The biology and methodology of assisted reproduction in deer mice (Peromyscus maniculatus)

Veres, Monika; Duselis, Amanda R.; Graft, Audrey; Pryor, William A.; Crossland, Janet P.; Vrana, Paul B.; Szalai, Gábor

doi:10.1016/j.theriogenology.2011.07.044

Cited by 19 publications

(30 citation statements)

References 26 publications

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“…Due to the dismal survival of oocytes without ZP after vitrification, we focused our IVF and embryonic development studies on the fresh and vitrified oocytes with an intact zona pellucida. Of note, the study on the embryonic development of deer mice is still in its infancy and few in vitro fertilized oocytes could be developed to beyond the 4-cell stage [4,5,29]. Although they are beyond the scope of this work, further studies are warranted to further confirm our observation using oocytes from species with well-established protocols of IVF allowing for embryonic development to obtain blastocysts for further implementation to produce viable and normal animals.…”

Section: Resultsmentioning

confidence: 82%

The crucial role of zona pellucida in cryopreservation of oocytes by vitrification

et al. 2015

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Mammalian oocytes have a proteinaceous hydrogel-like outer shell known as the zona pellucida (ZP) that semi-encloses their plasma membrane and cytoplasm. In this study, we cryopreserved mouse oocytes either with or without ZP by vitrification. Our results show that the presence of an intact ZP could significantly improve the post-vitrification survival of oocytes to 92.1% from 13.3% for oocytes without ZP. Moreover, there was no significant difference in embryonic development between fresh and cryopreserved oocytes with ZP after in vitro fertilization (IVF). Further atomic force microscopy (AFM) analysis showed that the intact oocytes with ZP have an elastic modulus that is more than 85 times higher than that of oocytes without ZP. This may partially explain the important role of ZP in protecting the oocytes by resisting the mechanical stress due to possible ice formation during cryopreservation by vitrification. Collectively, this study reveals a new biophysical role of ZP during vitrification of oocytes and suggests microencapsulation of the many mammalian cells without a ZP in ZP-like hydrogel is an effective strategy to improve their survival post cryopreservation by vitrification.

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Section: Resultsmentioning

confidence: 82%

The crucial role of zona pellucida in cryopreservation of oocytes by vitrification

et al. 2015

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“…63 In brief, 12-24-week-old virgin Peromyscus maniculatus bairdii females were hormone primed with 15 IU of PMSG followed by a second 15 IU of PMSG 24 h later. At 48 h post the second PMSG, the females were further hormone primed with 15 IU of hCG.…”

Section: Isolation Of Peromyscus Oocytesmentioning

confidence: 99%

Biotransport Phenomena in Freezing Mammalian Oocytes

Yang

Veres²,

Szalai³

et al. 2010

Ann Biomed Eng

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Water transport across the cell plasma membrane and intracellular ice formation (IIF)-the two biophysical events that may cause cell injury during cryopreservation-were studied by cryomicroscopy and modeling using mammalian (Peromyscus) oocytes. Unusually high activation energy for water transport across the cell plasma membrane was identified indicating that the water transport process is unusually sensitive to temperature (and cooling rate). Although literally all studies on IIF were conducted using protocols with ice-seeding (seeding extracellular ice usually at ≥-7 °C), it is not used for cell cryopreservation by vitrification that is becoming increasingly popular today. In this article, we show that ice-seeding has a significant impact on IIF. With ice-seeding and cooling at 60 °C/min, IIF was observed to occur over a wide range from approximately -8 to -48 °C with a clear change of the ice nucleation mechanism (from surface- to volume-catalyzed nucleation) at approximately -43 °C. On the contrary, without ice-seeding, IIF occurred over a much narrower range from approximately -19 to -27 °C without a noticeable change of the nucleation mechanism. Moreover, the kinetics of IIF without ice-seeding was found to be strongly temperature (and cooling rate) dependent. These findings indicate the importance of quantifying the IIF kinetics in the absence of ice-seeding during cooling for development of optimal vitrification protocols of cell cryopreservation.

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“…The genuses Peromyscus (also called deer mice because their fur color resembles deer) are indigenous rodents in North American [4], [5]. Peromyscus have been used as the animal model in studies of phylogeography, seciation, chromosomes, and population genetics and evolution because they are not difficult to maintain in an animal facility and most importantly, they are probably more appropriate than inbred laboratory mice for research aimed for medical applications due to their outbred nature as with humans.…”

Section: Introductionmentioning

confidence: 99%

“…PMSG (pregnant mare’s serum gonadotropin) and hCG (human chorionic gonadotropin) are the two hormones that have been used to retrieve oocytes from laboratory mice usually with high yield (more than 20 per animal) [10], [11], [12]. However, due to the large variation in response to hormone stimulation from species to species [12], [13] , the yield of oocyte retrieval from deer mice by superovulation using the two hormones has been dismal according to the literature to date: Less than ∼5 oocytes per animal could be obtained [5], [8]. Consequently, an optimized protocol for isolation of deer mice oocytes, its in vitro fertilization (IVF), and in vitro embryo culture and development has not been well established [4], [9].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Maturation of Cumulus-Oocyte Complexes for Efficient Isolation of Oocytes from Outbred Deer Mice

Choi

2013

PLoS ONE

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BackgroundThe outbred (as with humans) deer mice have been a useful animal model of research on human behavior and biology including that of the reproductive system. One of the major challenges in using this species is that the yield of oocyte isolation via superovulation is dismal according to the literature to date less than ∼5 oocytes per animal can be obtained so far.ObjectiveThe goal of this study is to improve the yield of oocyte isolation from outbred deer mice close to that of most laboratory mice by in vitro maturation (IVM) of cumulus-oocyte complexes (COCs).MethodsOocytes were isolated by both superovulation and IVM. For the latter, COCs were obtained by follicular puncture of antral follicles in both the surface and inner cortical layers of ovaries. Immature oocytes in the COCs were then cultured in vitro under optimized conditions to obtain metaphase II (MII) oocytes. Quality of the oocytes from IVM and superovulation was tested by in vitro fertilization (IVF) and embryo development.ResultsLess than ∼5 oocytes per animal could be isolated by superovulation only. However, we successfully obtained 20.3±2.9 oocytes per animal by IVM (16.0±2.5) and superovulation (4.3±1.3) in this study. Moreover, IVF and embryo development studies suggest that IVM oocytes have even better quality than that from superovulation The latter never developed to beyond 2-cell stage as usual while 9% of the former developed to 4-cells.SignificanceWe have successfully established the protocol for isolating oocytes from deer mice with high yield by IVM. Moreover, this is the first ever success to develop in vitro fertilized deer mice oocytes beyond the 2-cell stage in vitro. Therefore, this study is of significance to the use of deer mice for reproductive biology research.

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The biology and methodology of assisted reproduction in deer mice (Peromyscus maniculatus)

Cited by 19 publications

References 26 publications

The crucial role of zona pellucida in cryopreservation of oocytes by vitrification

The crucial role of zona pellucida in cryopreservation of oocytes by vitrification

Biotransport Phenomena in Freezing Mammalian Oocytes

In Vitro Maturation of Cumulus-Oocyte Complexes for Efficient Isolation of Oocytes from Outbred Deer Mice

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