The boron-curcumin complex in the determination of trace amounts of boron

Hayes, M.R.; Metcalfe, J. Stanley

doi:10.1039/an9628700956

Cited by 66 publications

(8 citation statements)

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“…Hot water-soluble boron was extracted by the method of Berger and Truog (1944) and the boron was determined using the curcumin complex (Hayes and Metcalfe 1962). Hot 0.01 M CaC12-extractable boron was determined using the method described by Bingham (1982) except that the suspensions were refluxed for 10 min and the boron concentrations in the filtrates were determined by ICP-AES.…”

Section: Soil Analysesmentioning

confidence: 99%

Evaluation of selected extractants for boron in some Queensland soils

Aitken¹,

Jeffrey²,

Compton³

1987

Soil Res.

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Glasshouse and laboratory studies were undertaken to evaluate a range of extractants for determining the boron status of a suite of predominantly acidic soils. In addition to hot water (commonly used as an extractant for soil boron), hot 0.01 M CaCl,, 0.05 M mannitol/O.Ol M CaCI,, 0.05 M mannitol (buffered at pH 7 . 9 , 1 M NH,OAc, 5% ( v h ) glycerol and 0.01 M tartaric acid were assessed as extractants since these reagents can complex boron.Sunflower (Helianthus annuus cv. Hysun 31) was grown with three levels of applied boron (0, 0.5 and 1.0 kg B ha-') in each of 20 soils. Relative dry matter yield, tissue boron concentration, boron uptake and relative boron uptake of control plants (0 kg B hal) were determined. Both hot water and hot 0.01 M CaClz extractable boron were related to each of these plant parameters, whereas boron extracted by the other procedures was not.Levels of boron extracted with mannitol and glycerol were very low in comparison to those displaced from the soil by the refluxing procedures. The inability of mannitol to extract boron from these soils is discussed in relation to the chemistry of mannitol-boron complexes, and it is suggested that mannitol would not be an effective extractant for boron in acid soils.Soil solution extracts (obtained after equilibration at 10 kPa matric suction) had concentrations < 0.1 ~g B mll . Because of the low concentrations and narrow range across the 20 soils, soil solution boron was not related to plant growth parameters obtained from the glasshouse study.

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Section: Soil Analysesmentioning

confidence: 99%

Evaluation of selected extractants for boron in some Queensland soils

Aitken¹,

Jeffrey²,

Compton³

1987

Soil Res.

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“…One milliliter of the 6 to 1 dilution was used for boron determination by a modification of the colorimetric method of Hays and Metcalfe (1962).…”

Section: Experimental Procedures and Resultsmentioning

confidence: 99%

Interstitial Water Chemistry Deep Sea Drilling Project, Leg 5

Presley¹,

Goldhaber²,

Kaplan³

1970

Initial Reports of the Deep Sea Drilling Project

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“…Also boron deficiency leads to inhibition of growth of shoot meristems as it is a structural constituent of cell walls ( Hu and Brown, 1994 ; Apostol and Zwiazek, 2004 ). As curcumin can form a complex with boron ( Hayes and Metcalfe, 1962 ) and was shown to regulate calcium-related processes and mobilization in human cell cultures ( Kliem et al, 2012 ; Mayadevi et al, 2012 ), it is possible that the curcumin present in ProCesA8-B:DCS_CURS2 poplars makes calcium and boron unavailable, causing deficiencies followed by shoot tip necrosis. Due to their underdeveloped root system, the uptake of calcium and boron from the soil in ProCesA8-B:DCS_CURS2 poplars could additionally be compromised.…”

Section: Discussionmentioning

confidence: 99%

Engineering Curcumin Biosynthesis in Poplar Affects Lignification and Biomass Yield

et al. 2022

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Lignocellulosic biomass is recalcitrant toward deconstruction into simple sugars mainly due to the presence of lignin. By engineering plants to partially replace traditional lignin monomers with alternative ones, lignin degradability and extractability can be enhanced. Previously, the alternative monomer curcumin has been successfully produced and incorporated into lignified cell walls of Arabidopsis by the heterologous expression of DIKETIDE-CoA SYNTHASE (DCS) and CURCUMIN SYNTHASE2 (CURS2). The resulting transgenic plants did not suffer from yield penalties and had an increased saccharification yield after alkaline pretreatment. Here, we translated this strategy into the bio-energy crop poplar. Via the heterologous expression of DCS and CURS2 under the control of the secondary cell wall CELLULOSE SYNTHASE A8-B promoter (ProCesA8-B), curcumin was also produced and incorporated into the lignified cell walls of poplar. ProCesA8-B:DCS_CURS2 transgenic poplars, however, suffered from shoot-tip necrosis and yield penalties. Compared to that of the wild-type (WT), the wood of transgenic poplars had 21% less cellulose, 28% more matrix polysaccharides, 23% more lignin and a significantly altered lignin composition. More specifically, ProCesA8-B:DCS_CURS2 lignin had a reduced syringyl/guaiacyl unit (S/G) ratio, an increased frequency of p-hydroxyphenyl (H) units, a decreased frequency of p-hydroxybenzoates and a higher fraction of phenylcoumaran units. Without, or with alkaline or hot water pretreatment, the saccharification efficiency of the transgenic lines was equal to that of the WT. These differences in (growth) phenotype illustrate that translational research in crops is essential to assess the value of an engineering strategy for applications. Further fine-tuning of this research strategy (e.g., by using more specific promoters or by translating this strategy to other crops such as maize) might lead to transgenic bio-energy crops with cell walls more amenable to deconstruction without settling in yield.

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The boron-curcumin complex in the determination of trace amounts of boron

Cited by 66 publications

References 0 publications

Evaluation of selected extractants for boron in some Queensland soils

Evaluation of selected extractants for boron in some Queensland soils

Interstitial Water Chemistry Deep Sea Drilling Project, Leg 5

Engineering Curcumin Biosynthesis in Poplar Affects Lignification and Biomass Yield

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