The branch-thorn occurrence of Lycium ruthenicum is associated with leaf DNA hypermethylation in response to soil water content

Yang, An‐Gang; Qi, Xinyu; Wang, Qinmei; Wang, Hao; Wang, Yucheng; Li, Lujia; Wen, Liu; Qiao, Yang

doi:10.1007/s11033-021-07004-6

Cited by 3 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Epigenetic modifications are considered an alternative mechanism by which plants withstand stress without changing nucleotides in the DNA sequence (Kinoshita & Seki, 2014; Kovalchuk et al, 2003; Meyer, 2015; Shi et al, 2018). The MASP technique has been shown to be an effective molecular tool that can be used in plants with little genome data sequence information (Li et al 2011, Yang et al, 2022). Our study used the MSAP to analyse the methylation status of CCGG sequences distributed globally in the plant genome.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of chronic radiation on the flax (Linum usitatissimum L.) genome grown for six consecutive generations in the radioactive Chernobyl area

Jopčík

Libantová

Lancíková

2022

Physiologia Plantarum

View full text Add to dashboard Cite

The growth of plants under chronic radiation stress in the Chernobyl area may cause changes in the genome of plants. To assess the extent of genetic and epigenetic changes in nuclear DNA, seeds of the annual crop flax (Linum usitatissimum L.) of the Kyivskyi variety, sown 21 years after the accident and grown for six generations in radioactive (RAD) and remediated (REM) fields were analysed. Flaxseed used for sowing first generation, which served as a reference (REF), was also analysed. The AFLP (Amplified Fragment Length Polymorphism) revealed a higher number of specific EcoRI–MseI loci (3.4‐fold) in pooled flaxseed samples harvested from the RAD field compared with the REM field, indicating a link between the mutation process in the flax genome and the ongoing adaptation process. MSAP (Methylation‐Sensitive Amplified Polymorphism) detecting EcoRI–MspI and EcoRI–HpaII loci in flax nuclear DNA genome showed no significant differences in methylation level, reaching about 33% in each of the groups studied. On the other hand, significant changes in the DNA methylation pattern of flaxseed samples harvested from the RAD field compared with controls were detected. Pairwise FST comparison revealed within both, EcoRI–MspI and transformed methylation‐Sensitive data sets more than a 3‐fold increase of genetic divergence in the RAD field compared with both controls. These results indicate that the nuclear genome of flax exposed to chronic radiation for six generations has more mutations and uses DNA methylation as one of the adaptation mechanisms for sustainability under adverse conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

“…to be an effective molecular tool that can be used in plants with little genome data sequence information , Yang et al, 2022.…”

Section: Discussionmentioning

confidence: 99%

Effect of chronic radiation on the flax (Linum usitatissimum L.) genome grown for six consecutive generations in the radioactive Chernobyl area

Jopčík

Libantová

Lancíková

2022

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…Raspberry and rose prickles are modified glandular trichomes continuing to grow and eventually hardening into their final prickle morphologies [ 9 ]. The Lycium ruthenicum branch-thorns occur in response to drought and protect the corresponding axillary buds against drought stress [ 10 ]. Transcription factors such as MYB, bHLH, and TCP are involved in the development of prickles [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Gleditsia sinensis Thorns at Different Stages of Development

Xiao

Zhao

Wang

et al. 2023

Plants

View full text Add to dashboard Cite

G. sinensis thorn (called “zào jiǎo cì”, ZJC) has important medicinal and economic value, however, little is known about the molecular mechanisms behind the development of ZJC. In this study, we measured the content of soluble sugar and starch during the growth and development of the thorn, and performed transcriptome sequencing of the thorn segment, non-thorn segment, apex, and root tip at five distinct stages of thorn formation. The results showed that, with the growth of ZJC, the soluble sugar content of the roots, hypocotyls, thorn stems, thornless stems, leaves, and the starch content of the roots and leaves all firstly increased and then decreased after the basic structure of thorns was formed; the soluble sugar content and starch content of ZJC showed an overall downward trend (decreased by 59.26% and 84.56%, respectively). Myb-like, YABBY2, Growth-regulating factor 3, TCP2, Zinc transporter 8, and another 25 genes may be related to the maintenance and growth of thorns. Gene Ontology (GO) enrichment analysis of differentially expressed genes (DEGs) between stems with thorn and thorn-free stems found that a significant number of DEGs were annotated with terms related to the positive regulation of development, heterochronic (GO:0045962), the positive regulation of photomorphogenesis (GO:2000306), and other biological process (BP) terms. The developmental initiation regulation of ZJC may be regulated by TCP transcription factors (TFs). Eight genes were selected randomly to validate the RNA-seq results using real-time quantitative PCR (RT-qPCR) and they indicated that the transcriptome data were reliable. Our work provided a comprehensive review of the thorn development of G. sinensis.

show abstract

Sucrose promotes branch-thorn occurrence of Lycium ruthenicum through dual effects of energy and signal

Qiao

et al. 2023

Tree Physiology

View full text Add to dashboard Cite

Lycium ruthenicum is an important eco-economic thorny shrub. In this study, L. ruthenicum plants of a clone showed two types of ‘less leaves without thorn’ and ‘more leaves with thorns’ under the same condition after transplanting. Microscopic observation revealed that apical buds of the thornless (Thless) and thorny (Thorny) branches should be selected as materials for further study. RNA-Seq analysis showed that KEGG pathway of Starch and sucrose metabolism and DEGs of SUT13, SUS, TPP and TPS were significantly up-regulated in the Thorny. The results of qRT-PCR confirmed the accuracy and credibility of the RNA-Seq. The content of sucrose in the Thorny was significantly higher than that in the Thless, but the content of trehalose-6-phosphate was opposite. Leaf-clipping treatments reduced sucrose content and inhibited the occurrence/development of branch-thorns; exogenous sucrose of 16 g/L significantly promoted the occurrence and growth of branch-thorns and the promotion effects were significantly higher than those treated with non-metabolizable sucrose analogs (isomaltolose, melitose). These findings suggested that sucrose might play a dual role of energy and signal in the occurrence of branch-thorns. Higher sucrose supply in apical buds from more leaves promoted the occurrence of branch-thorns via lower content of trehalose-6-phosphate and higher expression levels of SUS, TPP and TPS, whereas less leaves inhibited the occurrence. Molecular hypothesis model of leaf number/sucrose supply regulating the occurrence of branch-thorns in L. ruthenicum was established in the study, which provides foundation for breeding both thornless L. ruthenicum and thornless types of other species.

show abstract

The branch-thorn occurrence of Lycium ruthenicum is associated with leaf DNA hypermethylation in response to soil water content

Cited by 3 publications

References 33 publications

Effect of chronic radiation on the flax (Linum usitatissimum L.) genome grown for six consecutive generations in the radioactive Chernobyl area

Effect of chronic radiation on the flax (Linum usitatissimum L.) genome grown for six consecutive generations in the radioactive Chernobyl area

Comparative Transcriptome Analysis of Gleditsia sinensis Thorns at Different Stages of Development

Sucrose promotes branch-thorn occurrence of Lycium ruthenicum through dual effects of energy and signal

Contact Info

Product

Resources

About