The bphDEF meta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphACB genes in Rhodococcus sp. strain RHA1

Masai, Eiji; Sugiyama, Katsumi; Iwashita, Naoko; Shimizu, Satoru; Hauschild, James E.; Hatta, Takashi; Kimbara, Kazuhide; Yano, Keiji; Fukuda, Masao

doi:10.1016/s0378-1119(96)00748-2

Cited by 104 publications

(75 citation statements)

References 27 publications

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“…Again three linear plasmids, pRHL1 (1100 kb), pRHL2 (450 kb) and pRHL3 (330 kb), were identified in the polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1, and it was shown that gene clusters encoding (chloro)biphenyl conversion are localized on the two largest plasmids (Masai et al, 1997). Rhodococcus corallinus B-276 was even found to harbour four linear plasmids, pNC10 (70 kb), pNC20 (85 kb), pNC30 (185 kb) and pNC40 (235 kb).…”

Section: Discussionmentioning

confidence: 99%

“…Plasmids of rhodococci, which, like their Streptomyces counterparts often possess linear topology, frequently encode catabolic genes, enabling their hosts either to grow with unusual carbon and energy sources such as CO 2 / hydrogen (Kalkus et al, 1990), (chloro)biphenyl (Kosono et al, 1997;Masai et al, 1997), and isopropylbenzene (Dabrock et al, 1994;Stecker et al, 2003) or to just convert such compounds as was shown for trichloroethene (Dabrock et al, 1994;Saeki et al, 1999). In addition, linear plasmids of rhodococci were shown to harbour genes for resistance to thallium (Kalkus et al, 1990) and arsenic (Dabrock et al, 1994;Stecker et al, 2003), as well as genes for phytopathogenicity (Maes et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Extremely high ratios of identical positions (96-100 %) were found between the deduced catabolic proteins for isopropylbenzene degradation (ipbA1A2A3BC) encoded on the 210 kb transmissible linear plasmid of R. erythropolis BD2 (Dabrock et al, 1994;Stecker et al, 2003) and the analogous proteins of a linear-plasmidencoded biphenyl degradation pathway in Rhodococcus sp. strain RHA1 (Masai et al, 1997;Shimizu et al, 2001) and of an isopropylbenzene degradation pathway in Rhodococcus sp. strain I1 (GenBank accession no.…”

Section: Introductionmentioning

confidence: 99%

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A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

et al. 2004

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The Gram-positive actinobacterium Rhodococcus opacus 1CP is able to utilize several (chloro)aromatic compounds as sole carbon sources, and gene clusters for various catabolic enzymes and pathways have previously been identified. Pulsed-field gel electrophoresis indicates the occurrence of a 740 kb megaplasmid, designated p1CP. Linear topology and the presence of covalently bound proteins were shown by the unchanged electrophoretic mobility after S1 nuclease treatment and by the immobility of the native plasmid during non-denaturing agarose gel electrophoresis, respectively. Sequence comparisons of both termini revealed a perfect 13 bp terminal inverted repeat (TIR) as part of an imperfect 583/587 bp TIR, as well as two copies of the highly conserved centre (GCTXCGC) of a palindromic motif. An initial restriction analysis of p1CP was performed. By means of PCR and hybridization techniques, p1CP was screened for several genes encoding enzymes of (chloro)aromatic degradation. A single maleylacetate reductase gene macA, the clc gene cluster for 4-chloro-/3,5-dichlorocatechol degradation, and the clc2 gene cluster for 3-chlorocatechol degradation were found on p1CP whereas the cat and pca gene clusters for the catechol and the protocatechuate pathways, respectively, were not. Prolonged cultivation of the wild-type strain 1CP under non-selective conditions led to the isolation of the clc-and clc2-deficient mutants 1CP.01 and 1CP.02 harbouring the shortened plasmid variants p1CP.01 (500 kb) and p1CP.02 (400 kb).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

et al. 2004

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“…More-detailed features of bph genes were reported in Rhodococcus sp. RHA1 Masai et al, 1995Masai et al, , 1997Yamada et al, 1998). In this strain major, a bph gene cluster was on a 1,100 kb large linear plasmid.…”

Section: Pcb Catabolic Genesmentioning

confidence: 99%

“…strain KKS102 (Kikuchi et al, 1994a, b;Kimbara et al, 1989); RHA1-bph: Rhodococcus sp. strain RHA1 (Masai et al, 1997;Yamada et al, 1998); P6-bph: Rhodococcus groberulus P6 (Asturias et al, 1994;Asturias and Timmis, 1993;Peloquin and Greer, 1993); F1-tod: P. putida F1 (Zylstra and Gibson, 1989); JR1-ipb: Pseudomonas sp. strain JR1 (Pflugmacher et al, 1996); IP01-cum: P. fluorescens IP01 (Habe et al, 1996).…”

Section: Pcb Catabolic Genesmentioning

confidence: 99%

Biochemical and genetic bases of microbial degradation of polychlorinated biphenyls(PCBs).

Furukawa

2000

J. Gen. Appl. Microbiol.

104

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Rhodococcus

Jones

Goodfellow

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Rho.do.coc'cus. Gr. n. rhodon the rose; N.L. masc. n. coccus (from Gr. masc. n. kokkos grain, seed) coccus; N.L. masc. n. Rhodococcus a red coccus. Actinobacteria / Actinobacteria / Corynebacteriales / Nocardiaceae / Rhodococcus Aerobic, Gram‐stain‐positive to Gram‐stain‐variable, nonmotile actinomycetes that are usually partially acid–alcohol‐fast at some stage of the growth cycle. Rods to extensively branched substrate mycelium may be formed . In all strains the growth cycle begins with the coccus or short rod stage, with different organisms showing a succession of more or less complex morphological stages by which the completion of the cycle is achieved. Cocci may germinate into short rods, form filaments with side projections, show elementary branching, or, in the most differentiated forms, produce extensively branched hyphae. The next generation of cocci and short rods are formed by fragmentation of the rods, filaments, and hyphae. Some strains form sparse microscopically visible aerial hyphae, which may be branched, or aerial synnemata consisting of unbranched filaments that coalesce and project upwards. Colonies may be rough, smooth, or mucoid and pigmented buff, cream, orange, red, or yellow, although colorless variants occur. Chemo‐organotrophic with an oxidative type of metabolism. Catalase‐positive, arylsulfatase‐negative, and sensitive to lysozyme. Most strains grow well on standard media between 15 and 40°C, and use a wide range of organic compounds as sole sources of carbon for energy and growth. DNA G + C content ( mol %): 63–73 (HPLC, T m ). Type species : Rhodococcus rhodochrous (Zopf 1889) Tsukamura 1974, 43 VP .

show abstract

The bphDEF meta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphACB genes in Rhodococcus sp. strain RHA1

Cited by 104 publications

References 27 publications

A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

Biochemical and genetic bases of microbial degradation of polychlorinated biphenyls(PCBs).

Rhodococcus

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