The bioactive efficiency of ultrasonic extracts from acorn leaves and green walnut husks against Bacillus cereus: a hybrid approach to PCA with the Taguchi method

Ozturk, Gokturk; Yetiman, Ahmet E.; Doğan, Mahmut

doi:10.1007/s11694-019-00041-7

Cited by 4 publications

(3 citation statements)

References 62 publications

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“…For this purpose, cell-free supernatant (CFS) was collected from MRS broth via centrifugation at 3500rpm for 15 min. DPPH scavenging activity was assessed according to Ozturk et al (2019) with some modifications. The reaction mixture was prepared by adding 2 mL freshly prepared 0.02mM DPPH onto 1 mL four times diluted CFS.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of metabolic and functional properties of cholesterol-reducing and GABA-producerLimosilactobacillus fermentumstrain AGA52 isolated from lactic acid fermented Shalgam by usingin vitroandin silicoprobiogenomic approaches

Yetiman

Horzum²,

Akbulut

2022

Preprint

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This study aimed to characterize the genomic and metabolic properties of a novel Lb. fermentum strain AGA52 which was isolated from a lactic acid fermented beverage called Shalgam. The genome size of AGA52 was 2,001,084 bp, which is predicted to carry 2,024 genes, including 50 tRNAs, 3 rRNAs, 3ncRNAs, 15 CRISPR repeats, 14 CRISPR spacers, and 1 CRISPR array. The genome has a GC content of 51.82% including 95 predicted pseudogenes, 56 complete or partial transposases and 2 intact prophages. The similarity of the clusters of orthologous groups (COG) was analysed by comparison with the other Lb. fermentum strains. The detected resistome on the genome of AGA52 was found to be intrinsic originated. Based on genome analyses many CAZYmes were identified that are responsible for carbohydrate degradation and adaptation to gastrointestinal conditions. Besides, it has been determined that AGA52 has an obligate heterofermentative carbohydrate metabolism due to the absence of the 1-phosphofructokinase (pfK) enzyme. Additionally, it is determined that the strain has a higher antioxidant capacity and is adaptable to gastrointestinal simulated environments. It was also observed that the AGA52 has antimicrobial activity against Y. Enterocolitica ATCC9610, B. cereus ATCC33019, S. enterica sv. Typhimurium, E.coli O157:h7 ATCC43897, L.monocytogenes ATCC7644, K.pneumoniae ATCC13883, and P. vulgaris ATCC8427. Cholesterol assimilation (33.9%) and GABA production capacities were also confirmed by in silico and in vitro. Overall, investigation of genomic and metabolic features of the AGA52 revealed that is a potential psychobiotic and probiotic dietary supplement candidate and can bring functional benefits to the host.

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Section: Methodsmentioning

confidence: 99%

Evaluation of metabolic and functional properties of cholesterol-reducing and GABA-producerLimosilactobacillus fermentumstrain AGA52 isolated from lactic acid fermented Shalgam by usingin vitroandin silicoprobiogenomic approaches

Yetiman

Horzum²,

Akbulut

2022

Preprint

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“…GWH has been recognized as a rich source of flavonoids, polyphenols, terpenoids, and quinones (Barekat et al., 2022). These compounds present in GWH have demonstrated potential benefits for human health, including anti‐inflammatory, analgesic, antibacterial, and antitumor properties (C. Chen et al., 2023; Ozturk et al., 2019). However, fresh GWH is highly prone to oxidative blackening due to its high moisture content, which is ∼90%.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the pulsed vacuum drying process of green walnut husk through temperature adaptive regulation

