The bone-healing effect of a xenograft in a rat calvarial defect model

Develıoğlu, Hakan; Saraydın, Serpil Ünver; Kartal, Ünal

doi:10.4012/dmj.28.396

Cited by 43 publications

(35 citation statements)

References 23 publications

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“…It was reported that Unilab Surgibone was tested in critical sized defects in rats for one month and the biomaterial was seen biocompatible and useful. 32 Moreover, in the case of the cytotoxic activities, the xenograft evaluated here showed no cytotoxicity for the cells. Therefore, it seems as a useful material.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Bone Repair Capacity and the Cytotoxic Properties of a Particular Xenograft: An Experimental Study in Rats

Saraydın¹,

Develıoğlu²

2011

Turkiye Klinikleri J Med Sci

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one repair is a major challenge for reconstructive surgery. The reconstruction of segmental mandibular defects following trauma or ablative surgery remains controversial because no single method results in the replacement of tissue that precisely matches the quantitiy or structural qualities of autogenous mandibular bone. Moreover, current A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Bo ne grafts ha ve be en wi dely used in os se o us de fects in me di ci ne and dentistry (e.g. pe ri o don to logy, imp lan to logy). This study aims to as sess bo ne and tis su e res pon se to a xe nog raft (Uni lab Sur gi bo ne) af ter the first and third month of imp lan ta ti on and its cyto to xi city in vit ro. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : Fif te en rats, each of which ha ving a 5 mm cri ti cal-si ze, symmet ri cal, cir cu lar de fects in the cor pus of the man dib le we re rec ru i ted. The ex pe ri men tal de fects we re fil led with the bi o ma te ri al whe re as de fects on the op po si te si de we re used as con trols (sham-ope ra ted gro up). Af ter his to lo gi cal fol low-up, se ri al sec ti ons we re pre pa red for eva lu a ti on un der light micros co pe. The cyto to xi city of xe nog raft was exa mi ned in vit ro. R Re e s su ul lt t: : Xe nog rafts did not show any cyto to xi city for the cells. The re was no cal lus for ma ti on in the imp lan ta ti on si te at the first and third months, and Uni lab Sur gi bo ne ma te ri al was sur ro un ded with fib ro us tis su e. Den se col la geno us tis su e was ob ser ved in the con trol si te at the first and third months. C Co on nc c l lu u s si i o on n: : Alt ho ugh there was no evi den ce of bo ne bu il ding, the bi o ma te ri al se ems bi o com pa tib le and can be con si de red as a spa ce fil ler for such app li ca ti ons in den tistry.K Ke ey y W Wo or rd ds s: : Bo ne and bo nes; cyto to xi city tests, immu no lo gic; den tal prost he sis re pa ir; os te o ge ne sis Ö ÖZ ZE ET T A Am ma aç ç: : Ke mik greft ler ke mik de fekt le rin de tıp ve diş he kim li ğin de yay gın ola rak kul la nıl -mak ta dır (ör ne ğin pe ri o don to lo ji, imp lan to lo ji). Bu ça lış ma bir xe nog raf ta (Uni lab Sur gi bo ne) imp lan tas yon dan son ra ki birinci ve üçüncü ay da ke mik ve do ku ya nı tı nı de ğer len dir me yi amaç -la mak ta dır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Man di bu la kor pu sun da 5 mm kri tik bo yut lu si met rik da i re sel defekt le re sa hip 15 sı çan ça lış ma ya alın mış tır. Ça lış ma ya esas de fekt ler bi yo ma ter yal ile dol du ru lurken, kar şı ta raf ta ki de fekt ler kon trol ola rak kul la nıl dı. His to lo jik ta kip son ra sın da, ışık mik ros ko bu al tın da de ğer len dir me için se ri ke sit ler ha zır lan dı. Xe nog raft si to tok si si te si in vit ro in ce len di. B Bu ul l--g gu u l la ar r: : Xe nog raft lar hüc re ler için her han gi bir si to tok si si te gös ter me di. İmp lan tas yon ala nın da birinci ve üçüncü ay da kal lus oluş ma dı, ve Uni lab Sur gi ...

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Bone Repair Capacity and the Cytotoxic Properties of a Particular Xenograft: An Experimental Study in Rats

Saraydın¹,

Develıoğlu²

2011

Turkiye Klinikleri J Med Sci

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“…As there is a well-documented model for the assessment of bone healing, experimental studies on the rat skull is the gold standard for testing tissue reaction to root canal filling material [3,13,14]. For each group, the control orifice was 1 of the 4 orifices made in the calvaria, which was free of any tested material.…”

Section: Discussionmentioning

confidence: 99%

The influence of three endodontic sealers on bone healing: an experimental study

et al. 2016

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“…In order to evaluate the ability of BMP-7 derived-peptide chitosan nanometer hydroxyapatite collagen composite to promote bone regeneration, we select the rat skull defect as experimental animal model, which is characterized by the advantages of simple, standard and reproducible. [11] Based on the results of the preliminary experiment, the researchers selected 1 mg as the amount of the composite polypeptide, and polypeptide chitosan nanometer hydroxyapatite biomimetic collagen composites and pure chitosan nanometer hydroxyapatite biomimetic collagen composites alone were respectively implanted into the rat skull defects. General observation, three dimensional reconstruction of CT and histological observation were performed at 6 and 12 weeks after the operation.…”

Section: Discussionmentioning

confidence: 99%

Experimental study on advanced bone regeneration by BMP-7 derived-peptide chitosan nanometer hydroxyapatite collagen biomimetic composite

Feng

Yang-de

2016

DCC

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Objective: To investigate the effect of BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composite on repairing rat critical-sized cranial defects. Methods: The chitosan nanometer hydroxyapatite collagen composite was prepared and the microcosmic appearance of the composite was observed by scanning electron microscope. The BMP-7 derived-peptide was introduced into the composite by vacuum adsorption. The released peptide content from the scaffold was detected using high performance liquid chromatography at different set times. Critical-sized cranial defects were created on both sides of the parietal bone in 24 adult Sprague-Dawley rats. The BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composites were implanted on the right side as experimental group and the left side was implanted with chitosan nanometer hydroxyapatite biomimetic collagen composites alone as control group. The rats of both groups were killed in batch respectively after 6 and 12 weeks. Macroscopic observation, three-dimensional reconstruction of computed tomography (CT) and histological observation were performed on these samples. Results: The results of scanning electron microscope showed that the surface of the scaffold was porous. The releasing character of BMP-7 derived-peptide belonged to slow release. The result of animal experiment showed that the BMP-7 derived-peptide chitosan nanometer hydroxyapatite biomimetic collagen composite could more effectively promote the repair of cranial bone defects comparing with the chitosan nanometer hydroxyapatite biomimetic collagen composite alone. The difference was statistically significant (p < .05). Conclusions: The BMP-7 derived-peptide chitosan nanometer hydroxyapatite collagen biomimetic composite can effectively promote bone regeneration of bone defects. The composite is a kind of ideal scaffold material for bone tissue engineering.

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The bone-healing effect of a xenograft in a rat calvarial defect model

Cited by 43 publications

References 23 publications

Evaluation of the Bone Repair Capacity and the Cytotoxic Properties of a Particular Xenograft: An Experimental Study in Rats

Evaluation of the Bone Repair Capacity and the Cytotoxic Properties of a Particular Xenograft: An Experimental Study in Rats

The influence of three endodontic sealers on bone healing: an experimental study

Experimental study on advanced bone regeneration by BMP-7 derived-peptide chitosan nanometer hydroxyapatite collagen biomimetic composite

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