discussionmentioning

confidence: 70%

Cross talk between calcium and ROS regulate the UVA‐induced melanin response in human melanocytes

2020

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Exposure to high doses of solar long wavelength ultraviolet radiation (UVA) damages human skin via reactive oxygen species (ROS). Whether physiological UVA doses also generate ROS that has an effect on the skin remains unknown. We previously showed that in human epidermal melanocytes UVA activates a G‐protein coupled signaling pathway that leads to calcium mobilization and increased melanin. Here, we report that ROS generated by the UVA phototransduction pathway are critical cellular messengers required to augment melanin. Using simultaneous UVA exposure and live‐cell imaging of primary human melanocytes, we found that physiological doses of UVA generate two spatiotemporally distinct sources of ROS: one upstream of the G‐protein activation that potentiates calcium responses, and another source downstream of calcium, in the mitochondria (ROSmito). UVA‐evoked signaling led to mitochondrial calcium uptake via mitochondrial calcium uniporter to promote ROSmito production leading to melanin synthesis. Our findings reveal a novel mechanism in which ROS function as signaling messengers necessary for melanin production, thus having a protective role in the UVA‐induced skin response.

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“…While the response appeared intact in the GUR-3 null strain, it was severely deficient in the two strains lacking functional LITE-1. LITE-1 and GUR-3 share 40% homology and are both involved in the inhibition of feeding response to UV light Gong et al 2016); however, only LITE-1 is thought to participate in the avoidance response, as the response appears intact in GUR-3 mutants . Therefore, our finding that X-ray avoidance also appears intact in the strain with mutated GUR-3, but functional LITE-1, is in line with previous findings demonstrating a prominent role for LITE-1, but not GUR-3, in locomotory avoidance behavior.…”

Section: Discussionmentioning

confidence: 99%

LITE-1 Dependent X-Ray Sensitivity in C. elegans

Cannon

Ranasinghe

et al. 2019

Preprint

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We report the finding that C. elegans display X-ray avoidance behavior at high but well tolerated doses, and that this behavior appears to require LITE-1, a gustatory receptor that has been implicated in UV avoidance behavior. We recorded acute behavioral responses of wild-type worms to increasing intensities of Xray stimulation and found a positive stimulation-response relationship. Mutant strains of worms with dysfunctional photoreceptor proteins LITE-1 and GUR-3 were assayed, and the X-ray avoidance response was found to be nearly absent in LITE-1 mutants but not GUR-3 mutants, suggesting a prominent role for LITE-1 in the detection of X-rays. These findings may be important for developing optogenetics tools to stimulate cells in deep tissue using X-rays, for understanding the mechanism of LITE-1 signaling, and for understanding how organisms may respond to radiation. C. elegans | x-ray | optogenetics | LITE-1

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The C. elegans Taste Receptor Homolog LITE-1 Is a Photoreceptor

Cited by 27 publications

References 12 publications

NeuroPAL: A Neuronal Polychromatic Atlas of Landmarks for Whole-Brain Imaging inC. elegans

NeuroPAL: A Neuronal Polychromatic Atlas of Landmarks for Whole-Brain Imaging inC. elegans

Cross talk between calcium and ROS regulate the UVA‐induced melanin response in human melanocytes

LITE-1 Dependent X-Ray Sensitivity in C. elegans

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