The bovine <i>fatty acid binding protein 4</i> gene is significantly associated with marbling and subcutaneous fat depth in Wagyu x Limousin F<sub>2</sub> crosses

Michal, Jennifer J.; Zhang, Zhiwu; Gaskins, C. T.; Jiang, Zhihua

doi:10.1111/j.1365-2052.2006.01464.x

Cited by 141 publications

(140 citation statements)

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“…This hypothesis is supported by the following facts: the porcine FABP4 is closely associated to the FAT1 locus on chromosome 4, and the FAT1 has an important effect on meat marbling and growth (Mercadé et al, 2006). Furthermore, integrated analyses of both genetic map and radiation hybrid (RH) map indicated that the bovine FABP4 gene falls into the QTL interval for marbling on chromosome 14 (Michal et al, 2006). Interestingly, the same functional QTL has been detected on sheep chromosome 9, which is homologous to bovine chromosome 14 (Barillet et al, 2005).…”

Section: Discussionsupporting

confidence: 55%

The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds

Xu¹,

Tang²,

Zhang³

et al. 2011

CZECH J ANIM SCI

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ABSTRACT:The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality traits in sheep. The FABP4 cDNA was cloned by RT-PCR method, and the sequence analysis showed that the open reading frame of sheep FABP4 is 399 bp and codes 132 amino acids. A mutation (A/G) detected in intron 1 of FABP4 gene was studied in 286 lambs of three Chinese native sheep breeds by PCR-SSCP procedure. Significant statistical association results revealed that AA genotype conferred higher tenderness (P < 0.05), muscle marbling score (P < 0.05) and intramuscular fat content (IMF; P < 0.05). Thus we suggested that the genotype AA could be regarded as a molecular marker for LT meat tenderness and IMF content in sheep.

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Section: Discussionsupporting

confidence: 55%

The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds

Xu¹,

Tang²,

Zhang³

et al. 2011

CZECH J ANIM SCI

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“…In fact, CEBPA and FABP4 mRNA levels in the SAT were increased in the fat pigs, without noticeable changes in the LL muscle. FABP4 protein level has been associated with pig's growth stage (Cho et al, 2011), backfat thickness (Michal et al, 2006) and IMF content . Moreover, FABP4 could be perceived as a marker for adipocyte number (Hocquette et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

Lopes

Costa

et al. 2014

Animal

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The production of pork with high amounts of intramuscular fat (IMF) without an increase in subcutaneous fat is highly desirable for the pig industry and consumers. Herein, we question the impact of dietary protein reduction (18% v. 13%) on fat deposition in the subcutaneous adipose tissue (SAT) and longissimus lumborum (LL) muscle using genetically diverse pigs for body fatness (lean v. fat). A clear effect of genotype was observed on plasma insulin (P = 0.004) and leptin (P < 0.001), as well as on backfat thickness (P < 0.001), with the fat pigs having higher values. Accordingly, IMF was higher in the fat pigs, when compared with their lean counterparts (P = 0.003), which was supported by enlarged adipocytes (P < 0.001). The area of lipid droplets within the LL fibres (P = 0.039) and extramyocellular lipids number (P = 0.017) were increased in pigs fed reduced protein diets, regardless of genotype, which is consistent with higher levels of plasma triacylglycerols (P = 0.002). The gene-expression pattern of lipogenic factors in the SAT was distinct from the LL muscle. In the SAT, PPARG expression was similar among genotypes (P > 0.05), whereas in the LL muscle it was higher in the lean pigs (P = 0.023), especially when fed on low protein diet (P = 0.057). The CEBPA and FABP4 mRNA levels were increased in the SAT of fat pigs (P < 0.001), without changes in the LL muscle (P > 0.05). The influence of diet on FABP4 expression in the SAT was dependent on pig's genetic background (P = 0.005). In conclusion, fat deposition was clearly influenced by genotype and, to a lesser extent, by dietary protein level, the SAT being more sensitive than the LL muscle. One can speculate that the pathways involved in lipid metabolism are downregulated in intramuscular adipocytes when compared with SAT fat cells. This result might be a direct consequence of the relatively low proportion of adipocytes found in the LL muscle.

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“…However, this polymorphism was significantly associated with marbling and subcutaneous fat depth in Wagyu × Limousin F2 population (Michal et al, 2006) and in Korean native cattle (Cho et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…A/G transition in the FABP3 gene described by Wu et al (2005); 4. G/C transversion in the FABP4 gene described by Michal et al (2006).…”

mentioning

confidence: 99%

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ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traitsin Jersey cows

Kulig¹,

Kowalewska-Łuczak²,

Kmiec³

et al. 2010

CZECH J ANIM SCI

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Milk components originating from blood plasma substrates are synthesized in epithelial cells of the mammary gland. Milk lipids are synthesized from fatty acids which bind to specific proteins – FABPs (fatty acid binding proteins). FABPs are a family of small cytoplasmic proteins; nine members of the family have been identified so far (FABP1–FABP9) (Chmurzyńska et al., 2006). Their main roles include fatty acid uptake, transport and metabolism. FABPs can modulate the fatty acid concentration in cells and therefore they affect different cellular processes, especially lipid metabolism. FABP3 and FABP4 are present in tissues with a high demand for fatty acids, such as heart muscle, skeletal muscles, lactating mammary gland, liver or adipose tissue (Roy et al., 2003). FABP3 gene was mapped to bovine chromosome 2 (Calvo et al., 2004), where QTLs affecting milk fat yield and content were described (Khatkar et al., 2004). FABP4 gene was mapped to BTA14 (Michal et al., 2006), which is very rich in QTLs for milk production traits (Khatkar et al., 2004). Fatty acid transport is assisted by the specific proteins called FATPs (fatty acid transport proteins). This protein group includes SLC27A3 (solute carrier family 27, member 3). It belongs to the family of proteins that facilitate long-chain fatty acid transport across the cytoplasmic membrane. Another protein with similar functions is ANXA9 (annexin A9), the member of Ca2+ and phospholipid-binding protein family (Calvo et al., 2006b). Genes encoding SLC27A3 and ANXA9 were mapped to chromosome 3, within the region where QTLs for milk fat content and the other milk traits have been mapped. Both of the above-mentioned genes are expressed in the mammary gland (Calvo et al., 2006b). The polymorphic sites (SNPs – single nucleotide polymorphisms) within the bovine FABP3, FABP4, SLC27A3 and ANXA9 genes were identified (Wu et al., 2005; Calvo et al., 2006b; Michal et al., 2006; Cho et al., 2008). Associations between SNPs in these genes and milk production traits in cattle have not been reported so far. However, polymorphism in the FABP4 gene has been significantly associated with carcass traits in cattle (Michal et al., 2006; Cho et al., 2008). Due to their expression sites, physiological properties and chromosomal localisation, the described genes might be considered as candidate genes for milk production traits. The aim of this study was to determine allele and genotype frequencies and to establish possible associations between the ANXA9, SLC27A3, FABP3 and FABP4 SNPs, and selected milk traits in Jersey cows.

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The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyu x Limousin F₂ crosses

Cited by 141 publications

References 12 publications

The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds

The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traitsin Jersey cows

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The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyu x Limousin F2 crosses

Cited by 141 publications

References 12 publications

The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds

The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traitsin Jersey cows

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The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyu x Limousin F₂ crosses