The binding of cationic surfactants by DNA

Hayakawa, Kou; Santerre, J. Paul; Kwak, Jan C. T.

doi:10.1016/0301-4622(83)87001-x

Cited by 179 publications

(147 citation statements)

References 32 publications

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“…Binding of cationic surfactant to DNA occurs at concentrations well below the CMC of the surfactant and the binding isotherms have a sigmoidal shape which demonstrates the cooperative binding [16][17][18][19]. The binding isotherms were shown to be strongly dependent on the surfactant chain length [16], suggesting that hydrophobic interactions are important for the interaction and that it was analogous to the formation of micelles.…”

Section: Introductionmentioning

confidence: 95%

DNA and surfactants in bulk and at interfaces

Dias

Pais

Miguel

et al. 2004

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Recent investigations of the DNA interactions with cationic surfactants and catanionic mixtures are reviewed. Several techniques have been used such as fluorescence microscopy, dynamic light scattering, electron microscopy, and Monte Carlo simulations.The conformational behaviour of large DNA molecules in the presence of cationic surfactant was followed by fluorescence microscopy and also by dynamic light scattering. These techniques were in good agreement and it was possible to observe a discrete transition from extended coils to collapsed globules and their coexistence for intermediate amphiphile concentrations. The dependence on the surfactant alkyl chain was also monitored by fluorescence microscopy and, as expected, lower concentrations of the more hydrophobic surfactant were required to induce DNA compaction, although an excess of positive charges was still required.Monte Carlo simulations on the compaction of a medium size polyanion with shorter polycations were performed. The polyanion chain suffers a sudden collapse as a function of the concentration of condensing agent, and of the number of charges on the polycation molecules. Further increase in the concentration increases the degree of compaction. The compaction was found to be associated with the polycations promoting bridging between different sites of the polyanion. When the total charge of the polycations was lower than that of the polyanion, a significant translational motion of the compacting agent along the polyanion was observed, producing only a small-degree of intrachain segregation, which can explain the excess of positive charges necessary to compact DNA.Dissociation of the DNA-cationic surfactant complexes and a concomitant release of DNA was achieved by addition of anionic surfactants. The unfolding of DNA molecules, previously compacted with cationic surfactant, was shown to be strongly dependent on the anionic surfactant chain length; lower amounts of a longer chain surfactant were needed to release DNA into solution. On the other hand, no dependence on the hydrophobicity of the compacting agent was observed. The structures of the aggregates formed by the two surfactants, after the interaction with DNA, were imaged by cryogenic transmission electron microscopy. It is possible to predict the structure of the aggregates formed by the surfactants, like vesicles, from the phase behaviour of the mixed surfactant systems.Studies on the interactions between DNA and catanionic mixtures were also performed. It was observed that DNA does not interact with negatively charged vesicles, even though they carry positive amphiphiles; however, in the presence of positively charged vesicles, DNA molecules compact and adsorb on their surface.Finally Monte Carlo simulations were performed on the adsorption of a polyelectrolyte on catanionic surfaces. It was observed that the mobile charges in the surface react to the presence of the polyelectrolyte enabling a strong degree of adsorption even though the membrane was globally neutral. Our observati...

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Section: Introductionmentioning

confidence: 95%

DNA and surfactants in bulk and at interfaces

Dias

Pais

Miguel

et al. 2004

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…6,7 In bulk, the binding of cationic surfactants to DNA appears to be analogous to binding of surfactants to oppositely charged synthetic polymers in general. 1 The DNA molecule, however, has a special structure compared to typical synthetic polymers. In water solutions of low ionic strength, DNA is a rodlike and highly negatively charged polymer with a persistence length of more then 50 nm.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5] This is due to the large and increasing interest for using cationic liposomes as a possible way for in vivo gene transfer. 6,7 In bulk, the binding of cationic surfactants to DNA appears to be analogous to binding of surfactants to oppositely charged synthetic polymers in general.…”

Section: Introductionmentioning

confidence: 99%

“…8 The cooperative binding of surfactants is believed to be a result of micellar-like aggregation on the DNA chain. 1,[8][9][10] The rigidity of the DNA locally prevents the DNA chain to wrap itself around the surfactant aggregates. For short DNA chains (i.e., low molecular weight DNA) the DNA chain is too short and rigid to loop back to interact with surfactant aggregates on other parts of the polymer chain.…”

Section: Introductionmentioning

confidence: 99%

“…The binding of cationic surfactants proceeds in two stages. 1,[8][9][10] In the first stage, surfactants exchange with counterions condensed at the DNA chain. At a critical surfactant concentration a highly cooperative binding of surfactant occurs which is typically followed by phase separation.…”

Section: Introductionmentioning

confidence: 99%

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DNA−Surfactant Complexes at Solid Surfaces

et al. 2001

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In this work, we report on the adsorption of complexes between DNA of different molecular weight and a cationic surfactant, cetyltrimethylammonium bromide (CTAB), on hydrophobized and hydrophilic negatively charged silica surfaces as measured by ellipsometry. We will demonstrate how the adsorption is affected by the state of the DNA-surfactant complexes formed in bulk solution. High molecular weight DNA molecules, which condense (transform from coil to globule state) on addition of small amounts of cationic surfactants, do not adsorb on hydrophilic silica prior to phase separation. However, DNA-surfactant complexes formed from low molecular weight DNA were found to adsorb. For these complexes surfactants interact with DNA, without condensation of the DNA. Adsorbed DNA-surfactant complexes can easily be removed from the hydrophilic silica surface when replacing the bulk DNA-surfactant solution with pure salt solution. At the hydrophobic surface the DNA adsorbs without addition of cationic surfactant. However, with addition of a very low amount of surfactant, a rapid increase in adsorbed amount and a simultaneous decrease in adsorbed layer thickness are observed. This compaction of the adsorbed layer is to some extent reversible when replacing the bulk DNA-surfactant solution with pure salt solution.

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Changes in the polyelectrolyte-amphiphile interaction due to helix-coil transition induced by specific counterions or variations in temperature

Caram-Lelham

Sundelöf

1998

Biopolymers

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The binding of cationic surfactants by DNA

Cited by 179 publications

References 32 publications

DNA and surfactants in bulk and at interfaces

DNA and surfactants in bulk and at interfaces

DNA−Surfactant Complexes at Solid Surfaces

Changes in the polyelectrolyte-amphiphile interaction due to helix-coil transition induced by specific counterions or variations in temperature

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