The bioactivity of
            <i>Hedysarum coronarium</i>
            extracts on skin enzymes and cells correlates with phenolic content

Burlando, Bruno; Pastorino, Giulia; Salis, Annalisa; Damonte, Gianluca; Clericuzio, Marco; Cornara, Laura

doi:10.1080/13880209.2017.1346691

Cited by 19 publications

(27 citation statements)

References 21 publications

(25 reference statements)

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“…The total polyphenol content of PEE was determined by the Folin-Ciocalteu method as previously reported [ 29 ]. A standard curve was obtained from gallic acid samples and total polyphenols in PEE were expressed as mg GAE per g of dry extract.…”

Section: Methodsmentioning

confidence: 99%

“…Pre-adipocytes were grown as above, settled in 96-well plates, differentiated into adipocytes for one week in Adipocyte Differentiation Medium (cat# DM-2), and maintained for a further week in Adipocyte Medium (cat# AM-1). Fully differentiated adipocytes were incubated for 3 h with 10, 100, or 200 µg/mL PEE, and samples of conditioned medium were then assayed for glycerol as previously reported [ 29 ]. Samples were read in the microplate reader at 550 nm.…”

Section: Methodsmentioning

confidence: 99%

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Posidonia oceanica (L.) Delile Ethanolic Extract Modulates Cell Activities with Skin Health Applications

et al. 2018

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Seagrasses are high plants sharing adaptive metabolic features with both terrestrial plants and marine algae, resulting in a phytocomplex possibly endowed with interesting biological properties. The aim of this study is to evaluate the in vitro activities on skin cells of an ethanolic extract obtained from the leaves of Posidonia oceanica (L.) Delile, family Potamogetonaceae, herein named Posidonia ethanolic extract (PEE). PEE showed high radical scavenging activity, high phenolic content, and resulted rich in chicoric acid, as determined through HPLC-MS analysis. The use of MTT assay on fibroblasts showed a PEE cytotoxicity threshold (IC05) of 50 µg/mL at 48 h, while a sub-toxic dose of 20 µg/mL induced a significant increase of fibroblast growth rate after 10 days. In addition, an ELISA assay revealed that PEE doses of 5 and 10 µg/mL induced collagen production in fibroblasts. PEE induced dose-dependent mushroom tyrosinase inhibition, up to about 45% inhibition at 1000 µg/mL, while 50% reduction of melanin was observed in melanoma cells exposed to 50 µg/mL PEE. Finally, PEE lipolytic activity was assessed by measuring glycerol release from adipocytes following triglyceride degradation. In conclusion, we have collected new data about the biological activities of the phytocomplex of P. oceanica seagrass on skin cells. Our findings indicate that PEE could be profitably used in the development of products for skin aging, undesired hyperpigmentation, and cellulite.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Posidonia oceanica (L.) Delile Ethanolic Extract Modulates Cell Activities with Skin Health Applications

et al. 2018

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“…However, the most active plants showed a common pattern in their 1 H NMR spectra, indicating a high concentration of phenolic compounds. This general information was considered interesting since phenolic compounds are generally involved in enzyme inhibitory activities of plants and, in particular, in MMP inhibition [26,27]. The OPLS model revealed the correlation between the strength of collagenase inhibition and the signals in the aromatic region around δ 7.0 in the 1 H NMR spectra.…”

Section: Resultsmentioning

confidence: 96%

Identification of a Collagenase-Inhibiting Flavonoid from Alchemilla vulgaris Using NMR-Based Metabolomics

