The biology and function of extracellular vesicles in nasopharyngeal carcinoma (Review)

You, Bo; Shan, Ying; Bao, Lili; Chen, Jing; Liu, Yang; Zhang, Qicheng; Zhang, Wei; Zhang, Zhenxin; Zhang, Jie; Shi, Si; You, Yiwen

doi:10.3892/ijo.2017.4202

Cited by 10 publications

(11 citation statements)

References 118 publications

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“…The same results were achieved in in vitro studies with exosomes derived from metastatic tumor cells [100]. Moreover, EVs from nasopharyngeal carcinoma (NPC) cells are enriched with HCLS1-associated protein X-1 (HAX-1) and could accelerate the proliferation and migration of HUVECs [134].…”

Section: Molecular Cargo Of Tex Influencing Angiogenesis / Tex Conversupporting

confidence: 64%

“…In a variety of cancer types, EVs have been shown to increase tube formation, migration, cell-cell adhesion and proliferation in ECs [100,102,113,[127][128][129][130][131][132][133]. Studies have been performed to determine the relationship between EVs and pathological angiogenesis [134]. It was demonstrated that HUVECs induce pathological angiogenesis [135] via an abundance of genetic information transferred by EVs [136].…”

Section: Molecular Cargo Of Tex Influencing Angiogenesis / Tex Convermentioning

confidence: 99%

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The role of tumor-derived exosomes in tumor angiogenesis and tumor progression

Głuszko

Mirza

Piszczatowska

et al. 2019

Current Issues in Pharmacy and Medical Sciences

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Exosomes, belonging to the group of extracellular bodies, are released by healthy as well as cancerous cells and serve as a communication pathway. Tumor-derived exosomes (TEX) possess the capacity to reprogram the function of normal cells owing to their genetic and molecular cargo. Such exosomes target endothelial cells (among others) in the tumor microenvironment to promote angiogenesis. Blood supply is essential in solid tumor growth and metastasis. The potential of pro-angiogenic changes is enhanced by an increased amount of circulating tumor-derived exosomes in the body fluids of cancer patients. A vascular network is important, since the proliferation, as well as the metastatic spread of cancer cells depends on an adequate supply of oxygen and nutrients, and the removal of waste products. New blood vessels and lymphatic vessels are formed through processes called angiogenesis and lymphangiogenesis, respectively. Angiogenesis is regulated by both activator and inhibitor molecules. Thousands of patients have received anti-angiogenic therapy to date. Despite their theoretical efficacy, anti-angiogenic treatments have not proved beneficial in terms of long-term survival. Tumor-derived exosomes carrying pro-angiogenic factors might be a target for new anti-cancer therapy.

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Section: Molecular Cargo Of Tex Influencing Angiogenesis / Tex Conversupporting

confidence: 64%

Section: Molecular Cargo Of Tex Influencing Angiogenesis / Tex Convermentioning

confidence: 99%

The role of tumor-derived exosomes in tumor angiogenesis and tumor progression

Głuszko

Mirza

Piszczatowska

et al. 2019

Current Issues in Pharmacy and Medical Sciences

View full text Add to dashboard Cite

show abstract

“…The crucial role of EVs in a wide variety of malignancies highlights their potential functions as biomarkers and therapeutic targets. 19 , 20 Hence, the current study set out to investigate the functional interaction of NPC-cell-derived EV-shuttled miR-144 with FBXW7 in relation to NPC tumorigenesis and angiogenesis. Collectively, the experimental data provided evidence suggesting that miR-144 carried by EVs derived from NPC cells contributed to angiogenesis of HUVECs via the upregulation of HIF-1α and VEGF-A through target inhibition of FBXW7, which has the potential to be applied in the development of therapeutic strategies.…”

Section: Discussionmentioning

confidence: 99%

miR-144 delivered by nasopharyngeal carcinoma-derived EVs stimulates angiogenesis through the FBXW7/HIF-1α/VEGF-A axis

