The Brassica napus Calcineurin B-Like 1/CBL-interacting protein kinase 6 (CBL1/CIPK6) component is involved in the plant response to abiotic stress and ABA signalling

Abstract

A CBL-interacting protein kinase (CIPK) gene, BnCIPK6, was isolated in Brassica napus. Through yeast two-hybrid screening, 27 interaction partners (including BnCBL1) of BnCIPK6 were identified in Brassica napus. Interaction of BnCIPK6 and BnCBL1 was further confirmed by BiFC (bimolecular fluorescence complementation) in plant cells. Expressions of BnCIPK6 and BnCBL1 were significantly up-regulated by salt and osmotic stresses, phosphorous starvation, and abscisic acid (ABA). Furthermore, BnCIPK6 promoter activity was intensively induced in cotyledons and roots under NaCl, mannitol, and ABA treatments. Transgenic Arabidopsis plants with over-expressing BnCIPK6, its activated form BnCIPK6M, and BnCBL1 enhanced high salinity and low phosphate tolerance, suggesting that the functional interaction of BnCBL1 and BnCIPK6 may be important for the high salinity and phosphorous deficiency signalling pathways. In addition, activation of BnCIPK6 confers Arabidopsis plants hypersensitive to ABA. On the other hand, over-expression of BnCIPK6 in Arabidopsis cipk6 mutant completely rescued the low-phosphate-sensitive and ABA-insensitive phenotypes of this mutant, further suggesting that BnCIPK6 is involved in the plant response to high-salinity, phosphorous deficiency, and ABA signalling.

Characterization of Salinity Tolerance of Transgenic Rice Lines Harboring HsCBL8 of Wild Barley (Hordeum spontanum) Line from Qinghai-Tibet Plateau

“…By contrast, double- cbl2/cbl3 -mutant plants were found more resistant to high Na + and low K + conditions (Tang et al, 2012). Similar stress responses of CBLs have also been reported in rice OsCBL8 (Xiang et al, 2007), Ammopiptanthus mongolicus AmCBL1 (Chen et al, 2011), soybean GmCBL1 (Li et al, 2012), rape BnCBL1 (Chen et al, 2012), and tobacco NsylCBL10 (Dong et al, 2015). Ten CBL proteins have also been discovered in Arabidopsis , rice and populous (Albrecht et al, 2001; Kolukisaoglu et al, 2004; Zhang et al, 2008).…”

Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat

“…MPK genes were reported to participate in response to cold, drought, ultraviolet light, oxidation stress and disease in many crops [45–47]. Thirdly, CBL7 , as one of the plant calcium sensors, can interact with CIPKs to form CBL-CIPK complexes that mediate responses to salinity, drought stress, phosphorous deficiency and ABA signaling [48–50]. Li et al [49] indicated that over-expression of soybean CBL1 enhances tolerance to salinity and drought stress in Arabidopsis .…”

Selection of reference genes for quantitative real-time PCR in Cocos nucifera during abiotic stress

“…In addition to the top-ranked classic HKGs such as eEF1-␣ and UBC10, the actin and rRNA gene are also used for normalizing stress-responsive genes in a wide range of species, including soybean, rice, and Brassica napus L. (Chen et al 2012;Nakashima et al 2007;Novillo et al 2012;Yan et al 2011;Zhou et al 2010). However, normalization by the less stably expressed 25S rRNA showed distinct differences in expression results for the target CBF-like gene compared with normalization using the eEF1-␣ and UBC10 genes, with low expression levels and weaker variation observed for the target gene (Fig.…”