The Bladder Microbiome, Metabolome, Cytokines, and Phenotypes in Patients with Systemic Lupus Erythematosus

Liu, Fengping; Du, Jingjie; Zhai, Qixiao; Hu, Jialin; Miller, Aaron W.; Ren, Tao; Feng, Yangkun; Peng, Jiang; Hu, Lei; Sheng, Jiayi; Gu, Chaoqun; Ren, Yan; Lv, Longxian; Wolfe, Alan J.; Feng, Na

doi:10.1128/spectrum.00212-22

Cited by 12 publications

(21 citation statements)

References 59 publications

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“…A recent study evaluated active SLE patients (score SLEDAI-2K = 16.16 ± 24.31 points) and reported a negative correlation between Megamonas abundance in the bladder microbiome and C3 complement levels. Notably, HCQ dosage did not affect microbial communities [31]. Conversely, gut Megamonas has been reported to be depleted in the gut of SLE patients and positively correlated with helper T cells 17 (Th17) [32].…”

Section: Discussionmentioning

confidence: 94%

Megamonas funiformis, Plasma Zonulin, and Sodium Intake Affect C3 Complement Levels in Inactive Systemic Lupus Erythematosus

et al. 2023

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The etiology of systemic lupus erythematosus (SLE) remains unclear, with both genetic and environmental factors potentially contributing. This study aimed to explore the relationship among gut microbiota (GM), intestinal permeability, and food intake with inflammatory markers in inactive SLE patients. A total of 22 women with inactive SLE and 20 healthy volunteers were enrolled, and dietary intake was assessed through 24-h dietary recalls. Plasma zonulin was used to evaluate intestinal permeability, while GM was determined by 16S rRNA sequencing. Regression models were used to analyze laboratory markers of lupus disease (C3 and C4 complement and C-reactive protein). Our results showed that the genus Megamonas was significantly enriched in the iSLE group (p < 0.001), with Megamonas funiformis associated with all evaluated laboratory tests (p < 0.05). Plasma zonulin was associated with C3 levels (p = 0.016), and sodium intake was negatively associated with C3 and C4 levels (p < 0.05). A combined model incorporating variables from each group (GM, intestinal permeability, and food intake) demonstrated a significant association with C3 complement levels (p < 0.01). These findings suggest that increased Megamonas funiformis abundance, elevated plasma zonulin, and higher sodium intake may contribute to reduced C3 complement levels in women with inactive SLE.

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Section: Discussionmentioning

confidence: 94%

Megamonas funiformis, Plasma Zonulin, and Sodium Intake Affect C3 Complement Levels in Inactive Systemic Lupus Erythematosus

et al. 2023

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“…These microbiomes are essential for immune homeostasis. Mounting evidence supports the notion that dysbiosis in the blood (5), gut (6)(7)(8)(9)(10)(11)(12)(13), oral cavity (10,(14)(15)(16)(17), skin (18), and bladder (19) is associated with SLE. It is worth noting that most previous studies have reported gut dysbiosis in SLE patients, but several have demonstrated that a balanced gut microbiome is accompanied by an imbalanced microbiome in other body niches, such as the blood (5) and oral cavity (16), when the same cohort of SLE patients and healthy controls (HC) were involved in the studies.…”

Section: Introductionmentioning

confidence: 88%

Alterations of the fecal and vaginal microbiomes in patients with systemic lupus erythematosus and their associations with immunological profiles

