The Biofilm Regulatory Network from Bacillus subtilis: A Structure-Function Analysis

Milton, Morgan E.; Cavanagh, John

doi:10.1016/j.jmb.2022.167923

Cited by 7 publications

(6 citation statements)

References 122 publications

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“…We used GeneMarkS v 56 software to predict protein coding Gene prediction of the whole gene sequence; PhiSpy was used to predict the presence of Prophage in the genome 57 ; IslandViewer 4 is used to predict the presence of gene islands in the genome 58 . We annotated the protein coding genes by searching KEGG databases 59 and NCBI nr, eggNOG, Swiss Prot, GO, TCDB, Pfam, CAZy , CARD, etc.…”

Section: Methodsmentioning

confidence: 99%

Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants

Shen,

Shi,

Zhao

et al. 2023

Sci Rep

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Bacillus is widely used in agriculture due to its diverse biological activities. We isolated a Bacillus velezensis SH-1471 from the rhizosphere soil of healthy tobacco, which has broad-spectrum antagonistic activity against a variety of plant pathogenic fungi such as Fusarium oxysporum, and can be colonized in the rhizosphere of a variety of plants. This study will further explore its mechanism by combining biological and molecular biology methods. SH-1471 contains a ring chromosome of 4,181,346 bp with a mean G + C content of 46.18%. We identified 14 homologous genes related to biosynthesis of resistant secondary metabolite, and three clusters encoded potential new antibacterial substances. It also contains a large number of genes from colonizing bacteria and genes related to plant bacterial interactions. It also contains genes related to environmental stress, as well as genes related to drug resistance. We also found that there are many metabolites in the strain that can inhibit the growth of pathogens. In addition, our indoor pot test found that SH-1471 has a good control effect on tomato wilt, and could significantly improve plant height, stem circumference, root length, root weight, and fresh weight and dry weight of the aboveground part of tomato seedlings. Therefore, SH-1471 is a potential biological control strain with important application value. The results of this study will help to further study the mechanism of SH-1471 in biological control of plant diseases and promote its application.

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Section: Methodsmentioning

confidence: 99%

Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants

Shen,

Shi,

Zhao

et al. 2023

Sci Rep

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“…Over the past three decades, several studies have been conducted on microbial biofilms of both pathogenic and non-pathogenic microorganisms. These studies have led to a significantly improved understanding of the underlying biochemical and molecular mechanisms of biofilm formation, maturation, and disintegration [16][17][18][19]. Several other studies have reported findings about identifying molecular targets enabling the discovery and development of potential antibiofilm therapeutics [20].…”

Section: Introductionmentioning

confidence: 99%

Simvastatin and Lovastatin inhibit Bacillus subtilis biofilm formation by interfering with the aggregation and amyloid formation of the TasA(28-261) –TapA(33-253) complex.

Verma,

Bajia,

Yadav

et al. 2024

Preprint

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Statins have been reported for diverse pleiotropic activities, including antimicrobial and antibiofilm. However, due to the limited understanding of their mode of action, none of the statins have gained approval for antimicrobial or antibiofilm applications. In a recent drug-repurposing study, we observed that two statins (i.e., Simvastatin and Lovastatin) interact stably with TasA(28-261), the principal extracellular matrix protein of Bacillus subtilis, leading to the inhibition of biofilm formation. Nevertheless, the precise mechanism underlying the biofilm inhibition remained elusive. In the present study, we examined the impact of these statins on the physiological activity of TasA(28-261, specifically its interaction with TapA(33-253) and aggregation into the amyloid-like structure using purified recombinant TasA(28-261) and TapA(33-253) in amyloid detection specific in vitro assays (i.e., CR binding & ThT staining assays). Results revealed that both statins interfered with amyloid formation by TasA(28-261)-TapA(33-253) complex, while neither statin inhibited amyloid formation by lysozyme, a model amyloid-forming protein. Moreover, neither statin significantly altered the expressions of terminal regulatory genes (viz., sinR, sinI) and effector genes (viz., tasA, tapA, and bslA) involved in biofilm formation by B. subtilis. These findings divulge that Simvastatin and Lovastatin inhibit biofilm formation by B. subtilis by interfering with the aggregation and amyloid formation by TasA(28-261)–TapA(33-253). These results represent one of the first experimental evidences for the underlying mechanism of antibiofilm activity of statins and offer valuable directions for future research to harness statins as antibiofilm therapeutics.

