Abstract:The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low t… Show more
“…Expression studies have shown that YLR162W transcript level was increased by 4.3 folds in cells over-expressing MLH1 [4] i.e., under conditions of elevated spontaneous mutation rate and genomic instability. YLR162W expression was also significantly elevated during high-pressure stress [5], upon Mg 2? starvation [6], during response to a-factor (50 folds) [7] and in stationary phase [8].…”
YLR162W is an uncharacterized Saccharomyces cerevisiae ORF whose transcript level is elevated in cells under environmental stress, during α-factor response and in stationary phase. We obtained a partial cDNA clone of YLR162W by subtractive hybridization cloning of genes that were not expressed in a CoCl(2) resistant DNA synthesis mutant but expressed in its wild type counterpart. Our studies demonstrated that YLR162W transcript level was reduced in BY4741 cells upon exposure to the hypoxia mimetic agent CoCl(2), and continuous expression of full length YLR162W from a plasmid borne copy of the gene rendered BY4741 cells extremely susceptible to the hypoxic conditions induced by CoCl(2). At initial time points following the induction of YLR162W expression, cell cycle progression was inhibited with the emergence of a distinct sub-G1 peak indicative of apoptotic cells, mitochondrial membrane potential was also decreased along with an increase in the fraction of cells permeable to propidium iodide; none of the above was further affected by CoCl(2). The up-regulation of Ylr162wp in cells exposed to environmental stress and in non-replicating cells appears to be related to its growth inhibitory properties presented in this report.
“…Expression studies have shown that YLR162W transcript level was increased by 4.3 folds in cells over-expressing MLH1 [4] i.e., under conditions of elevated spontaneous mutation rate and genomic instability. YLR162W expression was also significantly elevated during high-pressure stress [5], upon Mg 2? starvation [6], during response to a-factor (50 folds) [7] and in stationary phase [8].…”
YLR162W is an uncharacterized Saccharomyces cerevisiae ORF whose transcript level is elevated in cells under environmental stress, during α-factor response and in stationary phase. We obtained a partial cDNA clone of YLR162W by subtractive hybridization cloning of genes that were not expressed in a CoCl(2) resistant DNA synthesis mutant but expressed in its wild type counterpart. Our studies demonstrated that YLR162W transcript level was reduced in BY4741 cells upon exposure to the hypoxia mimetic agent CoCl(2), and continuous expression of full length YLR162W from a plasmid borne copy of the gene rendered BY4741 cells extremely susceptible to the hypoxic conditions induced by CoCl(2). At initial time points following the induction of YLR162W expression, cell cycle progression was inhibited with the emergence of a distinct sub-G1 peak indicative of apoptotic cells, mitochondrial membrane potential was also decreased along with an increase in the fraction of cells permeable to propidium iodide; none of the above was further affected by CoCl(2). The up-regulation of Ylr162wp in cells exposed to environmental stress and in non-replicating cells appears to be related to its growth inhibitory properties presented in this report.
In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (3 weeks journals ‐ search completed 7th. Dec. 2004)
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