The biofunction of orange-spotted grouper (Epinephelus coioides) CC chemokine ligand 4 (CCL4) in innate and adaptive immunity

Hsu, Yi-Jiou; Hou, Chia-Yi; Lin, Shio Jean; Kuo, Wan-Ching; Lin, Han-Tso; Lin, John Han-You

doi:10.1016/j.fsi.2013.09.020

Cited by 34 publications

(15 citation statements)

References 54 publications

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“…The chemoattractant potential of two of them, Paol-SCYA104 [ 50 ] and JFCCL3 [ 51 ], has also been demonstrated after the production of the recombinant proteins using purified blood leukocytes. In orange-spotted grouper, a homologue to mammalian CCL4 was reported [ 53 ]. Recombinant CCL4 was found to have chemotactic activity for peripheral blood leukocytes [ 53 ], as well as the capacity to up-regulate the transcription of TNF-α1, TNF-α2, IFN-γ, Mx, T-bet and both CD8 α and β chains, suggesting that CCL4 not only attracts leukocytes, but also induces an inflammatory response and skews lymphocyte differentiation into the Th1 pathway.…”

Section: Identification Transcriptional Regulation and Functionalmentioning

confidence: 99%

Teleost Chemokines and Their Receptors

Bird

Tafalla²

2015

Biology

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Chemokines are a superfamily of cytokines that appeared about 650 million years ago, at the emergence of vertebrates, and are responsible for regulating cell migration under both inflammatory and physiological conditions. The first teleost chemokine gene was reported in rainbow trout in 1998. Since then, numerous chemokine genes have been identified in diverse fish species evidencing the great differences that exist among fish and mammalian chemokines, and within the different fish species, as a consequence of extensive intrachromosomal gene duplications and different infectious experiences. Subsequently, it has only been possible to establish clear homologies with mammalian chemokines in the case of some chemokines with well-conserved homeostatic roles, whereas the functionality of other chemokine genes will have to be independently addressed in each species. Despite this, functional studies have only been undertaken for a few of these chemokine genes. In this review, we describe the current state of knowledge of chemokine biology in teleost fish. We have mainly focused on those species for which more research efforts have been made in this subject, specifically zebrafish (Danio rerio), rainbow trout (Oncorhynchus mykiss) and catfish (Ictalurus punctatus), outlining which genes have been identified thus far, highlighting the most important aspects of their expression regulation and addressing any known aspects of their biological role in immunity. Finally, we summarise what is known about the chemokine receptors in teleosts and provide some analysis using recently available data to help characterise them more clearly.

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Section: Identification Transcriptional Regulation and Functionalmentioning

confidence: 99%

Teleost Chemokines and Their Receptors

Bird

Tafalla²

2015

Biology

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“…CC chemokine (motif) ligand 4 (CCL4) is indispensable for the chemoattraction of macrophages, natural killer cells, and lymphocytes in mammals. In LPS-stimulated fish, CCL4 expression did not increase until nearly 24 h after induction (Hsu et al, 2013). However, our results indicated that CCL4 was significantly upregulated in ducklings following treatment with LPS for 12 h ( Figure 2B).…”

Section: Real-time Pcr Confirmation Of Differential Gene Expression Imentioning

confidence: 63%

“…LBP is a crucial molecule involved in the innate immune response to a bacterial challenge (Jault et al, 2004;Barton and Kagan, 2009;Kawai and Akira, 2010;Ding et al, 2013, Hsu et al, 2013. These results indicate that the TLR signaling pathway participates in the immune response to Gram-negative bacterial infection in ducklings.…”

Section: Real-time Pcr Confirmation Of Differential Gene Expression Imentioning

confidence: 76%

Identification of genes and pathways related to lipopolysaccharide signaling in duckling spleens

Deng¹,

Yu²,

Liu³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Lipopolysaccharide (LPS), the major component of the outer cell wall of Gram-negative bacteria, activates the immune system and threatens the health of livestock and poultry. However, little is known about the genes and pathways involved in the immune response of ducklings to LPS. To elucidate the genes involved in the response of 7-day-old duckling spleens treated with LPS, RNA from LPS-treated and control duckling spleens was analyzed by RNA-Seq. The results showed 11,095 and 10,840 genes with >10 clean reads in the LPS-treated and control groups, respectively. Among these genes, 89 were differentially expressed (log 2 ratio ≥ 1, P ≤ 0.01, false discovery rate ≤ 0.001); 67 of these were upregulated and 22 were downregulated in the LPS-treated group compared to the control. GO and GO-rich analysis showed that differentially expressed genes were enriched in 13 functional categories (P < 0.05). Pathway analysis and pathway richness analysis showed that differentially expressed genes were enriched in six pathway categories (P < 0.05). Further analysis showed that some immune system-related signaling pathways, such as the hematopoietic cell lineage, Toll-like receptor signaling pathway, T cell receptor signaling pathway, T cell receptor signaling pathway, complement and coagulation cascades, antigen processing and presentation, and chemokine signaling pathway, are active during the immune response. To confirm the RNA-Seq results, we detected CCL4, LBP, CD71, and STEAP3 expression using real-time PCR analysis, and the results were consistent with the RNA-Seq results. Our results provide new information on the genes involved in the immune response of duckling spleens to LPS.

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“…The increase of IL-22 by Chlorella suggested that Chlorella might be involved in the regulation of fish anti-inflammatory and immunostimulatory functions. Chemokine (C-C motif) ligand 5 belongs to members of the Chlorella and immunity of gibel carp chemokines family and has been found to play a crucial role in the coordination of innate immunity (Hsu et al, 2013). Chorella could significantly increase CCL-5 levels in some tissues (P<0.05).…”

Section: Resultsmentioning

confidence: 99%

Effects of Dietary Administration of Chlorella on the Immune Status of Gibel Carp, Carassius Auratus Gibelio

Zhang

Qiu

et al. 2014

Italian Journal of Animal Science

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In the present study the effects of dietary Chlorella on the immune status of gibel carp, Carassius auratus gibelio, were evaluated. A total of 540 healthy fish were divided into 6 groups, with one control group fed with Chlorella-free diet and five experimental groups fed diets supplemented with 0.4 to 2.0% Chlorella. The whole trial lasted 60 days. At the end of the trial, the weight gain and immune parameters of fish were analysed. Results showed that Chlorella could increase the levels of immunoglobulin M and D, interleukin-22 and chemokine (C-C motif) ligand 5 in some tissues, which indicated that dietary Chlorella can be involved in regulating adaptive and innate immunity.

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The biofunction of orange-spotted grouper (Epinephelus coioides) CC chemokine ligand 4 (CCL4) in innate and adaptive immunity

Cited by 34 publications

References 54 publications

Teleost Chemokines and Their Receptors

Teleost Chemokines and Their Receptors

Identification of genes and pathways related to lipopolysaccharide signaling in duckling spleens

Effects of Dietary Administration of Chlorella on the Immune Status of Gibel Carp, Carassius Auratus Gibelio

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