The bone mineralization density distribution as a fingerprint of the mineralization process

Ruffoni, Davide; Fratzl, Peter; Roschger, Paul; Klaushofer, Klaus; Weinkamer, Richard

doi:10.1016/j.bone.2007.01.012

Cited by 201 publications

(213 citation statements)

References 29 publications

Supporting

Mentioning

194

Contrasting

Order By: Relevance

“…As bone mineral content is a determinant of bone strength (Currey, 1984;Ruffoni et al, 2007), alterations in tissue mineral distribution within trabeculae and across anatomical locations might also be indicative of reduced mechanical integrity. Indeed previous computational studies have predicted that increased heterogeneity within trabeculae results in signifi cantly increased stiffness (Van der Linden et al, 2001) and adaptive modelling will likely occur leading to trabecular thinning, bone loss and trabecular perforation (Mulvihill et al, 2008;Van der Linden et al, 2004).…”

Section: Heterogeneity Of Bone Mineral During Oestrogen Defi Ciencymentioning

confidence: 99%

“…It is known that bone mineral is heterogeneously distributed across different anatomical regions of the healthy skeleton (Nazarian et al, 2007), due to normal variations in functional remodelling (Tsubota et al, 2009) and tissue mineralisation kinetics (Akkus et al, 2003;Ruffoni et al, 2007). Furthermore remodelling activity is variable across anatomical locations of the proximal femur (Tsubota et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

Gleeson²,

Browne³

et al. 2011

eCM

View full text Add to dashboard Cite

Heterogeneity of bone mineral during oestrogen defi ciency European Cells and Materials Vol. 21 2011 (pages 396-406) ISSN 1473-2262 AbstractAlthough osteoporosis reduces overall bone mass causing bone fragility, recent studies report that the remaining bone tissue is signifi cantly stiffer. Preliminary studies indicate that alterations in bone tissue mineral content might explain these changes, albeit that other studies report conflicting observations. The objective of this study is to quantify whether the distribution of bone tissue mineral is altered during oestrogen defi ciency. Individual trabeculae were harvested from the proximal femur of 7 ovariectomised sheep (OVX), sacrifi ced 12 months postsurgery, and 5 age-matched controls. Mineral content (wt% Ca) was determined using a quantitative backscattered scanning electron microscopy imaging approach. Mineral heterogeneity within individual trabeculae was compared by calculating the full width at half maximum (FWHM) of mineral density distributions. Mean calcium content, the spatial distribution of mineral within trabeculae and the inter-trabecular variation between regions of proximal femora were also compared. Oestrogen defi ciency increased mineral heterogeneity within individual trabeculae compared to healthy controls, as measured by FWHM (3.57 ± 0.68 vs. 3.17 ± 0.36 wt% Ca, p = 0.04). I n particular mineral variability increased between superficial and deep regions of trabeculae of OVX animals (p = 0.04). Interestingly, mineralisation variability between greater and lesser trochanters (i.e. intertrochanteric fracture line) was increased in OVX compared to CON, as indicated by a greater % difference in the standard deviation of trabecular mineral content (77.11 ± 11.70 vs. 45.64 ± 23.70 %, p = 0.03). Such changes are undetectable by evaluating the mean mineral content of bone tissue, but may contribute to changes in bone mechanical strength following osteoporotic bone loss.

show abstract

Section: Heterogeneity Of Bone Mineral During Oestrogen Defi Ciencymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

Gleeson²,

Browne³

et al. 2011

eCM

View full text Add to dashboard Cite

show abstract

“…BMDD produced by qBEI is a frequency distribution which quantifies the heterogeneous Ca content in bone. It is also a fingerprint of of the mineralization process and provides the indirect information about the history of bone remodelling activities and bone turnover rate (Roschger et al, 2003;Ruffoni et al, 2007).…”

