Abstract. A hyperspectral imager was used to differentiate herbicide-resistant versus herbicidesusceptible biotypes of the agronomic weed kochia, in different crops in the field at the Southern Agricultural Research Center in Huntley, Montana. Controlled greenhouse experiments showed that enough information was captured by the imager to classify plants as either a crop, herbicidesusceptible or herbicide-resistant kochia. The current analysis is developing an algorithm that will work in more uncontrolled outdoor situations. In overcast conditions, the algorithm correctly identified dicamba-resistant kochia, glyphosate-resistant kochia, and glyphosate-and dicamba-susceptible kochia with 67%, 76%, and 80% success rates, respectively. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.