The bulk of the hematopoietic stem cell population is dispensable for murine steady-state and stress hematopoiesis

Schoedel, Kristina B.; Morcos, Mina N.F.; Zerjatke, Thomas; Roeder, Ingo; Grinenko, Tatyana; Voehringer, David; Göthert, Joachim R; Waskow, Claudia; Roers, Axel; Gerbaulet, Alexander

doi:10.1016/j.exphem.2016.06.209

Cited by 7 publications

(16 citation statements)

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“…We speculate that discrepancies between recent studies reporting high (Sawai et al., 2016) or low (Busch et al., 2015, Schoedel et al., 2016, Sun et al., 2014) contribution of HSCs to steady-state hematopoiesis might reflect lineage tracing of exclusive HSC subpopulations that differ by SCA-1 expression and cell-cycle activity.…”

Section: Discussionmentioning

confidence: 99%

SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells

et al. 2017

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SummaryBlood cell generation depends on continuous cellular output by the sequential hierarchy of hematopoietic stem cell (HSC) and progenitor populations that all contain quiescent and actively cycling cells. Hematopoietic stem and progenitor cells (HSPCs) express the surface molecule Stem cell antigen 1 (SCA-1/LY6A). Using histone 2B-red fluorescent fusion protein label retention and cell-cycle reporter mice, we demonstrate that high SCA-1 expression (SCA-1hi) identifies not only quiescent HSCs but quiescent cells on all hierarchical levels within the lineage−SCA-1+KIT+ (LSK) population. Each transplanted SCA-1hi HSPC population also displayed self-renewal potential superior to that of the respective SCA-1lo population. SCA-1 expression is inducible by type I interferon (IFN). We show, however, that quiescence and high self-renewal capacity of cells with brighter SCA-1 expression at steady state were independent of type I IFN signaling. We conclude that SCA-1 expression levels can be used to prospectively isolate functionally heterogeneous HSPC subpopulations.

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Section: Discussionmentioning

confidence: 99%

SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells

et al. 2017

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“…18 Consistent with these findings, ablation of .90% of HSCs had minimal impact on steady-state hematopoiesis. 19 By contrast, a recent lineage-tracing study using Pdzk1ip1-CreER strain reported extensive contribution of labeled HSCs to hematopoiesis in steady state. 20 Furthermore, labeling HSC clones with multiple fluorescent proteins in HUe mice revealed that native hematopoiesis is supported by several large clones that are relatively stable, although some clones fluctuated in terms of their size.…”

Section: Introductionmentioning

confidence: 89%

Lineage tracing of murine adult hematopoietic stem cells reveals active contribution to steady-state hematopoiesis

Chapple¹,

Tseng

Hu³

et al. 2018

Blood Advances

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“…The latter was surprising, as steady-state type I IFN partially accounted for SCA-1 expression ( Figure S2G) and ''tonic'' (basal or constitutive) type I IFN signaling has been implied in HSC maintenance (Gough et al, 2012 Hematopoietic contribution, differentiation pattern, and the relationship between the HSPC subpopulations and diverse SCA-1 expression have not been addressed in situ so far. We speculate that discrepancies between recent studies reporting high (Sawai et al, 2016) or low (Busch et al, 2015;Schoedel et al, 2016;Sun et al, 2014) contribution of HSCs to steady-state hematopoiesis might reflect lineage tracing of exclusive HSC subpopulations that differ by SCA-1 expression and cell-cycle activity.…”

Section: Discussionmentioning

confidence: 68%

Sca-1 expression level identifies quiescent hematopoietic stem and progenitor cells

Morcos

Schoedel

Hoppe

et al. 2017

Experimental Hematology

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SUMMARYBlood cell generation depends on continuous cellular output by the sequential hierarchy of hematopoietic stem cell (HSC) and progenitor populations that all contain quiescent and actively cycling cells. Hematopoietic stem and progenitor cells (HSPCs) express the surface molecule Stem cell antigen 1 (SCA-1/LY6A). Using histone 2B-red fluorescent fusion protein label retention and cell-cycle reporter mice, we demonstrate that high SCA-1 expression (SCA-1 hi ) identifies not only quiescent HSCs but quiescent cells on all hierarchical levels within the lineage À SCA-1 + KIT + (LSK) population. Each transplanted SCA-1 hi HSPC population also displayed self-renewal potential superior to that of the respective SCA-1 lo population. SCA-1 expression is inducible by type I interferon (IFN). We show, however, that quiescence and high self-renewal capacity of cells with brighter SCA-1 expression at steady state were independent of type I IFN signaling. We conclude that SCA-1 expression levels can be used to prospectively isolate functionally heterogeneous HSPC subpopulations.

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The bulk of the hematopoietic stem cell population is dispensable for murine steady-state and stress hematopoiesis

Cited by 7 publications

References 0 publications

SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells

SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells

Lineage tracing of murine adult hematopoietic stem cells reveals active contribution to steady-state hematopoiesis

Sca-1 expression level identifies quiescent hematopoietic stem and progenitor cells

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