The BTB domain, found primarily in zinc finger proteins, defines an evolutionarily conserved family that includes several developmentally regulated genes in Drosophila.

Abstract

The Drosophila bric A brac protein and the transcriptional regulators encoded by tramt and BroadComplex contain a highly conserved domain of =115 amino acids, which we have cafled the BTB domain. We have identifed six additonal Drosophila genes that encode this domain. Five of these genes are developmentally regulated, and one of them appears to be functionally related to bric a brac. The BTB domain defines a gene family with an estimated 40 members in Drosophila. This domain is found primarily at the N terminus of zinc finger proteins and is evolutionarily conserved from Drosophia to mammals.

Expression pattern of Gal4 enhancer trap insertions into the bric à brac locus generated by P element replacement

“…bab function is not required for embryogenesis; however, bab2 (previously called BTB2) is expressed in the embryo (Zollman et al, 1994). During embryogenesis, both bab and bab Pgal4-2 express Gal4 in the posterior spiracles (Fig.…”

Distinct roles of Broad isoforms in regulation of the 20-hydroxyecdysone effector gene, Vitellogenin, in the mosquito Aedes aegypti

“…In Drosophila, four BR isoforms (Z1-Z4) share a common N-terminal Bric-a-brac-Tramtrack-Broad (BTB) domain, an evolutionarily conserved protein-protein interaction module (Zollman et al, 1994). Alternative splicing results in isoform-specific C-termini containing one of four pairs of C2H2 zinc fingers that recognize different DNA sequences (DiBello et al, 1991;Bayer et al, 1996).…”

BCL-6 is phosphorylated at multiple sites in its serine- and proline-clustered region by mitogen-activated protein kinase (MAPK) in vivo

“…The BCL-6 gene encodes a protein containing a DNA binding domain consisting of six C-terminal zinc®nger motifs Baron et al, 1995), and an N-terminal BTB/POZ domain, which was found to act as a protein-protein interaction domain (Bardwell and Treisman, 1994;Zollman et al, 1994). Conserved N-terminal BTB/POZ and Cterminal zinc-®nger domains have also been identi®ed in some transcription factors, such as the Drosphila tramtrack (ttk) protein (Harrison and Travers, 1990), Broad-complex (Br-c) protein (DiBello et al, 1991), human KUP protein (Chardin et al, 1991) and ZID protein (Bardwell et al, 1994), suggesting that BCL-6 acts as a transcription factor.…”