Section: Qebi Of Mineralized Bone Tissuementioning

confidence: 99%

“…In contrast, a higher values of Ca width means the distribution gets broader, reflecting an in a increase in the heterogeneity of mineralization. If bone tissue was experienced a high bone turnover rate, the BMDD distribution will shift to lower mineral concentration values due to a less mineralized matrix (Ruffoni et al, 2007;Busse et al, 2009;Ciarelli et al, 2009). Conversely, when the bone turnover rate is reduced, the BMDD distribution will shift to higher mineral concentration values and the width of BMDD histogram become narrow.…”

Section: Qebi Of Mineralized Bone Tissuementioning

confidence: 99%

“…The bone mineralization density distribution (BMDD), produced by qBEI, enables investigation and comparison of mineralization within bone samples at the microscopic scale across different anatomical site and individuals, and captures heterogeneity. It generally provides the mineral distribution within the trabeculae and the differences in degree of mineralization of bone matrix as well as indirectly providing information about bone remodelling history (Ruffoni et al, 2007). The theory of this method is based on the intensity of the backscattered electrons being proportional to the weight percentage of the mineral (calcium) in bone tissue (Roschger et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A study of human and bovine trabecular bones using nanoindentation and quantitative backscattered electron imaging

Lin¹

View full text Add to dashboard Cite

Osteoporosis is a disease with which bone progressively loses density and becomes fragile, leading to high risk of bone fracture. It is a major health issue in Australia, especially in the elderly population. Early awareness about the disease can help find effective medication and reduce bone fractures with appropriate lifestyle changes. In medical diagnosis, bone mineral density (BMD) measurement is used. However, thereare increased evidences to demonstrate that BMD measurement alone may be insufficient to detect the pathological changes in bone that caused by the disease. Therefore, it is essential to gain a more complete understanding of the bone properties and their effect on osteoporosis.Bone is a hierarchical composite material, which mainly consists of mineral apatite and organic matrix. Previous studies found that osteoporosis or aging could cause microstructural changes in trabecular bones of a proximal femur, the most common site for osteoporosis, and an increased degree of anisotropy was observed in osteoporotic bone properties. To identify the risk of fracture occurrence in the proximal femur as well as to prevent bone fracture, it is important to understand the relationship between bone mineral composition and bone mechanical properties. This thesis thus focused on the characterisation of the mechanical properties and mineral composition of trabecular bone, in order to address several key issues relating to the causes of osteoporosis, including: how does trabecular bone respond to different loading directions? how do bone cysts (which are the implication of pathological scenario due to the trabecular fracture) affect the mechanical properties and the mineral composition of trabeculae ? how does the mineral content affect the mechanical properties of trabeculae across different trabecular types and pathology scenarios ?To answer those questions, trabecular bone samples harvested from bovine and human femoral heads were prepared for testing. Nanoindentation was first used to characterise the mechanical behaviour of trabeculae and atomic force microscopy (AFM) was employed to examine the bone surface tomography. Quantitative backscattered electron imaging (qBEI) was utilized for determining the bone mineral density distribution and the mineral content.ii The results showed that at microscopic scale longitudinal trabeculae were stiffer and harder than transverse trabeculae, because those bones were more highly mineralized.Longitudinal trabeculae also exhibited greater resistance to plastic deformation. The difference in mechanical properties between longitudinal and transverse trabeculae is partially attributed to the difference in their mineral compositions. To further understand the mechanical behaviour of trabeculae, longitudinal and transverse trabeculae were tested in two orthogonal directions. Results showed that both longitudinal and transverse trabeculae had greater elastic modulus in axial direction than in radial direction. Because the axial loading direction is axially aligned with th...

show abstract

Mechanical Design of Biomineralized Tissues. Bone and Other Hierarchical Materials

Fratzl

2008

Biomineralization

View full text Add to dashboard Cite

show abstract

The bone mineralization density distribution as a fingerprint of the mineralization process

Cited by 201 publications

References 29 publications

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

A study of human and bovine trabecular bones using nanoindentation and quantitative backscattered electron imaging

Mechanical Design of Biomineralized Tissues. Bone and Other Hierarchical Materials

Contact Info

Product

Resources

About