The biological activity of cationic liposomes in drug delivery and toxicity test in animal models

Qi, Panpan; Cao, Mei; Song, Lingyun; Chen, Chong; Liu, Mingdong; Li, Ningzhe; Wu, Daoyan; Peng, Jingshan; Hu, Guoku; Zhao, Jian

doi:10.1016/j.etap.2016.09.015

Cited by 18 publications

(9 citation statements)

References 21 publications

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“…However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26][27][28]. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative.…”

Section: Discussioncontrasting

confidence: 66%

Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation

Ahn

Kim

et al. 2020

Biomater Res

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Background Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. Methods The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Results The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. The enhanced permeability through micropores was confirmed by Franz diffusion cell. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. In addition, the permeation rate was higher in pig skin than in human skin. Conclusions We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery.

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Section: Discussioncontrasting

confidence: 66%

Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation

Ahn

Kim

et al. 2020

Biomater Res

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“…Although cationic liposomes afford high transfection activity, their use in intravenously administered formulations require major attention since these nanoparticles can interact electrostatically with various negatively charged surfaces such as red blood cells and plasma proteins. The interaction can promote erythrocyte lysis and release of hemoglobin [65]. For this, in vitro evaluation of formulation-induced erythrocyte hemolysis is considered a simple and reliable measure for estimating blood compatibility of biomaterials.…”

Section: Discussionmentioning

confidence: 99%

Dual functionalized liposome-mediated gene delivery across triple co-culture blood brain barrier model and specific in vivo neuronal transfection

Rodrigues

Oue

Banerjee

et al. 2018

Journal of Controlled Release

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Gene therapy has become a promising approach for neurodegenerative disease treatment, however there is an urgent need to develop an efficient gene carrier to transport gene across the blood brain barrier (BBB). In this study, we strategically designed dual functionalized liposomes for efficient neuronal transfection by combining transferrin (Tf) receptor targeting and enhanced cell penetration utilizing penetratin (Pen). A triple cell co-culture model of BBB confirmed the ability of the liposomes to cross the barrier layer and transfect primary neuronal cells. In vivo quantification of PenTf-liposomes demonstrated expressive accumulation in the brain (12%), without any detectable cellular damage or morphological change. The efficacy of these nanoparticles containing plasmid β-galactosidase in modulating transfection was assessed by β-galactosidase expression in vivo. As a consequence of accumulation in the brain, PenTf-liposomes significantly induced gene expression in mice. Immunofluorescence studies of brain sections of mice after tail vein injection of liposomes encapsulating pDNA encoding GFP (pGFP) illustrate the superior ability of dual-functionalized liposomes to accumulate in the brain and transfect neurons. Taken together, the multifunctional liposomes provide an excellent gene delivery platform for neurodegenerative diseases.

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“…[130] Thus, POPC and DOTAP lipids were chosen as Ru(III) complexes stabilizing agents on the basis of their proved biocompatibility. [77,105,106,131,132] In particular, POPC liposomes are efficiently used in a broad range of biotechnological applications, [133] while liposomes formed by the cationic lipid DOTAP showed noteworthy transfection efficiency both in vitro and in vivo, [131] probably due to the favourable interactions with the negatively charged membranes.…”

Section: Liposome-based Systems Containing Nucleolipid or Aminoacyl Lmentioning

confidence: 99%

Anticancer Ruthenium(III) Complexes and Ru(III)-Containing Nanoformulations: An Update on the Mechanism of Action and Biological Activity

et al. 2019

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The great advances in the studies on metal complexes for the treatment of different cancer forms, starting from the pioneering works on platinum derivatives, have fostered an increasingly growing interest in their properties and biomedical applications. Among the various metal-containing drugs investigated thus far, ruthenium(III) complexes have emerged for their selective cytotoxic activity in vitro and promising anticancer properties in vivo, also leading to a few candidates in advanced clinical trials. Aiming at addressing the solubility, stability and cellular uptake issues of low molecular weight Ru(III)-based compounds, some research groups have proposed the development of suitable drug delivery systems (e.g., taking advantage of nanoparticles, liposomes, etc.) able to enhance their activity compared to the naked drugs. This review highlights the unique role of Ru(III) complexes in the current panorama of anticancer agents, with particular emphasis on Ru-containing nanoformulations based on the incorporation of the Ru(III) complexes into suitable nanocarriers in order to enhance their bioavailability and pharmacokinetic properties. Preclinical evaluation of these nanoaggregates is discussed with a special focus on the investigation of their mechanism of action at a molecular level, highlighting their pharmacological potential in tumour disease models and value for biomedical applications.

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The biological activity of cationic liposomes in drug delivery and toxicity test in animal models

Cited by 18 publications

References 21 publications

Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation

Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation

Dual functionalized liposome-mediated gene delivery across triple co-culture blood brain barrier model and specific in vivo neuronal transfection

Anticancer Ruthenium(III) Complexes and Ru(III)-Containing Nanoformulations: An Update on the Mechanism of Action and Biological Activity

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