The brain mapping of the retrieval of conditioned taste aversion memory using manganese-enhanced magnetic resonance imaging in rats

Inui-Yamamoto, Chizuko; Yoshioka, Yoshichika; Inui, Tatsuya; Sasaki, Kenji; Ooi, Yasuhiro; Ueda, Kôichiro; Seiyama, Akitoshi; Ohzawa, Izumi

doi:10.1016/j.neuroscience.2010.02.027

Cited by 26 publications

(20 citation statements)

References 28 publications

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“…Notably, although a close relationship exists between activation of DA D 1 receptors signaling and ERK phosphorylation (Girault et al, 2007), in this study we detected changes of ERK phosphorylation in both the AcbSh and AcbC only during CTA expression. This finding appears in agreement with two lines of evidence obtained upon memory retrieval of conditioned taste aversion: (i) the observation of increased c-FOS-like immunoreactivity in both AcbSh and AcbC (Yasoshima et al, 2006) and (ii) the observation of a greater signal intensity of manganese-enhanced functional magnetic resonance imaging (MEMRI) in AcbSh and AcbC of conditioned rats (Inui-Yamamoto et al, 2010). However, no modifications were observed in the CTA acquisition phase in these Acb sub-regions and, as previously mentioned, also no changes in the pattern of NR1, GluR1 nor DARPP-32 phosphorylation were detected in the AcbSh or AcbC.…”

Section: Discussionsupporting

confidence: 88%

Acquisition and expression of conditioned taste aversion differentially affects extracellular signal regulated kinase and glutamate receptor phosphorylation in rat prefrontal cortex and nucleus accumbens

Fenu

Scheggi

Vinci

et al. 2014

Front. Behav. Neurosci.

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Conditioned taste aversion (CTA) can be applied to study associative learning and its relevant underpinning molecular mechanisms in discrete brain regions. The present study examined, by immunohistochemistry and immunocytochemistry, the effects of acquisition and expression of lithium-induced CTA on activated Extracellular signal Regulated Kinase (p-ERK) in the prefrontal cortex (PFCx) and nucleus accumbens (Acb) of male Sprague-Dawley rats. The study also examined, by immunoblotting, whether acquisition and expression of lithium-induced CTA resulted in modified levels of phosphorylation of glutamate receptor subunits (NR1 and GluR1) and Thr 34 -and Thr 75 -Dopamine-and-cAMP-Regulated PhosphoProtein (DARPP-32). CTA acquisition was associated with an increase of p-ERK-positive neurons and phosphorylated NR1 receptor subunit (p-NR1) in the PFCx, whereas p-GluR1, p-Thr 34 -and p-Thr 75 -DARPP-32 levels were not changed in this brain region. CTA expression increased the number of p-ERK-positive neurons in the shell (AcbSh) and core (AcbC) but left unmodified p-NR1, p-GluR1, p-Thr 34 -and p-Thr 75 -DARPP-32 levels. Furthermore, post-embedding immunogold quantitative analysis in AcbSh revealed that CTA expression significantly increased nuclear p-ERK immunostaining as well as p-ERK-labeled axo-spinous contacts. Overall, these results indicate that ERK and NR1, but not GluR1 and DARPP-32, are differentially phosphorylated as a consequence of acquisition and expression of aversive associative learning. Moreover, these results confirm that CTA represents an useful approach to study the molecular basis of associative learning in rats and suggest the involvement of ERK cascade in learning-associated synaptic plasticity.

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Section: Discussionsupporting

confidence: 88%

Acquisition and expression of conditioned taste aversion differentially affects extracellular signal regulated kinase and glutamate receptor phosphorylation in rat prefrontal cortex and nucleus accumbens

Fenu

Scheggi

Vinci

et al. 2014

Front. Behav. Neurosci.

