The Bioactivated Interfacial Behavior of the Fluoridated Hydroxyapatite-Coated Mg-Zn Alloy in Cell Culture Environments

Abstract

A partially fluorine substituted hydroxyapatite- (FHA-) coated Mg-Zn alloy was prepared to investigate the interfacial behavior of degradable Mg-based biomaterials with degradable bioactive coatings in a cell culture environment. Peaks from the results of X-ray diffraction (XRD) were characterized and compared before and after cell culture. It was found that Ca-P, including poorly crystalline ion-substituted Ca-deficient HA (CDHA), was formed in greater amounts on the interface of coated samples compared with the uncoated ones. A thermodynamic mechanism for Ca-P formation on biodegradable Mg alloys in a cell culture environment is proposed. Combined with improved cell calcification, the-FHA coated Mg alloys have the ability to promote CDHA formation, as expected thermodynamically. It is suggested that the specific cell culture environment and the bone-like FHA coatings together facilitate the observed behavior. Moreover, cell culture environment probably increased the biomineralization to a detectable level by affecting the kinetics of apatite formation.

Influences of Extrusion and Silver Content on the Degradation of Mg‐Ag Alloys In Vitro and In Vivo

“…The existence of C implied the deposition or absorption of organic matter. The deposition of Ca and P indicated the formation of calcium phosphates on the alloy surface, which plays an important role in biomineralization [ 26 , 29 – 31 ]. The Ca/P ratio was calculated for each position to estimate the calcium phosphate formation process.…”

Characterization and In Vitro and In Vivo Assessment of a Novel Cellulose Acetate-Coated Mg-Based Alloy for Orthopedic Applications

“…Studies have shown that Mg-Ca, Mg-Zn and Mg-Mn-Zn alloys demonstrated good in vitro and in vivo biocompatibility and enhanced corrosion resistance, dissolving progressively within the bone tissue [ 5 , 6 , 7 ]. Likewise, surface modifications through applying different coatings on Mg or Mg alloys (such as hydroxyapatite (HA: Ca 10 (PO 4 ) 6 (OH) 2 ) on Mg-Zn [ 8 , 9 ], HA–chitosan on AZ31 [ 10 , 11 ], bioglass [ 12 ] or β-TCP on AZ31 [ 13 , 14 ], etc.) proved to efficiently slow down the degradation process of Mg-based biomaterials and to diminish the hydrogen evolution.…”