et al. 2023

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BackgroundExploring the human microbiome in multiple body niches is beneficial for clinicians to determine which microbial dysbiosis should be targeted first. We aimed to study whether both the fecal and vaginal microbiomes are disrupted in SLE patients and whether they are correlated, as well as their associations with immunological features.MethodsA group of 30 SLE patients and 30 BMI-age-matched healthy controls were recruited. Fecal and vaginal samples were collected, the 16S rRNA gene was sequenced to profile microbiomes, and immunological features were examined.ResultsDistinct fecal and vaginal bacterial communities and decreased microbial diversity in feces compared with the vagina were found in SLE patients and controls. Altered bacterial communities were found in the feces and vaginas of patients. Compared with the controls, the SLE group had slightly lower gut bacterial diversity, which was accompanied by significantly higher bacterial diversity in their vaginas. The most predominant bacteria differed between feces and the vagina in all groups. Eleven genera differed in patients’ feces; for example, Gardnerella and Lactobacillus increased, whereas Faecalibacterium decreased. Almost all the 13 genera differed in SLE patients’ vaginas, showing higher abundances except for Lactobacillus. Three genera in feces and 11 genera in the vagina were biomarkers for SLE patients. The distinct immunological features were only associated with patients’ vaginal microbiomes; for example, Escherichia−Shigella was negatively associated with serum C4.ConclusionsAlthough SLE patients had fecal and vaginal dysbiosis, dysbiosis in the vagina was more obvious than that in feces. Additionally, only the vaginal microbiome interacted with patients’ immunological features.

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“…Specifically, the bacterial DNA isolation, 16 S rRNA sequencing, and bioinformatics ( Liu et al, 2023 ). The extraction of bacterial DNA from urine, high-throughput sequencing, and bioinformatics analysis have been comprehensively described in our previous research ( Liu et al, 2022 ). Here, we provide a brief overview: Following PCR amplification of bacterial DNA, extraction was carried out using AMpure XP magnetic beads (Beckman Coulter, Indianapolis, IN, USA).…”

Section: Methodsmentioning

confidence: 99%

“…When conducting a microbiome study, it is essential to account for potential sources of contamination and validate the accuracy of the sequencing method. In our study, we included negative control and positive samples to assess environmental DNA contamination ( Liu et al, 2022 ). Amplification was performed using primers 319F and 806R, targeting the highly sensitive V3–V4 region of the 16S rRNA gene.…”

Section: Methodsmentioning

confidence: 99%

Impact of coexisting type 2 diabetes mellitus on the urinary microbiota of kidney stone patients

Li,

Tang,

et al. 2024

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Objectives Type 2 diabetes mellitus (T2DM) commonly complicates kidney stone disease (KSD). Our objective is to investigate the variations in the urinary microbiota between individuals with KSD alone and those with KSD plus T2DM. This exploration could have implications for disease diagnosis and treatment strategies. Methods During lithotripsy, a ureterscope was employed, and 1 mL of urine was collected from the renal pelvis after bladder disinfection. Sequencing targeting the V3–V4 hypervariable region was performed using the 16S rRNA and Illumina Novaseq platform. Results The Shannon index showed a significant decrease in the KSD plus T2DM group compared to the KSD-only group (false discovery rate = 0.041). Principal Coordinate Analysis (PCoA) demonstrated a distinct bacterial community in the KSD plus T2DM group compared to the KSD-only group (false discovery rate = 0.027). The abundance of Sphingomonas, Corynebacterium, and Lactobacillus was significantly higher in the KSD plus T2DM group than in the KSD-only group (false discovery rate < 0.05). Furthermore, Enhydrobacter, Chryseobacterium, and Allobaculum were positively correlated with fasting blood glucose and HbA1c values (P < 0.05). Conclusions The urinary microbiota in the renal pelvis exhibits differences between patients with KSD plus T2DM and those with KSD alone. Further studies employing animal models are necessary to validate these distinctions, potentially paving the way for therapeutic developments based on the urinary microbiota.

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The Bladder Microbiome, Metabolome, Cytokines, and Phenotypes in Patients with Systemic Lupus Erythematosus

Abstract: Contrary to dogma, the human urinary bladder possesses its own unique bacterial community with alteration of composition associated with disease states. Systemic lupus erythematosus (SLE) is a complex autoimmune disease often characterized by kidney impairment.

Cited by 12 publications

References 59 publications

Megamonas funiformis, Plasma Zonulin, and Sodium Intake Affect C3 Complement Levels in Inactive Systemic Lupus Erythematosus

Megamonas funiformis, Plasma Zonulin, and Sodium Intake Affect C3 Complement Levels in Inactive Systemic Lupus Erythematosus

Alterations of the fecal and vaginal microbiomes in patients with systemic lupus erythematosus and their associations with immunological profiles

Impact of coexisting type 2 diabetes mellitus on the urinary microbiota of kidney stone patients

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