The Biological Evaluation of Jellyfish Collagen as a New Research Tool for the Growth and Culture of iPSC Derived Microglia

Mearns‐Spragg, Andrew; Tilman, Jessica; Tams, Daniel; Barnes, Ashley H.

doi:10.3389/fmars.2020.00689

Cited by 17 publications

(11 citation statements)

References 27 publications

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“…In addition, collagen is a promising biopolymer for the removal of skin defects, deforming scars, pigmentation, as well as for the healing of wounds such as extensive burns, tendon surgery, and muscle injuries [ 25 ]. Remarkably, marine collagen exhibited identical properties to human collagen, which has great potential for developing a next-generation matrix for cell culture and regenerative medicine applications [ 26 ], Thus, it is a suitable candidate for hemostatic materials and applications in wound healing [ 27 ], while collagen-based gels play a role in the remodeling phase of the wound healing process by promoting extracellular matrix remodeling and accelerating wound closure of injured tissue [ 28 , 29 ]. In the cosmetic field, collagen can be used as an ingredient in the formulation of beauty products (creams, gels, masks, shampoos), with claimed action against wrinkling and aging, nourish, moisturize, retain moisture, and block ultraviolet (UV) rays [ 12 , 30 , 31 ], Moreover, it can increase the elasticity of the skin, make it soft and shiny, improve fibroblast production and consequently promote the renewal of the extracellular matrix of the skin.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Skin Hydrating Efficacy of Fish Collagen from Greenland Halibut as a High-Value Active Ingredient for Cosmetic Applications

2023

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The industrial processing of fish for food purposes also generates a considerable number of by-products such as viscera, bones, scales, and skin. From a value-added perspective, fish by-products can act also as raw materials, especially because of their collagen content (particularly in fish skin). Interestingly, the potential of marine collagen for cosmetic applications is enormous and, remarkably, the extraction of this protein from fish skins has been established for different species. Using this approach, we investigated the integration of marine collagen (COLRp_I) extracted from the skin of the Greenland halibut as an active ingredient in a cosmetic hydrogel formulation. In this study, extracts of marine collagen at concentrations up to 10 mg/mL showed a non-cytotoxic effect when cultured with fibroblast cells for 3 days. In addition, marine collagen extract, when incorporated into a cosmetic hydrogel formulation, met criterion A of ISO 11930:2019 regarding the efficacy of the preservative system (challenge test). In addition, the cosmetic formulations based on marine collagen at dosages of 0.1, 0.25 and 0.5% were tested in a clinical study on the skin of the forearms of 23 healthy volunteers, showing a sightly hydration effect, suggesting its potential for beauty applications. Moreover, this work illustrates that the circular economy concept applied to the fish processing industry can represent important benefits, at innovation, environmental and economic levels.

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Section: Introductionmentioning

confidence: 99%

In Vivo Skin Hydrating Efficacy of Fish Collagen from Greenland Halibut as a High-Value Active Ingredient for Cosmetic Applications

2023

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“…Several studies claim that jellyfish-derived collagens from various species are analogous to specific collagen types found in mammals, and whilst this may not be entirely true due to differences in amino acid composition, their use as scaffolds in vivo has shown some success in the regeneration of cartilage, bone and vascular tissue [ 28 , 29 , 30 ]. Jellyfish collagen has also been used in 2D and 3D in vitro models of mesenchymal stem cells (MSCs), induced pluripotent stem cells (iPSCs), chondrocytes, chondroprogenitors, osteoblasts, fibroblasts and ovarian cancer cell lines [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Biophysical Properties and Cell Adhesion Interactions of Marine Invertebrate Collagen from Rhizostoma pulmo

