“…NHEJ are used to study gene function and for trait development even for polyploidy plants (Curtin et al, 2013;Qi et al, 2013). Gene knockout using SSNs has been used to elucidate plant gene of important genes that could be used for crop improvement in diverse plant species, including several important crops, such as barley, soybean, maize, rice, wheat, and sorghum (Voytas and Gao, 2014;Curtin et al, 2013;Qi et al, 2013;Wendt et al, 2013;Gao et al, 2010;Lyznik et al, 1991;Yang et al, 2009;Shukla et al, 2009;Liang et al, 2013;Jiang et al, 2013;Wang et al, 2013;.…”