The biomechanical, chemical, and physiological adaptations of the eggs of two Australian megapodes to their nesting strategies and their implications for extinct titanosaur dinosaurs

Abstract

Megapodes are galliform birds endemic to Australasia and unusual amongst modern birds in that they bury their eggs for incubation in diverse substrates and using various strategies. Alectura lathami and Leipoa ocellata are Australian megapodes that build and nest in mounds of soil and organic matter. Such unusual nesting behaviors have resulted in particular evolutionary adaptations of their eggs and eggshells. We used a combination of scanning electron microscopy, including electron backscatter diffraction and energydispersive X-ray spectroscopy, to determine the fine structure of the eggshells and micro-CT scanning to map the structure of pores. We discovered that the surface of the eggshell of A. lathami displays nodes similar to those of extinct titanosaur dinosaurs from Transylvania and Auca Mahuevo egg layer #4 (AM L#4). We propose that this pronounced nodular ornamentation is an adaptation to an environment rich in organic acids from their nest mound, protecting the egg surface from chemical etching and leaving the eggshell thickness intact. By contrast, L. ocellata nests in mounds of sand with less organic matter in semiarid environments and has eggshells with weakly defined nodes, like those of extinct titanosaurs from AM L#3 that also lived in a semiarid environment. We suggest the internode spaces in both megapode and titanosaur species act as funnels, which concentrate the condensed water vapor between the nodes. This water funneling in megapodes through the layer of calcium phosphate reduces the likelihood of bacterial infection by creating a barrier to microbial invasion. In addition, the accessory layer of both species possesses sulfur, which reinforces the calcium phosphate barrier to bacterial and fungal contamination. Like titanosaurs, pores through the eggshell are Y-shaped in both species, but A. lathami displays unique mid-shell connections tangential to the eggshell surface and that connect some adjacent pores, like the eggshells of titanosaur of AM L#4and Transylvania. The function of these inter-connections is not known, but likely helps the diffusion of gases in eggs buried in environments where occlusion of pores is possible. AbstractMegapodes are galliform birds endemic to Australasia and unusual amongst modern birds in that they bury their eggs for incubation in diverse substrates and using various strategies.Alectura lathami and Leipoa ocellata are Australian megapodes that build and nest in mounds of soil and organic matter. Such unusual nesting behaviors have resulted in particular evolutionary adaptations of their eggs and eggshells. We used a combination of scanning electron microscopy, including electron backscatter diffraction and energy-dispersive X-ray spectroscopy, to determine the fine structure of the eggshells and micro-CT scanning to map the structure of pores. We discovered that the surface of the eggshell of A. lathami displays nodes similar to those of extinct titanosaur dinosaurs from Transylvania and Auca Mahuevo egg layer #4 (AM L#4). We propose that this pronounc...

Eggshell composition and surface properties of avian brood-parasitic species compared with non-parasitic species

“…Whether surface structures of brood-parasitic eggs have evolved to minimize microbial infection has not been investigated. However, eggs under particularly high risk of infection, such as those of compost-nesting malleefowls (Leipoa ocellata), have evolved extremely hydrophobic (water-repellent) eggshell surfaces comprising nanosphere-type structures [36,37]. A less wettable surface causes water to 'bead up' rather than spread out over the shell surface, and in doing so, the water traps harmful microbes in these droplets and minimizes the formation of biofilms [5].…”