The biotin interference within interference suppressed immunoassays

Abstract

Background Reports of false laboratory findings due to a biotin supplementation have raised concerns about the safety of immunoassays. According to current research, biotin is known to cause interference in immunoassays. Since up to 70% of medical decisions are based on laboratory results and the significantly increased intake of biotin supplements in the recent years, the reliability of immunoassays is essential. Methods To evaluate this reliability two experiments were conducted. In the first experiment 59 interference suppressed immunoassays of the manufacturer Roche Diagnostics were examined regarding their sensitivity to a biotin interference. In the second experiment the pharmacokinetic of biotin was examined by supplementing volunteers with biotin. Results A combination of the results of both experiments suggests that a biotin interference in laboratory findings is probable. Contrary to the current state of research on sandwich immunoassays, falsely elevated test results occur more frequently than falsely low results. Conclusion The interference suppressed immunoassays have shown in the experiment that they are susceptible to a biotin interference. Therefore, laboratory institutions, medical staff and patients must be aware of the possibility of a biotin interference. As a result, Roche Diagnostics may consider reviewing the interference suppression and their indications of the tests.

Characterization of the role of Facebook groups for patients who use scalp cooling therapy: a survey study

“…No clinical trials were found. Doses and duration varied by case report Some degree of improvement in hair growth or nail growth after 10 days to 6 months of treatment - Evidence only in populations with pathology for either poor hair or nail growth (e.g., uncombable hair syndrome, valproic acid-induced alopecia) - Can interfere with laboratory immunoassays (e.g., concentration of immunosuppressive drugs, thyroid function tests, cardiac enzymes, hepatitis, vitamin d) [ 30 – 33 ] - AAD** recommended discontinuation of biotin for promotion of hair regrowth ( https://dignicap.com/patient-instructions/ ) [ 34 ] * DHT dihydrotestosterone ** AAD American Academy of Dermatology …”

“…- Can interfere with laboratory immunoassays (e.g., concentration of immunosuppressive drugs, thyroid function tests, cardiac enzymes, hepatitis, vitamin d) [ 30 – 33 ]…”

Effects of High-Biotin Sample Interference on Antibody Concentrations in Sandwich Immunoassays

“…Therefore, changes in the measured values of sandwich immunoassays can lead to false low or high values in the measured results, depending on the concentrations and the relationships among the proteins, biotin, and reagents in the assays using biotin-avidin/streptavidin. Kabiri et al [22] studied biotin interference in protein measurements and concluded that biotin in the sample leads to false high values in sandwich immunoassays. Liu et al [23] observed that biotin in the sample led to a false negative result because the assay failed to detect the target Ab even though it was present in the sample.…”

“…Approximately 85% of chemiluminescence immunoassays are based on biotin-avidin/streptavidin and are used by more than twothirds of laboratories in China [20,21]. All laboratories using signal amplification through biotin-streptavidin interactions face the threat of misdiagnosis due to biotin interference caused by excessive biotin consumption [22,23].…”

Coordinate-Dependent Drift-Diffusion Reveals the Kinetic Intermediate Traps of Top7-Based Proteins

“…Nevertheless, it could represent a growing concern of an emerging interference in clinical immunoassays . Biotinylation might reduce the sensibility of an ELISA assay by preventing protein binding, interfering in the laboratory immunoassay results. , In most cases, researchers focus on the overall aspect of enzymatic biotin-labeling, and the remaining biomolecular behavior is not always mentioned. It is possible that just a few studies investigated, at the level of individual residues, the underlying basis of the changes induced by biotinylation with respect to the biotin-labeled experiments.…”