2020
DOI: 10.1016/j.molcel.2019.10.006
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The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription

Abstract: Highlights d SRP RNA exhibits robust co-transcriptional folding invariant to transcription rates d SRP RNA adopts a non-native obligatory intermediate prior to its functional fold d The obligatory intermediate enables SRP RNA maturation during early transcription d Altering the non-native-to-native transition in SRP RNA impacts cell viability

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Cited by 23 publications
(26 citation statements)
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“…2). ffs (SRP_RNA) plays a critical role in co-translational protein targeting and delivery to cellular membranes by binding with signal recognition particle (SRP) (Flores and Ataide, 2018;Fukuda et al, 2020). rnpB (RNase_P_RNA) (Kirsebom, 2002;2007) associates with endoribonuclease P (RNase P) to process tRNA precursors and generate mature 5' termini.…”
Section: Rnas In the Ngago Complexmentioning
confidence: 99%
“…2). ffs (SRP_RNA) plays a critical role in co-translational protein targeting and delivery to cellular membranes by binding with signal recognition particle (SRP) (Flores and Ataide, 2018;Fukuda et al, 2020). rnpB (RNase_P_RNA) (Kirsebom, 2002;2007) associates with endoribonuclease P (RNase P) to process tRNA precursors and generate mature 5' termini.…”
Section: Rnas In the Ngago Complexmentioning
confidence: 99%
“…In the past several years, bacterial transcription systems have been used to develop high-resolution biochemical and biophysical methods for studying RNA folding and function (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). In many cases, biochemical tools that were developed for studying the process of bacterial transcription have been successfully repurposed for these applications.…”
Section: Introductionmentioning
confidence: 99%
“…Similar intermediates were detected during folding of E. coli signal recognition particle RNA and transfer-messenger RNA, which likewise adopt long-range helices in their native structures. Thus, the strategic position of a pause site located between the upstream and downstream portions of long-range helices can foster the formation of meta-stable, non-native structures, that protect the 5ʹ-portions of the helices from being trapped within stable, nonproductive structures [ 21 , 22 ].…”
Section: Introductionmentioning
confidence: 99%
“…Single-molecule techniques, such as optical-trapping assays and single-molecule fluorescence resonance energy transfer (smFRET) approaches monitor co-transcriptional RNA folding events in real time and provide information about the formation of native and nonproductive meta-stable RNA intermediate structures and their dependence on transcription rates and pause sites, which previously have escaped detection in folding studies based on pre-formed, full-length transcripts [ 22 , 33–35 ]. Kinetic studies of RNA folding have been primarily performed using T7 RNAP or E. coli RNAP.…”
Section: Introductionmentioning
confidence: 99%