The biochemistry of the regenerating liver

Lewan, Lillemor; Yngner, Torsten; Engelbrecht, Claes

doi:10.1016/0020-711x(77)90107-0

Cited by 52 publications

(20 citation statements)

References 109 publications

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“…They have highly differentiated hepatic functions, which are reduced when they enter a proliferative stage (Lewan et al, 1977;Nakamura et al, 1983b).…”

Section: Discussionmentioning

confidence: 97%

The control of DNA synthesis in primary cultures of hepatocytes from adult and young rats: Interactions of extracellular matrix components, epidermal growth factor, and the cell cycle

Tomomura

Sawada

Sattler

et al. 1987

Journal Cellular Physiology

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Hepatocytes from adult and 4-week-old rats cultured on one of several extracellular matrix components were stimulated to replicate by epidermal growth factor (EGF). DNA synthesis was increased at 44-48 hr in adult hepatocytes and at 24, 48, and 72 hr in hepatocytes from young rats when EGF was added 2 hr after explantation. When EGF was added at 24 hr, maximal DNA synthesis of adult hepatocytes was observed at 48 hr, whereas that of 4-week-old hepatocytes was seen at 48 and 72 hr. Ten ng EGF per ml was the optimal concentration for maximal DNA synthesis in both adult and young cells. DNA synthesis decreased with increasing cell density, but this effect was less in hepatocytes from young than in those from adults. When hepatocytes were cultured on substrata consisting of individual extracellular matrix components, neither the time that adult cells needed to respond to EGF nor the time from stimulation by EGF to the peak of maximal DNA synthesis was altered in either adult or young cells. The optimal EGF concentration for maximal DNA synthesis and the cell density control of replication were also not altered by the substrata used. Substrata made from each of the extracellular matrix components studied enhanced DNA synthesis of adult and young hepatocytes stimulated by EGF in the following decreasing order: fibronectin, type IV collagen, type I collagen, and laminin. In both adult and young hepatocytes the enhancement of DNA synthesis was greatest when cultured on fibronectin. Thus the initiation and magnitude of DNA synthesis in primary cultures of rat hepatocytes were altered both by the age of the donor and the substratum on which the cells were explanted.

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“…They have highly differentiated hepatic functions, which are reduced when they enter a proliferative stage (Lewan et al, 1977;Nakamura et al, 1983b).…”

Section: Discussionmentioning

confidence: 97%

The control of DNA synthesis in primary cultures of hepatocytes from adult and young rats: Interactions of extracellular matrix components, epidermal growth factor, and the cell cycle

Tomomura

Sawada

Sattler

et al. 1987

Journal Cellular Physiology

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“…Results are given in "absolute" terms, i.e. per lobe and not per unit of wet tissue, as in [9] and [10], or per unit protein [11] to avoid the effect of unbalanced fluctuations of the liver size. After hepatectomy the liver glycogen and lipid undergo remarkable changes (see, e.g., [6]), and the synthesis of various proteins [12] and the proliferation of cell and cell organelles (see, e.g., [6,11,13]) proceed at different rates.…”

Section: Introductionmentioning

confidence: 99%

Peroxisomal enzyme activities in regenerating liver of rats

Locci-Cubeddu

Bergamini

1982

Res. Exp. Med.

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The activities of five peroxisomal enzymes (fatty acyl-CoA oxidase, FAO; uricase, U; L-hydroxyacid oxidase, LHAO; D-aminoacid oxidase, DAO; and catalase, C and the triglyceride content were determined in the regenerating liver of rats after partial hepatectomy. The FAO activity was significantly increased on day 3. Thus, its rise followed the increase of the triglyceride content and preceded the disappearance of steatosis. For U and LHAO a similar progressive increase in activities was obtained on days 3, 5, and 7 of regeneration. DAO exhibited a highly significant increase on day 1. C increased on day 1 and then again after day 3. It is concluded that peroxisomal functions are not restored at parallel rates during liver regeneration.

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“…A detailed review of the biochemistry of this phenomenon has been recently published by Lewan et al [5]. It has been shown that quiescent liver cells of mammalians may be triggered to proliferate by removal of two-thirds of the organ.…”

mentioning

confidence: 98%

Enzyme activities in regenerating liver of rats

et al. 1980

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the activities of eight enzymes were determined in the regenerating liver of rats after partial hepatectomy. For five enzymes, i.e., aldolase, lactate dehydrogenase, alanine transaminase, 5-nucleotidase, and acid phosphatase a progressive statistically significant increase in activities was obtained on days 10 and 20 of regeneration. This increase was not at the high level observed for parallel activities in fetal liver of rats on the 20th day of gestation. A possible interpretation of the process of liver regeneration is suggested.

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The biochemistry of the regenerating liver

Cited by 52 publications

References 109 publications

The control of DNA synthesis in primary cultures of hepatocytes from adult and young rats: Interactions of extracellular matrix components, epidermal growth factor, and the cell cycle

The control of DNA synthesis in primary cultures of hepatocytes from adult and young rats: Interactions of extracellular matrix components, epidermal growth factor, and the cell cycle

Peroxisomal enzyme activities in regenerating liver of rats

Enzyme activities in regenerating liver of rats

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