The bioactive composite film prepared from bacterial cellulose and modified by hydrolyzed gelatin peptide

Abstract

The hydrolyzed gelatin peptides, obtained from the hydrolysis of Tilapia nilotica skin gelatin with alcalase and pronase E, were fractionated using an ultrafiltration system into hydrolyzed gelatin peptides-a (10 kDa membrane), hydrolyzed gelatin peptides-b1, and hydrolyzed gelatin peptides-b2 (5 kDa membrane) fractions. The highest oxygen radical absorbance capacity was observed in hydrolyzed gelatin peptides-b2, which contained more nonpolar amino acids than the other hydrolyzed gelatin peptides. Hydrolyzed gelatin peptides-b2 at a concentration of 12.5 mg/ml exhibited the highest proliferation ability and increased the expression of Type I procollagen mRNA, which indicated an enhanced collagen synthesis. Hydrolyzed gelatin peptides protected Detroit 551 cells from 2,2'-azobis(2-amidinopropane) dihydrochloride-induced oxidative damage and increased cell viability. Hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm were less eligible for Detroit 551 cell proliferation than bacterial cellulose. The release of hydrolyzed gelatin peptides in bacterial cellulose film was slower than that in hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm; thus, bacterial cellulose film and hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm are suitable candidates for applications in delayed release type and rapid release type biofilms, respectively.

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

“…Cellulose derivatives, such as CMC and MC, ethyl cellulose, acetyl cellulose, and hydroxypropyl cellulose have frequently been used to formulate hydrogels, nanoparticles (e.g., nanowhiskers), and nanofibers . Moreover, in combination with other synthetic and natural polymers, such as proteins, in particular gelatin, a wide range of applications have been demonstrated for these composite materials as scaffolds for 3D cellular engineering . Glycosaminoglycans (GAGs) in the ECM may be mimicked by electrospinning partially sulfated cellulose with gelatin, yielding functional fibrous structures.…”

Hurdle Effect of Antimicrobial Activity Achieved by Time Differential Releasing of Nisin and Chitosan Hydrolysates from Bacterial Cellulose

“…Nguyen and others () reported that nisin could be released from BC within 6 h. Moreover, Huang and others () supplemented HPMC into a BC fermentation culture medium to disrupt the in situ formation of the BC structure and create HBC. HBC is a suitable candidate for controlled‐release applications because the reduced packed array of cellulose ribbons in HBC may increase the available space for diffusing water molecules and low molecular substances, generating an increased release of compounds from cellulose networks in HBC (Lin and others ). Therefore, we chose HBC to regulate the release and combination of nisin and CHs.…”

“…Huang and others () added hydroxypropylmethylcellulose (HPMC) into a BC fermentation culture medium to disrupt the in situ formation of the BC structure and created a HPMC‐modified BC (HBC). HBC showed lower crystallinity than BC did as well as improvement in water and small molecule absorption (Huang and others ) and controlled‐release activity (Lin and others ).…”