The biocompatibility and biofilm resistance of implant coatings based on hydrophilic polymer brushes conjugated with antimicrobial peptides

Gao, Guangzheng; Lange, Dirk; Hilpert, Kai; Kindrachuk, Jason; Zou, Yuquan; Cheng, John; Kazemzadeh-Narbat, Mehdi; Yu, Kai; Wang, Rizhi; Straus, Suzana K.; Brooks, Donald E.; Chew, Ben H.; Hancock, Robert E. W.; Kizhakkedathu, Jayachandran N.

doi:10.1016/j.biomaterials.2011.02.013

Cited by 360 publications

(333 citation statements)

References 43 publications

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“…This process was performed according to previous protocols reported by Gao et al 38,40 . In summary, the synthesis of copolymer brushes onto titanium surfaces involves the following steps: first, an atom transfer radical polymerization (ATRP) initiator is immobilized on the surfaces.…”

Section: Functionalization Of Titanium Samples Via Copolymer Brushes mentioning

confidence: 99%

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

et al. 2015

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Dental implant failure can be associated to infections which develop into periimplantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMP) have been studied. To covalently immobilize these molecules onto metallic substrates several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1-11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro.Smooth titanium samples were coated with hLf1-11 peptide under three different conditions: silanization with APTES, and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the non-fouling nature of polymer PDMA segment.3

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Section: Functionalization Of Titanium Samples Via Copolymer Brushes mentioning

confidence: 99%

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

et al. 2015

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“…The rationale, at this point, is to obtain functionalized surfaces with proven anti-adhesive and anti-biofilm traits to later be tested as potential harmless biomaterials. Several promising studies have already been made, namely with polymer brushes, which have the advantage of being robust and can be generated in most biomedical materials (Gao et al 2011a). Glinel et al (2012 report on the existing findings in this field, but further studies are required in order to escalate findings onto a broader range of biomedical surfaces and devices.…”

Section: Prophylactic Strategiesmentioning

confidence: 99%

New trends in peptide-based anti-biofilm strategies: a review of recent achievements and bioinformatic approaches

2012

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Antimicrobial peptides (AMPs) have a broad spectrum of activity and unspecific mechanisms of action. Therefore, they are seen as valid alternatives to overcome clinically relevant biofilms and reduce the chance of acquired resistance. This paper reviews AMPs and anti-biofilm AMP-based strategies and discusses ongoing and future work. Recent studies report successful AMP-based prophylactic and therapeutic strategies, several databases catalogue AMP information and analysis tools, and novel bioinformatics tools are supporting AMP discovery and design. However, most AMP studies are performed with planktonic cultures, and most studies on sessile cells test AMPs on growing rather than mature biofilms. Promising preliminary synergistic studies have to be consubstantiated and the study of functionalized coatings with AMPs must be further explored. Standardized operating protocols, to enforce the repeatability and reproducibility of AMP anti-biofilm tests, and automated means of screening and processing the ever-expanding literature are still missing.

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“…El inicio de la formación de la biopelícula, requiere de la adhesión de la bacteria a la superficie del implante ortopédico, seguido de la formación de microcolonias, las cuales desarrollarán una estructura de biopelícula multicapas (estado avanzado) 29 . Las propiedades de la superficie, principalmente la hidrofobicidad y la presencia de cargas, juegan un rol en la adhesión y proliferación bacteriana inicial 7 . La superficie del implante ortopédico, luego de ser colocada en el medio interno, acumula proteínas séricas y de la matriz extracelular (colágeno, fibronectina, fibrinógeno, vitronectina, sialoproteína ósea, elastina y trombospondina) que promueven la adherencia y colonización bacteriana, siendo éste un paso crítico en el desarrollo de las infecciones asociadas a implante ortopédico 5,29,31 .…”

Section: Infectología Al Díaunclassified

“…Sin embargo, la alteración de la superficie con tal de disminuir la infección es beneficiosa en el corto plazo, pero a largo plazo, puede llegar a alterar la integración del implante al tejido óseo 5 . El uso de antimicrobianos y recubrimientos son las dos técnicas más comúnmente usadas para prevenir la adhesión y difusión de microrganismos en las superficies 7 . El "patrón de oro" para liberación de antimicrobianos de forma local es el cemento óseo impregnado con antibacterianos (polimetilmetacrilato: PMMA).…”

Section: Estrategia De Prevenciónunclassified

“…Sin embargo, posee consecuencias devastadoras, determinando el retiro del implante y su sustitución en la mayoría de las veces, exponiendo al paciente a altos riesgos de recidiva infecciosa 1,5,6 . Es más, la infección de implantes ortopédicos resulta en una seguridad y longevidad alterada del mismo 7 . Actualmente, las tasas de infección notificadas en reemplazo primario de cadera y de rodilla son 0,3-0,6% y 1%, respectivamente.…”

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Bases moleculares de la infección asociada a implantes ortopédicos

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2014

Rev. chil. infectol.

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The biocompatibility and biofilm resistance of implant coatings based on hydrophilic polymer brushes conjugated with antimicrobial peptides

Cited by 360 publications

References 43 publications

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

New trends in peptide-based anti-biofilm strategies: a review of recent achievements and bioinformatic approaches

Bases moleculares de la infección asociada a implantes ortopédicos

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