Tian

Liu

Wang

et al. 2021

Molecular Therapy - Nucleic Acids

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The current study aimed to explore the role of tumor-derived extracellular vesicles (EVs) in angiogenesis during nasopharyngeal carcinoma (NPC). NPC biopsy specimens were initially collected. Human umbilical vein endothelial cells (HUVECs) were co-cultured with EVs isolated from NPC cells, after which their migration, invasion, as well as vessel-like tube formation were evaluated by Transwell chamber systems and Matrigel-based angiogenesis assays. The pro-angiogenic activities of EVs as well as the candidate microRNA (miRNA or miR) were examined using an in vivo Matrigel angiogenesis model. The results indicated that the levels of miR-144 in the NPC tissues were upregulated when compared to the nasopharyngeal normal tissues in addition to the identification of a positive correlation with the expression of CD31. Moreover, our data indicated that miR-144 was highly enriched in EVs from NPC cells and then ultimately enhanced the migration and invasion of HUVECs and vessel-like tubes in vitro and in vivo . Notably, miR-144 was identified as a mediator in NPC-EV-induced regulatory effects through the inhibition of the target gene FBXW7 and promotion of the transcriptional factor HIF-1α-dependent vascular endothelial growth factor (VEGF-A). Taken together, the key findings of the current study highlighted the role of miR-144 as an extracellular pro-angiogenic mediator in NPC tumorigenesis.

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“…Prior evidence has reported that RUNX2 can promote the occurrence of vascular injury repair (4), while RUNX2 can activate the expression of miR-23a (5). Recently, more studies shed light on the potential of miR-23a acting as biomarkers and therapeutic targets (20,21). Emerging evidence has highlighted that miR-23a can inhibit apoptosis and inflammation during vascular injury, thereby inhibiting vascular injury (10).…”

Section: Discussionmentioning

confidence: 99%

RUNX2 promotes vascular injury repair by activating miR-23a and inhibiting TGFBR2

Cai

Liu

et al. 2021

Ann Transl Med

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Background: Previous evidence has suggested that the transcription factor, runt-related transcription factor 2 (RUNX2), promotes the repair of vascular injury and activates the expression of microRNA-23a (miR-23a). TGF-β receptor type II (TGFBR2) has been found to mediate smooth muscle cells (SMCs) following arterial injury. However, the interactions among RUNX2, miR-23a and TGFBR2 in vascular injury have not been investigated thoroughly yet. Therefore, we aim to explore the mechanism of how RUNX2 triggers the expression of miR-23a and its effects on the repair of vascular injury.Methods: C57BL/6 mice were used to produce a model of arterial injury in vivo. Mouse arterial SMCs were isolated for in vitro cell injury induction by 100 nmol/L tumor necrosis factor-α (TNF-α). Gain-and lossof-function studies were conducted to assess cell viability and apoptosis by using cell counting kit (CCK)-8 assay and flow cytometry respectively. The levels of TNF-α, interleukin-6 (IL-6), and monocyte chemotactic protein-1 (MCP-1) were examined by enzyme-linked immunosorbent assay (ELISA). The interaction between RUNX2 and miR-23a was identified by chromatin immunoprecipitation (ChIP) and dual luciferase reporter assays, while the targeting relationship between miR-23a and TGFBR2 was analyzed by RNA immunoprecipitation (RIP) and dual luciferase reporter assays.Results: Both RUNX2 and miR-23a exhibited low levels of expressions, while TGFBR2 had a high level of expression in mice with induced arterial injury. RUNX2 was found to bind to the promoter of miR-23a and activate miR-23a, while miR-23a targeted TGFBR2. Ectopic RUNX2 expression inhibited inflammatory cell infiltration, and promoted collagen content by upregulating miR-23a and downregulating TGFBR2. Furthermore, the overexpression of RUNX2 increased viability and decreased apoptosis in vascular smooth muscle cells (VSMCs) by activating miR-23a. Conclusions:The overexpression of RUNX2 elevated the expression of miR-23, thus inhibiting TGFBR2 and promoting vascular injury repair.

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The biology and function of extracellular vesicles in nasopharyngeal carcinoma (Review)

Cited by 10 publications

References 118 publications

The role of tumor-derived exosomes in tumor angiogenesis and tumor progression

The role of tumor-derived exosomes in tumor angiogenesis and tumor progression

miR-144 delivered by nasopharyngeal carcinoma-derived EVs stimulates angiogenesis through the FBXW7/HIF-1α/VEGF-A axis

RUNX2 promotes vascular injury repair by activating miR-23a and inhibiting TGFBR2

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