The Biomechanics of eyelid tarsus tissue

Sun, Michelle T.; Pham, Diana T.; O’Connor, Andrea J.; Wood, John; Casson, Robert J.; Selva, Dinesh; Costi, John J.

doi:10.1016/j.jbiomech.2015.05.037

Cited by 18 publications

(15 citation statements)

References 43 publications

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“…We found that an average internal pore size of 25 ± 17 lm was able to successfully support the growth of human orbital skin fibroblasts in vitro which would likely translate to ongoing proliferation in vivo. There have been only two previous studies investigating the use of bioengineered scaffolds as a tarsal substitute, although both pre-dated our study on the normal biomechanical properties of tarsus tissue [8], which was the first of its kind. Zhou et al [6] studied polyhydroxyalokonoate scaffolds implanted in rats, and compared with commercial acellular dermal matrices (ADM) and blank defect controls.…”

Section: Discussionmentioning

confidence: 99%

“…Tensile tests were carried out using an Instron Microtester 5848 (Instron, Melbourne, Victoria, Australia) with a temperature controlled water bath (BioPuls, Instron, Melbourne, Victoria, Australia), under the same operating conditions as previously described for tarsus tissue [8]. Scaffolds made using rectangular moulds were mounted between two pneumatic clamps and lowered into the water bath containing PBS at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…The raw data from the 15th cycle was used to calculate the mechanical properties via a custom-written MATLAB function (MATLAB R2014b, The MathWorks Inc., Natick, MA, USA). The initial and final moduli were calculated from the initial linear region and the final linear region of the stress-strain curve, respectively, using a moving linear fit with the highest coefficient of determination [8]. The elongation was calculated from the point of intersection of the two moduli tangents.…”

Section: Methodsmentioning

confidence: 99%

“…Having established the biomechanical properties of fresh human tarsal tissue [8], in this study we report the development of a novel bioengineered chitosan hydrogel scaffold designed biomechanically mimic human tarsus tissue, onto which we were able to successfully seed human eyelid fibroblast cells. Naturally-derived polymers such as chitosan have excellent potential as tissue engineered scaffolds for soft tissues due to their high water content and biomimetic properties [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Development of Macroporous Chitosan Scaffolds for Eyelid Tarsus Tissue Engineering

Sun

O’Connor

Milne

et al. 2019

Tissue Eng Regen Med

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BACKGROUND: Reconstruction of large eyelid defects remains challenging due to the lack of suitable eyelid tarsus tissue substitutes. We aimed to evaluate a novel bioengineered chitosan scaffold for use as an eyelid tarsus substitute. METHODS: Three-dimensional macroporous chitosan hydrogel scaffold were produced via cryogelation with specific biomechanical properties designed to directly match characteristics of native eyelid tarsus tissue. Scaffolds were characterized by confocal microscopy and tensile mechanical testing. To optimise biocompatibility, human eyelid skin fibroblasts were cultured from biopsy-sized samples of fresh eyelid skin. Immunological and gene expression analysis including specific fibroblast-specific markers were used to determine the rate of fibroblast de-differentiation in vitro and characterize cells cultured. Eyelid skin fibroblasts were then cultured over the chitosan scaffolds and the resultant adhesion and growth of cells were characterized using immunocytochemical staining. RESULTS: The chitosan scaffolds were shown to support the attachment and proliferation of NIH 3T3 mouse fibroblasts and human orbital skin fibroblasts in vitro. Our novel bioengineered chitosan scaffold has demonstrated biomechanical compatibility and has the ability to support human eyelid skin fibroblast growth and proliferation. CONCLUSIONS: This bioengineered tissue has the potential to be used as a tarsus substitute during eyelid reconstruction, offering the opportunity to pre-seed the patient's own cells and represents a truly personalised approach to tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of Macroporous Chitosan Scaffolds for Eyelid Tarsus Tissue Engineering

Sun

O’Connor

Milne

et al. 2019

Tissue Eng Regen Med

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“… 3 Additionally, tarsal plate biomechanics plays an important role in the normal function of the eyelid and the anterior segment of the eye. 32 A recent report by Kymionis et al 33 described the failure of a corneal cross-linking treatment in a keratoconus patient with FES. Several studies 28 , 34 have found some relationship between FES and keratoconus, which is also a degenerative disease of the cornea with distinct biomechanical alterations.…”

Section: Discussionmentioning

confidence: 99%

Does severity of dermatochalasis in aging affect corneal biomechanical properties?

Atalay¹,

Gürez²,

Kirgiz³

et al. 2016

CIA

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PurposeThe aim of this study was to investigate the possibility of a relationship between corneal biomechanical properties and different grades of dermatochalasis.Patients and methodsPatients were assigned to four groups according to the severity of their dermatochalasis: normal (Group 1), mild (Group 2), moderate (Group 3), and severe (Group 4). An Ocular Response Analyzer device was used to measure corneal hysteresis (CH), corneal resistance factor (CRF), and corneal-compensated intraocular pressure (IOPcc).ResultsWe found no significant differences in the mean values of the CH, CRF, and IOPcc of all groups (P=0.75, P=0.93, and P=0.11, respectively). However, CH and IOPcc were negatively correlated in Group 1, Group 2, and Group 3 patients (P=0.013, r=−0.49; P=0.015, r=−0.52; and P=0.011, r=−0.47, respectively), but this correlation was not apparent in the Group 4 patients (P=0.57, r=0.12). CRF and IOPcc were correlated, but only in Group 4 (P=0.001, r=0.66).ConclusionSevere dermatochalasis was associated with altered corneal biomechanical properties. Some of the important visual consequences of dermatochalasis and related diseases (such as floppy eyelid syndrome) can be understood by considering corneal biomechanical alterations.

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Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

Yan

et al. 2023

Bioengineering & Transla Med

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Reconstruction of posterior lamellar eyelids remains challenging due to their delicate structure, highly specialized function, and cosmetic concerns. Current clinically available techniques for posterior lamellar reconstruction mainly focus on reconstructing the contour of the eyelids. However, the posterior lamella not only provides structural support for the eyelid but also offers a smooth mucosal surface to facilitate globe movement and secrete lipids to maintain ocular surface homeostasis. Bioengineered posterior lamellar substitutes developed via acellular or cellular approaches have shown promise as alternatives to current therapies and encouraging outcomes in animal studies and clinical conditions. Here, we provide a brief reference on the current application of autografts, biomaterials, and tissue‐engineered substitutes for posterior lamellar eyelid reconstruction. We also shed light on future challenges and directions for eyelid regeneration strategies and offer perspectives on transitioning replacement strategies to regeneration strategies for eyelid reconstruction in the future.

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The Biomechanics of eyelid tarsus tissue

Cited by 18 publications

References 43 publications

Development of Macroporous Chitosan Scaffolds for Eyelid Tarsus Tissue Engineering

Development of Macroporous Chitosan Scaffolds for Eyelid Tarsus Tissue Engineering

Does severity of dermatochalasis in aging affect corneal biomechanical properties?

Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

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