Smith

Domingos²,

Richardson³

et al. 2023

Marine Drugs

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Collagen is the most ubiquitous biomacromolecule found in the animal kingdom and is commonly used as a biomaterial in regenerative medicine therapies and biomedical research. The collagens used in these applications are typically derived from mammalian sources which poses sociological issues due to widespread religious constraints, rising ethical concern over animal rights and the continuous risk of zoonotic disease transmission. These issues have led to increasing research into alternative collagen sources, of which marine collagens, in particular from jellyfish, have emerged as a promising resource. This study provides a characterization of the biophysical properties and cell adhesion interactions of collagen derived from the jellyfish Rhizostoma pulmo (JCol). Circular dichroism spectroscopy and atomic force microscopy were used to observe the triple-helical conformation and fibrillar morphology of JCol. Heparin-affinity chromatography was also used to demonstrate the ability of JCol to bind to immobilized heparin. Cell adhesion assays using integrin blocking antibodies and HT-1080 human fibrosarcoma cells revealed that adhesion to JCol is primarily performed via β1 integrins, with the exception of α2β1 integrin. It was also shown that heparan sulfate binding plays a much greater role in fibroblast and mesenchymal stromal cell adhesion to JCol than for type I mammalian collagen (rat tail collagen). Overall, this study highlights the similarities and differences between collagens from mammalian and jellyfish origins, which should be considered when utilizing alternative collagen sources for biomedical research.

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“…The Jellagen ® collagen from R. pulmo (JC) involved in this study has been reported to have potential in culturing human iPSC-derived Microglia (iMGL) and ovarian cancer (OvCa) cell lines [24,28]. In this context, Jellagen ® collagen is of interest as a culture medium for a cytocompatibility analysis, which is an extensively used tool in the evaluation of biomaterials.…”

Section: Introductionmentioning

confidence: 99%

Suitability of R. pulmo Jellyfish-Collagen-Coated Well Plates for Cytocompatibility Analyses of Biomaterials

Ren

Liu

Xiong

et al. 2023

IJMS

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Cytocompatibility analyses of new implant materials or biomaterials are not only prescribed by the Medical Device Regulation (MDR), as defined in the DIN ISO Norm 10993-5 and -12, but are also increasingly replacing animal testing. In this context, jellyfish collagen has already been established as an alternative to mammalian collagen in different cell culture conditions, but a lack of knowledge exists about its applicability for cytocompatibility analyses of biomaterials. Thus, the present study was conducted to compare well plates coated with collagen type 0 derived from Rhizostoma pulmo with plates coated with bovine and porcine collagen. The coated well plates were analysed in vitro for their cytocompatibility, according to EN ISO 10993-5/−12, using both L929 fibroblasts and MC3T3 pre-osteoblasts. Thereby, the coated well plates were compared, using established materials as positive controls and a cytotoxic material, RM-A, as a negative control. L929 cells exhibited a significantly higher viability (#### p < 0.0001), proliferation (## p < 0.01), and a lower cytotoxicity (## p < 0.01 and # p < 0.05)) in the Jellagen® group compared to the bovine and porcine collagen groups. MC3T3 cells showed similar viability and acceptable proliferation and cytotoxicity in all collagen groups. The results of the present study revealed that the coating of well plates with collagen Type 0 derived from R. pulmo leads to comparable results to the case of well plates coated with mammalian collagens. Therefore, it is fully suitable for the in vitro analyses of the cytocompatibility of biomaterials or medical devices.

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The Biological Evaluation of Jellyfish Collagen as a New Research Tool for the Growth and Culture of iPSC Derived Microglia

Cited by 17 publications

References 27 publications

In Vivo Skin Hydrating Efficacy of Fish Collagen from Greenland Halibut as a High-Value Active Ingredient for Cosmetic Applications

In Vivo Skin Hydrating Efficacy of Fish Collagen from Greenland Halibut as a High-Value Active Ingredient for Cosmetic Applications

Characterization of the Biophysical Properties and Cell Adhesion Interactions of Marine Invertebrate Collagen from Rhizostoma pulmo

Suitability of R. pulmo Jellyfish-Collagen-Coated Well Plates for Cytocompatibility Analyses of Biomaterials

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