Ao,

Shen,

Cai

et al. 2023

Journal of Food Science

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This study aimed to optimize the temperature adaptive conditions of pulsed vacuum drying (PVD) for green walnut husk (GWH) to tackle the issues of severe environmental pollution and limited utilization of GWH. The results of the single‐factor experiment revealed that the optimal drying temperature for PVD of GWH was 65°C, with a pulsed ratio of 9 min: 3 min. The drying time decreased from 10.87 to 6.32 h with increasing drying temperature and from 8.83 to 6.23 kW·h/kg with increasing pulsed ratio. Energy consumption also decreased with shorter drying time and shorter vacuum time. Under this optimal variable temperature drying condition, GWH exhibited the highest total active substance content, with respective values of 9.43 mg/g for total triterpenes, 35.68 mg/g for flavonoids, 9.51 mg/g for polyphenols, and 9.55 mg/g for quinones. The experimental drying data of GWH were best fitted by a logarithmic model, with R2 values ranging from 0.9927 to 0.9943. Furthermore, the observed microstructure of GWH corresponded to the variations in total active substance content. This study provided valuable theoretical guidance for addressing environmental pollution associated with GWH and facilitating the industrialization and refinement of GWH drying processes.Practical ApplicationThere is a growing interest in harnessing the potential value of agricultural waste to transform low‐cost raw materials into high‐value products while mitigating environmental pollution. In this study, for the first time, the effects of variable temperature pulsed vacuum drying on the content of active substances, drying time, and energy consumption of green walnut husk (GWH) were investigated. The findings serve as a theoretical foundation for addressing environmental pollution issues associated with GWH and enabling the industrialization and precision drying of GWH.

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“…Because naphthoquinones with the characteristics of benzoquinones occur reduction reaction easily at higher temperatures, soxhlet extraction yielded relatively little JU [15]. But this procedure efficiency is relatively lower because of more solvents and energy as well as the irreversible adsorption of target components [16,17]. To increase extraction efficiency, the acknowledged approach of ultrasound-assisted extraction (UAE) is frequently used to extract plant active components [18][19][20].…”

mentioning

confidence: 99%

An integrated approach of homogenization‐ultrasound‐assisted extraction followed by high‐speed countercurrent chromatography for rapidly preparative separation naphthoquinones and diarylheptanes from Juglands mandshurica Maxim. exocarp

Chang,

Yu,

Yang

et al. 2023

J of Separation Science

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This research investigated the effectiveness of an integrated method for the extraction and separation of naphthoquinones and diarylheptanes from exocarp of Juglands mandshurica Maxim. (namely, green walnut husks). The target compounds were obtained by ultra‐turrax homogenization (UTH) coupled with ultrasound‐assisted extraction (UAE) technology followed by high‐speed countercurrent chromatography (HSCCC). The UTH‐UAE extraction method achieved higher efficiency with 2.49‐ and 2.36‐fold to those by UAE, and 1.39‐ and 1.34‐fold to those by UTH in a short time. HSCCC was adopted for further separation and purification; six target compounds, namely, regiolone (RE), juglone (JU), myricatomento‐genin (MG), galleon (GA), 2‐oxatrycyclo[13.2.2.13,7]eicosa‐3,5,7(20),15,17,18‐hexaen‐10‐16‐diol (OE), and juglanin A (JA), were separated with more than 95.37% purities and more than 84.71% final recovery rates, respectively. In this study, the integrated strategy of extraction and separation could get high purity compounds quickly, which would provide time and solvent saved method for the natural products separation from plants.

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The bioactive efficiency of ultrasonic extracts from acorn leaves and green walnut husks against Bacillus cereus: a hybrid approach to PCA with the Taguchi method

Cited by 4 publications

References 62 publications

Evaluation of metabolic and functional properties of cholesterol-reducing and GABA-producerLimosilactobacillus fermentumstrain AGA52 isolated from lactic acid fermented Shalgam by usingin vitroandin silicoprobiogenomic approaches

Evaluation of metabolic and functional properties of cholesterol-reducing and GABA-producerLimosilactobacillus fermentumstrain AGA52 isolated from lactic acid fermented Shalgam by usingin vitroandin silicoprobiogenomic approaches

Optimization of the pulsed vacuum drying process of green walnut husk through temperature adaptive regulation

An integrated approach of homogenization‐ultrasound‐assisted extraction followed by high‐speed countercurrent chromatography for rapidly preparative separation naphthoquinones and diarylheptanes from Juglands mandshurica Maxim. exocarp

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