The budding yeast endocytic pathway

Toret, Christopher P.; Drubin, David G.

doi:10.1242/jcs.03251

Cited by 80 publications

(71 citation statements)

References 20 publications

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“…The decrease in cytostome number suggests that actin may play a role early in cytostome formation, as no change or an increase in cytostome number would be expected if actin impacted only late stages of cytostome development. Actin may help to recruit protein complexes required for initial perturbation of the PVM/PPM, which aids in the formation of the cytostome invagination, as has been proposed for endocytosis in other systems (Qualmann et al, 2000;Toret and Drubin, 2006;Yarar et al, 2005). Although P. falciparum encodes components of the clathrin-coat complex, we did not observe morphological evidence of clathrin association with cytostomes.…”

Section: The Role(s) Of Actin In Cytostome Structure and Regulationsupporting

confidence: 47%

A new model for hemoglobin ingestion and transport by the human malaria parasite Plasmodium falciparum

Lazarus

Schneider

Taraschi

2008

Journal of Cell Science

108

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The current model for hemoglobin ingestion and transport by intraerythrocytic Plasmodium falciparum malaria parasites shares similarities with endocytosis. However, the model is largely hypothetical, and the mechanisms responsible for the ingestion and transport of host cell hemoglobin to the lysosome-like food vacuole (FV) of the parasite are poorly understood. Because actin dynamics play key roles in vesicle formation and transport in endocytosis, we used the actin-perturbing agents jasplakinolide and cytochalasin D to investigate the role of parasite actin in hemoglobin ingestion and transport to the FV. In addition, we tested the current hemoglobin trafficking model through extensive analysis of serial thin sections of parasitized erythrocytes (PE) by electron microscopy. We find that actin dynamics play multiple, important roles in the hemoglobin transport pathway, and that hemoglobin delivery to the FV via the cytostomes might be required for parasite survival. Evidence is provided for a new model, in which hemoglobin transport to the FV occurs by a vesicle-independent process.

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Section: The Role(s) Of Actin In Cytostome Structure and Regulationsupporting

confidence: 47%

A new model for hemoglobin ingestion and transport by the human malaria parasite Plasmodium falciparum

Lazarus

Schneider

Taraschi

2008

Journal of Cell Science

108

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“…Moreover, it has been previously reported that the early steps of endocytosis are mediated by actin polymerization in yeast [47][48][49][50][51][52] . Actin polymerization comprises recruitment and interplay of different actin related proteins at the site of internalization.…”

Section: Polymerization Are Required For Delivery Of Cargo Proteins Tmentioning

confidence: 99%

“…Subsequently, Myo3p and Myo5p are mobilized during the later stages of actin polymerization. Finally, Rvs161p and Rvs167p facilitate scission of endocytic vesicles [47][48][49][50][51][52] . Initially, the distribution of FBPase was studied in a yeast strain where the END3 gene had been deleted [46] .…”

Section: Polymerization Are Required For Delivery Of Cargo Proteins Tmentioning

confidence: 99%

Vacuole import and degradation pathway: Insights into a specialized autophagy pathway

Alibhoy¹

2011

WJBC

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Glucose deprivation induces the synthesis of pivotal gluconeogenic enzymes such as fructose-1,6-bisphosphatase, malate dehydrogenase, phosphoenolpyruvate carboxykinase and isocitrate lyase in Saccharomyces cerevisiae . However, following glucose replenishment, these gluconeogenic enzymes are inactivated and degraded. Studies have characterized the mechanisms by which these enzymes are inactivated in response to glucose. The site of degradation of these proteins has also been ascertained to be dependent on the duration of starvation. Glucose replenishment of short-term starved cells results in these proteins being degraded in the proteasome. In contrast, addition of glucose to cells starved for a prolonged period results in these proteins being degraded in the vacuole. In the vacuole dependent pathway, these proteins are sequestered in specialized vesicles termed vacuole import and degradation (Vid). These vesicles converge with the endocytic pathway and deliver their cargo to the vacuole for degradation. Recent studies have identified that internalization, as mediated by actin polymerization, is essential for delivery of cargo proteins to the vacuole for degradation. In addition, components of the target of rapamycin complex 1 interact with cargo proteins during glucose starvation. Furthermore, Tor1p dissociates from cargo proteins following glucose replenishment. Future studies will be needed to elaborate on the importance of internalization at the plasma membrane and the subsequent import of cargo proteins into Vid vesicles in the vacuole dependent degradation pathway.

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“…These kinases, again, are partially redundant, but many results point to both shared and divergent roles of these kinases in budding yeast morphogenesis. Ste20 and Cla4 have been shown to phosphorylate and activate type I myosins Myo3 and Myo5 [98], which in turn have a role in the internalization step of endocytosis via actin patches [99,100]. Cla4 is also the major kinase that phosphorylates the GEF Cdc24, although the exact role for this phosphorylation is still controversial [74,101].…”

Section: Regulatory Linkages Downstream Of Cdc42mentioning

confidence: 99%

Yeast and fungal morphogenesis from an evolutionary perspective

Wedlich‐Söldner

2008

Seminars in Cell & Developmental Biology

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Cellular morphogenesis is a complex process and molecular studies in the last few decades have amassed a large amount of information that is difficult to grasp in any completeness. Fungal systems, in particular the budding and fission yeasts, have been important players in unravelling the basic structural and regulatory elements involved in a wide array of cellular processes. In this article, we address the design principles underlying the various processes of yeast and fungal morphogenesis. We attempt to explain the apparent molecular complexity from the perspective of the evolutionary theory of "facilitated variation". Following a summary of some of the most studied morphogenetic phenomena, we discuss, using recent examples, the underlying core processes and their associated "weak" regulatory linkages that bring about variation in morphogenetic phenotypes.

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The budding yeast endocytic pathway

Abstract: There was an error published in J. Cell Sci. 119, 4585-4587.We apologise for an error in the poster accompanying this article. The WASP/Myo module was omitted in Step 7 of the budding yeast endocytic pathway.Below, Step 7 is shown correctly.

Cited by 80 publications

References 20 publications

A new model for hemoglobin ingestion and transport by the human malaria parasite Plasmodium falciparum

A new model for hemoglobin ingestion and transport by the human malaria parasite Plasmodium falciparum

Vacuole import and degradation pathway: Insights into a specialized autophagy pathway

Yeast and fungal morphogenesis from an evolutionary perspective

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