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“…Because of its robust biofilm-forming ability, this bacterial species is also an ideal model for biofilm research 16 . The molecular mechanisms of B. subtilis biofilm formation have been mostly investigated using two laboratory models: pellicles, which are floating biofilms at the airliquid interface, and colonies grown on an agar surface, which are architecturally intricate 3,17,18 .…”

Section: Bacillus Subtilis Is a Model Organism For A Variety Of Appli...mentioning

confidence: 99%

“…The core circuitry of biofilm regulation consists of two main protein categories, namely the global transition regulators Spo0A and AbrB, and the biofilm regulators SinR, SinI, and SlrR 16,61,62 . Spo0A plays a key role in regulating sporulation and is also the starting point of the biofilm formation process in B. subtilis 61 .…”

Section: Biofilm Matrix Composition In B Subtilismentioning

confidence: 99%

“…A phosphoryl group is then passed on to a conserved aspartate residue located in the N-terminal domain of Spo0A with the help of phosphotransferases, Spo0F and Spo0B 8,61 . Once the phosphoryl group is transferred to Spo0A, it becomes the active form of Spo0A called Spo0A˜P 16 . The aspartate residue in Spo0A can undergo both phosphorylation and dephosphorylation, thus affecting the activity of this master regulator 137 .…”

Section: Introductionmentioning

confidence: 99%

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The role and regulation of pulcherrimin during Bacillus subtilis biofilm development

Lima Angelini

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First and foremost, I would like to say thank you to all the members of my committee for their time and willingness to serve on it. I want to give a special thanks to my advisor, Dr. Yunrong (Win) Chai, for his guidance and great science discussions during my Ph.D. I am grateful that I was always allowed the freedom to be curious throughout my journey in graduate school. As a result of that, entire projects arose from a simple observation in the lab. I am grateful I made the choice to join the Chai lab! I would also like to extend my heartfelt thanks to Dr. Veronica Godoy-Carter for her joyful presence and helpful feedback during our meetings. I will always bring with me the high standards you made sure we kept, in the way we carry our research and ourselves as scientists. A huge thanks to Dr. Moshe Shemesh as well, for giving me such valuable science input and for being a great office desk neighbor. Your motivation and positivity towards science will always be an inspiration to me. Last but not least, I would also like to express my appreciation to Dr. Kim Lewis and Dr.Edward Geisinger for their invaluable contributions as members of my dissertation committee.Your constructive feedback based on your extensive scientific experiences were key in advancing my research.A huge thank you to Chai lab members, past and present, for their friendship, support, and guidance. A special thanks to the first Chai lab graduate, Dr. Jennifer Greenwich, for being one the most welcoming and friendly people I have ever met. Going out on Valentine's day with you at Fogo de Chão, as well as getting an entire red velvet cheesecake for my birthday, were one of the best memories I have had in grad school. Thank you to Dr. Cameron Habib, who I do not recall ever being introduced to, for his unlimited sarcastic jokes, Oliver pets, and for letting me inherit the best seat in the lab office. Thank you also to Yinghao He, who I have known since day 1 of Table of Contents

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The Biofilm Regulatory Network from Bacillus subtilis: A Structure-Function Analysis

Cited by 7 publications

References 122 publications

Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants

Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants

Simvastatin and Lovastatin inhibit Bacillus subtilis biofilm formation by interfering with the aggregation and amyloid formation of the TasA(28-261) –TapA(33-253) complex.

The role and regulation of pulcherrimin during Bacillus subtilis biofilm development

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