et al. 2018

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This paper describes the use of H NMR profiling and chemometrics in order to facilitate the selection of medicinal plants as potential sources of collagenase inhibitors. A total of 49 plants with reported ethnobotanical uses, such as the healing of wounds and burns, treatment of skin-related diseases, rheumatism, arthritis, and bone diseases, were initially chosen as potential candidates. The collagenase inhibitory activity of hydroalcoholic extracts of these plants was tested. Moreover, their phytochemical profiles were analyzed by H NMR and combined with the inhibitory activity data by an orthogonal partial least squares model. The results showed a correlation between the bioactivity and the concentration of phenolics, including flavonoids, phenylpropanoids, and tannins, in the extracts. Considering the eventual false-positive effect on the bioactivity given by tannins, a tannin removal procedure was performed on the most active extracts. After this procedure, was the most persistently active, proving to owe its activity to compounds other than tannins. Thus, this plant was selected as the most promising and further investigated through bioassay-guided fractionation, which resulted in the isolation of a flavonoid, quercetin-3---glucuronide, as confirmed by NMR and HRMS spectra. This compound showed not only a higher activity than other flavonoids with the same aglycone moiety, but was also higher than doxycycline (positive control), the only Federal Drug Administration-approved collagenase inhibitor. The approach employed in this study, namely the integration of metabolomics and bioactivity-guided fractionation, showed great potential as a tool for plant selection and identification of bioactive compounds in natural product research.

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“…High performance liquid chromatography, photocolorimetry, complexonometry and chromato spectrophotometry are most commonly used to quantify mangiferin content in plant raw materials as well as biological fluids. 6,39,40,51 The photocolorimetric definition of mangiferin in both the raw material and crystalline powder has a number of advantages, such as shortening the analysis steps and replacing the deficient vegetable, tetrahydrofuran, with dioxane. The method is based on mangiferin's ability to produce a complex compound with chlorine iron.…”

Section: Methodsmentioning

confidence: 99%

Pharmacognostical Investigations of Hedysarum Caucasicum Bieb. (Fabaceae) - An Ethnomedicinal Plant of Northern Caucasus, Russia, Determination of Mangiferin and Antibacterial Potentials

Serebryanaya¹,

R²,

A³

2020

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The genus Hedysarum L. combines about 285 species, which are predominantly common in Eurasia. The genus Hedysarum is distributed mainly in the Europe, but some species are also found in Asia. 44 H. caucasicum Bieb. grows in all highland areas of the North Caucasus, in the alpine zone, up to 3500 m. 10 This species is endemic, growing on the highland meadows of the Caucasus: in the Pre-Caucasus, Western and Eastern Transcaucasia, in Daghestan. It ABSTRACT The main active component of members of the genus Hedysarum is xanthone C-glycosidemangiferin which was found in the above-ground part of 17 species of Hedysarum. Mangiferin is contained in plants of the genus Hedysarum can serve as a chemotaxonomic marker of this genus, it has antiviral activity against DNA-containing viruses: Herpes simplex virus, Varicella zoster, Cytomegaloviruses, also has immunostimulatory properties (stimulates cellular and humoral immunity). We have prepared the morphological and anatomical studying, phytochemical research availability of tannines, flavonoids, xanthones, the free organic acids, the sugars and amino acids among which in the significant amount glutamic acid, aspartic acid and an arginine collect is established. The maintenance of the sum of xanthones made 7.12%. As a result of a research of amino-acid structure of a grass of Hedysarum caucasicum Bieb. presence at the significant amount of glutamic acid (13.58 g/kg), aspartic acid (13.61 g/kg), an arginine (14.99 g/kg) is revealed. In a grass of Hedysarum caucasicum Bieb. The quantitative maintenance of the sum of xanthones in terms of a specific indicator of a mangiferin is established. The technology of receiving a liquid extract by means of 80% of ethanol is developed, standardization is carried out it. It is established that extract of Hedysarum caucasicum Bieb. shows the antimicrobial activity concerning Shigella sonnei, Bacillus subtilis and B.anthracoides.

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The bioactivity of Hedysarum coronarium extracts on skin enzymes and cells correlates with phenolic content

Cited by 19 publications

References 21 publications

Posidonia oceanica (L.) Delile Ethanolic Extract Modulates Cell Activities with Skin Health Applications

Posidonia oceanica (L.) Delile Ethanolic Extract Modulates Cell Activities with Skin Health Applications

Identification of a Collagenase-Inhibiting Flavonoid from Alchemilla vulgaris Using NMR-Based Metabolomics

Pharmacognostical Investigations of Hedysarum Caucasicum Bieb. (Fabaceae) - An Ethnomedicinal Plant of Northern Caucasus, Russia, Determination of Mangiferin and Antibacterial Potentials

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