The Biochemical Mode of Action of the Novel Selective Fungicide Cyazofamid: Specific Inhibition of Mitochondrial Complex III in Phythium spinosum

Mitani, Shigeru; Araki, Shigeru; Takii, Yasuko; Ohshima, T.; Matsuo, Norifusa; Miyoshi, Hideto

doi:10.1006/pest.2001.2569

Cited by 70 publications

(52 citation statements)

References 23 publications

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“…The mode of action of cyazofamid differs from that of other currently registered and commonly used fungicides. [9][10][11] Therefore, cyazofamid will become an excellent tool for use in fungicide resistance management strategies. 10,11) …”

Section: Resultsmentioning

confidence: 99%

Control of Potato Late Blight by Cyazofamid

2005

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Section: Resultsmentioning

confidence: 99%

Control of Potato Late Blight by Cyazofamid

2005

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“…Unfortunately, atovaquone-resistant parasites have been observed in the field following atovaquone or Malarone treatment failures (see, for example, references 2, 23, 38, and 41). Atovaquone, as do the fungicides and pesticides targeting the bc 1 complex, binds at the Q o site (the only exception is the fungicide cyazofamid that targets the Q i site and is only active against oomycetes [37]). Mutations in the Q o site have been reported that compromise the pathogen control (reviewed in reference 21).…”

Section: Discussionmentioning

confidence: 99%

HDQ, a Potent Inhibitor of Plasmodium falciparum Proliferation, Binds to the Quinone Reduction Site of the Cytochrome bc ₁ Complex

Vallières

Fisher

Antoine

et al. 2012

Antimicrob Agents Chemother

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“…17) Kinetic studies on the inhibition of UCR activity of B. cinerea by pyribencarb, kresoxim-methyl, azoxystrobin and antimycin A, suggested that pyribencarb did not bind to the same site as antimycin A, but bound in the neighborhood of kresoximmethyl and azoxystrobin. These results indicated that the binding site of pyribencarb existed on the o-center of the quinone cycle in cytochrome b in complex III.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of action and selectivity of a novel fungicide, pyribencarb

et al. 2010

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Pyribencarb is a novel benzylcarbamate-type fungicide, which is active against a wide range of plant pathogenic fungi. In this paper, the inhibitory effects of this fungicide on the electron transport system of fungi, plants, rat and carp were examined to elucidate its mode of action and selectivity. Pyribencarb potently inhibited succinatecytochrome c reductase (SCR) activities of Botrytis cinerea (cucumber gray mold), Corynespora cassiicola (leaf spot) and decylubiquinol-cytochrome c reductase (UCR) activity of B. cinerea. Pyribencarb inhibited the UCR of B. cinerea in an uncompetitive manner with respect to decylubiquinol, which was the same as strobilurin fungicides, and the substrate-dependent inhibition constant was found from calculation to be 13 nM. These results suggested that the target site of pyribencarb is cytochrome b of complex III in the electron transport system of the respiratory chain. On the other hand, the inhibitory potency of pyribencarb on SCR activities of plants, rats and carp was relatively weak compared with that of strobilurin fungicides, indicating that pyribencarb is a Qo inhibitor of cytochrome b, whose properties are superior to well-known Qo inhibitor fungicides in terms of target. The binding site of pyribencarb on cytochrome b was assumed to be a little different from that of strobilurin fungicides, because pyribencarb inhibited SCRs of strobilurin fungicide-resistant strains of B. cinerea and C. cassiicola with relatively low concentrations. The binding site was also discussed through comparison of amino acid sequences of plants, rats, carp, yeast and fungi, including B. cinerea, which was elucidated in this paper.

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The Biochemical Mode of Action of the Novel Selective Fungicide Cyazofamid: Specific Inhibition of Mitochondrial Complex III in Phythium spinosum

Cited by 70 publications

References 23 publications

Control of Potato Late Blight by Cyazofamid

Control of Potato Late Blight by Cyazofamid

HDQ, a Potent Inhibitor of Plasmodium falciparum Proliferation, Binds to the Quinone Reduction Site of the Cytochrome bc ₁ Complex

Mechanism of action and selectivity of a novel fungicide, pyribencarb

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The Biochemical Mode of Action of the Novel Selective Fungicide Cyazofamid: Specific Inhibition of Mitochondrial Complex III in Phythium spinosum

Cited by 70 publications

References 23 publications

Control of Potato Late Blight by Cyazofamid

Control of Potato Late Blight by Cyazofamid

HDQ, a Potent Inhibitor of Plasmodium falciparum Proliferation, Binds to the Quinone Reduction Site of the Cytochrome bc 1 Complex

Mechanism of action and selectivity of a novel fungicide, pyribencarb

Contact Info

Product

Resources

About

HDQ, a Potent Inhibitor of Plasmodium falciparum Proliferation, Binds to the Quinone Reduction Site of the Cytochrome bc ₁ Complex