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“…This criterion was based on reports on fractionated MEMRI in rats Inui-Yamamoto et al 2010), mice (Grünecker et al 2010), and on our own pilot studies. A stock solution of 10 mM MnCl 2 (Sigma) in DDW was prepared.…”

Section: Manganese-enhanced Magnetic Resonance Imaging Testsmentioning

confidence: 99%

“…In order to measure and compare the patterns of brain activation in the mice, we adopted the MEMRI protocol to implement the fractionated mode of systemic Mn 2+ administration (Kuo et al 2005;Grünecker et al 2010;Inui-Yamamoto et al 2010). When administered systemically, Mn 2+ reaches the brain and enters the cells via voltage-gated Ca 2+ channels and then propagates transsynaptically.…”

Section: Brain Activation Under Basal Conditions As Measured In Memrimentioning

confidence: 99%

“…Thus, this mode of Mn 2+ application was expected to result in a final spatial distribution pattern that reflected the differential cumulative activation of brain regions that is associated with CTA. Similar protocols have been applied to studying activation of the rat brain by voluntary running (Eschenko et al 2009), auditory stimulation (Yu et al 2011), and retrieval of novel taste (Inui-Yamamoto et al 2010).…”

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confidence: 99%

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Impaired associative taste learning and abnormal brain activation in kinase-defective eEF2K mice

Gildish¹,

Manor²,

David³

et al. 2012

Learn. Mem.

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Memory consolidation is defined temporally based on pharmacological interventions such as inhibitors of mRNA translation (molecular consolidation) or post-acquisition deactivation of specific brain regions (systems level consolidation). However, the relationship between molecular and systems consolidation are poorly understood. Molecular consolidation mechanisms involved in translation initiation and elongation have previously been studied in the cortex using taste-learning paradigms. For example, the levels of phosphorylation of eukaryotic elongation factor 2 (eEF2) were found to be correlated with taste learning in the gustatory cortex (GC), minutes following learning. In order to isolate the role of the eEF2 phosphorylation state at Thr-56 in both molecular and system consolidation, we analyzed cortical-dependent taste learning in eEF2K (the only known kinase for eEF2) ki mice, which exhibit reduced levels of eEF2 phosphorylation but normal levels of eEF2 and eEF2K. These mice exhibit clear attenuation of cortical-dependent associative, but not of incidental, taste learning. In order to gain a better understanding of the underlying mechanisms, we compared brain activity as measured by MEMRI (manganese-enhanced magnetic resonance imaging) between eEF2K ki mice and WT mice during conditioned taste aversion (CTA) learning and observed clear differences between the two but saw no differences under basal conditions. Our results demonstrate that adequate levels of phosphorylation of eEF2 are essential for cortical-dependent associative learning and suggest that malfunction of memory processing at the systems level underlies this associative memory impairment.

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“…Hence, Mn 2+ has been very useful for visualizing the brain architecture. Recent studies have demonstrated the usefulness of Mn 2+ in providing cytoarchitectonic con-trast to the rodent brain [9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

New Method of Manganese-Enhanced Magnetic Resonance Imaging (MEMRI) for Rat Brain Research

et al. 2012

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Manganese (Mn 2+ )-enhanced MRI (MEMRI) is known to provide insight into functional and anatomical biology. However, this method, which uses Mn 2+ as a MRI-detectable contrast agent, has drawbacks such as the toxicity to cells beyond a certain level of Mn 2+ . In this study, we attempt to determine a new method of ICV administration, the optimal concentration of administered Mn 2+ and the optimal MEMRI acquisition time following administration. Male Sprague-Dawley rats were used in the following experimental sessions: (1) intracerebroventricular (ICV) cannula implantation in the region of the cisterna magna, (2) serial dilution of MnCl 2 (20-80 mM), (3) ICV administration of MnCl 2 through the cannula, and (4) T 1 -weighted MRI measurements. We confirmed that cannula implantation in the region of the cisterna magna was a new ICV injection method for the administration of a contrast agent. The optimal concentration for MEMRI was 20/50 mM/µl of MnCl 2 . The MEMRI data acquired at different time points indicate that most signal enhancement is maintained during 14-48 h after contrast agent injection, and 24 h was the optimal time to acquire images of the rat brain. The present study offers optimized parameters for contrast agent injection that would be a good basis for studies using MEMRI to research the rat brain.

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The brain mapping of the retrieval of conditioned taste aversion memory using manganese-enhanced magnetic resonance imaging in rats

Cited by 26 publications

References 28 publications

Acquisition and expression of conditioned taste aversion differentially affects extracellular signal regulated kinase and glutamate receptor phosphorylation in rat prefrontal cortex and nucleus accumbens

Acquisition and expression of conditioned taste aversion differentially affects extracellular signal regulated kinase and glutamate receptor phosphorylation in rat prefrontal cortex and nucleus accumbens

Impaired associative taste learning and abnormal brain activation in kinase-defective eEF2K mice

New Method of Manganese-Enhanced Magnetic Resonance Imaging (MEMRI) for Rat Brain Research

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