The Biological Role of Glycerol in Yeast Cells. Yeast as Glycerol Producers

Vustin, M. M.

doi:10.1134/s0003683821090088

Cited by 5 publications

(1 citation statement)

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“…Polyhydric alcohols such as glycerol, erythritol, mannitol, or arabitol are the most frequently formed osmoprotectants in the cells of some microorganisms, including the yeast Y. lipolytica [ 11 , 38 ]. However, when exposed to high osmotic pressure, Y. lipolytica preferred to produce erythritol rather than mannitol in order to balance the osmotic pressure between inside and outside of the plasma membrane more effectively [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Sugar Alcohol Sweetener Production by Yarrowia lipolytica Grown in Media Containing Glycerol

Juszczyk,

Rywińska,

Kosicka

et al. 2023

Molecules

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Most of the world’s annual production of mannitol is by chemical means, but, due to increasing demand for natural sweeteners, alternative production methods are being sought. The aim of the study was to screen Yarrowia lipolytica yeast strains and select culture conditions for the efficient and selective biosynthesis of mannitol from glycerol. From 21 strains examined in the shake-flask culture for mannitol biosynthesis from glycerol (100 g/L), three strains were selected—S2, S3, and S4—and further evaluated in batch bioreactor cultures with technical and raw glycerol (150 g/L). The best production parameters were observed for strain S3, which additionally was found to be the most resistant to NaCl concentration. Next, strain S3 was examined in batch culture with regard to the initial glycerol concentration (from 50 to 250 g/L). It was found that the substrate concentrations of 50 and 75 g/L resulted in the highest mannitol selectivity, about 70%. The fed-batch culture system proposed in this paper (performed in two variants in which glycerol was dosed in four portions of about 50 or 75 g/L) resulted in increased mannitol production, up to 78.5 g/L.

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Section: Resultsmentioning

confidence: 99%