The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway

Stührwohldt, Nils; Scholl, Stefan; Lang, Lisa; Katzenberger, Julia; Schumacher, Karin; Schaller, Andreas

doi:10.1101/483644

Cited by 3 publications

(5 citation statements)

References 64 publications

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“…Before GLVs are secreted and exert their physiological role, they require maturation through consecutive cleavage events by SBTs on the peptide precursors' passage through the secretory pathway. Pre-processing of GLV2 is executed by SBT6.1/S1P (Ghorbani et al, 2016;Stührwohldt et al, 2020). Intriguingly, S1P is additionally maturating RALF23 peptides which attenuate immunity by inhibiting the PRR scaffolding function of FER (Srivastava et al, 2008;Stegmann et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Similar results were obtained in at least 3 independent experiments. GLV2 requires sulfation at a conserved tyrosine residue by TPST to control meristematic activity and root gravitropism (Matsuzaki et al, 2010;Stührwohldt et al, 2020). The tpst mutants show increased immune responses which was mainly linked to PSK and PSY1-mediated immune regulation, both of which are also substrates of TPST (Igarashi et al, 2012;Mosher et al, 2013).…”

Section: Glv2 Peptides Increase Sensitivity Of Plants To Flg22 Percepmentioning

confidence: 99%

“…Fully maturated GLV peptides are usually 13-18 amino acids in length. The GLV1 and GLV2 peptide precursors are processed by SUBTILASE (SBT)6.1/SITE-1 PROTEASE (S1P) and SBT3.8 on their journey through the secretory pathway (Ghorbani et al, 2016;Stührwohldt et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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RGI-GOLVEN signalling promotes FLS2 abundance to regulate plant immunity

Stegmann

Zecua-Ramirez

Ludwig

et al. 2021

Preprint

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Endogenous plant signalling peptides regulate developmental and growth-related processes. Recent research indicates that some of these peptides are classified as phytocytokines as they have regulatory functions during plant immune responses. However, the mechanistic basis for phytocytokine-mediated immune modulation remains largely elusive. Here, we identify GOLVEN2 (GLV2) peptides as novel phytocytokines in Arabidopsis thaliana. By peptide application, precursor overexpression and loss-of-function studies we show that GLV2 enhances sensitivity of plants to elicitation with the bacterial flagellin epitope flg22. GLV2 is perceived by ROOT MERISTEM GROWTH FACTOR 1 INSENSITIVE (RGI) receptors and RGI3 forms an flg22-induced complex with the flg22-receptor FLAGELLIN SENSITIVE 2, suggesting that RGIs are part of activated pattern recognition receptor signalling platforms. GLV2 perception increases posttranscriptional FLS2 abundance and RGIs promote FLS2 protein accumulation. Thus, GLV-RGI signalling controls above ground plant immunity via a novel mechanism of phytocytokine activity.

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Section: Discussionmentioning

confidence: 99%

Section: Glv2 Peptides Increase Sensitivity Of Plants To Flg22 Percepmentioning

confidence: 99%

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RGI-GOLVEN signalling promotes FLS2 abundance to regulate plant immunity

Stegmann

Zecua-Ramirez

Ludwig

et al. 2021

Preprint

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“…Primers used to amplify sequences for the creation of new GreenGate entry vectors are given in Supplemental Table S3. GFP, mTurquoise and mCherry modules (pGGD011, pGGD-GSL-mTurquoise, pGGD010 and pGGF Alli-mCherry) were published before (Lupanga et al, 2020;Stührwohldt et al, 2020;Waadt et al, 2017;Stephani et al, 2020). Internal BsaI sites were mutated.…”

Section: Cloningmentioning

confidence: 99%

Networked proteins redundantly interact with VAP27 and RABG3 to regulate membrane tethering at the vacuole and beyond

Kaiser,

Mehlhorn,

Miranda

et al. 2023

Preprint

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Biological processes in eukaryotes depend on the spatio-temporal compartmentalization of their cells. Integrity and positioning of organelles on the other hand rely on the organization of the actin cytoskeleton. Previously, it has been shown that changes of the plants largest organelle, the vacuole, depends on a functional actin organization. The connection between actin filaments and the vacuole is established by the family of Networked (NET) 4 proteins and, consequently, altering NET4 abundance impacts vacuolar morphology. However, the precise regulatory mechanism is unknown and gene deletions ofNET4did not result in a global growth phenotype. Here we show that NET4 functions redundantly with NET3, interacting with RABG3-GTPases at the vacuole to allow for homotypic fusion or, alternatively, the generation of endoplasmic reticulum (ER) - vacuole contact sites. We found that ER-resident NET3 is able to interact with RABG3 residing at the tonoplast and that NET4 interacts with the contact site protein VAP27-1 at the ER. Generation ofnet3 net4triple mutants by CRSIPR-guided mutagenesis helped us to overcome functional redundancy, resulting in impaired plant growth and development. Our results demonstrate how diversification ofNETgenes led to functional redundancy between different family members to create cellular plasticity of vascular plants. We hypothesize that establishment of a direct ER-vacuole connection enables direct lipid and protein transfer which is especially important in young and fast-growing cells. Availability of lipids would facilitate rapidly expanding vacuoles which are the basis for high cell elongation rates and eventually fast plant growth.

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“…The signal peptide is cleaved in the ER to release the mature propeptide containing the variable domain and the C-terminal CLE peptide. The propeptide undergoes post-translational modification during its transit through the ER and Golgi apparatus, resulting, in particular, in a CLE motif decorated with hydroxyproline and arabinosyl residues (Matsubayashi, 2011;St€ uhrwohldt et al, 2020) (Fig. 1a).…”

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confidence: 99%