The Binding, Internalization, and Release of Thymocytes by Thymic Nurse Cells

Philp, Deborah; Pezzano, Mark; Li, Yang; Omene, Coral; Boto, William; Guyden, J.

doi:10.1006/cimm.1993.1114

Cited by 24 publications

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“…7). TEM of the multicellular complex that resulted from thymocyte uptake produced images identical to those detected in freshly isolated TNCs (32,42). That is, thymocytes appeared to be isolated within membrane vacuoles.…”

Section: Thymocyte Internalization By Tncsmentioning

confidence: 78%

“…Cells from another nurse cell line, termed B/c TEC-L1 (36), were shown to take up PNA ϩ lymphocytes in culture. PNA is expressed exclusively on triple-positive thymocytes (42). Complete internalization of thymocytes by B/c TEC-L1 cells was not described.…”

Section: Thymocyte Internalization By Tncsmentioning

confidence: 99%

“…Data obtained from TEM and antibody-staining experiments have been presented to show multicellular complexes resulting from TNC internalization of added thymocytes. The internalization event has also been visualized using long-term video microscopy (42). In this presentation, internalization is defined as membrane sealed or complete separation from the extracellular environment.…”

Section: Structure Of Tncsmentioning

confidence: 99%

“…The internalization of thymocytes by TNCs was captured through the addition of freshly isolated thymocytes to cultures containing cells derived from the immortalization of TNCs (20, (42). Freshly isolated thymocytes were added to monolayers of SVT-II2 cells and incubated overnight.…”

Section: Thymocyte Internalization By Tncsmentioning

confidence: 99%

“…Long-term video microscopy was used to document the internalization event in SVT-II2 cells, one of the SV40-derived lines (42). Monolayers of SVT-II2 cells were exposed to freshly isolated thymocytes.…”

Section: Thymocyte Internalization By Tncsmentioning

confidence: 99%

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Questionable Thymic Nurse Cell

Pezzano

Samms

Martinez

et al. 2001

Microbiol Mol Biol Rev

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Since their discovery in 1980, thymic nurse cells (TNCs) have been controversial. Questions pertaining to the existence of the TNC as a “unit” cell with thymocytes completely enclosed within its cytoplasm were the focus of initial debates. Early skeptics proposed the multicellular complex to be an artifact of the procedures used to isolate TNCs from the thymus. Since that time, TNCs have been found in fish, frogs, tadpoles, chickens, sheep, pigs, rats, mice, and humans. Their evolutionary conservation throughout the animal kingdom relieved most speculations about the existence of TNCs and at the same time demonstrated their apparent importance to the thymus and T-cell development. In this review we will discuss and debate reports that describe (i) the organization or structure of TNCs, (ii) the thymocyte subset(s) found within the cytoplasm of TNCs and their uptake and release, and (iii) the function of this fascinating multicellular interaction that occurs during the process of T-cell development. Discussions about the future of the field and experimental approaches that will lead to answers to remaining questions are also presented

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Section: Thymocyte Internalization By Tncsmentioning

confidence: 78%

Section: Thymocyte Internalization By Tncsmentioning

confidence: 99%

Section: Structure Of Tncsmentioning

confidence: 99%

Section: Thymocyte Internalization By Tncsmentioning

confidence: 99%

Section: Thymocyte Internalization By Tncsmentioning

confidence: 99%

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Questionable Thymic Nurse Cell

Pezzano

Samms

Martinez

et al. 2001

Microbiol Mol Biol Rev

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Thymic nurse cells: Their functional ultrastructure

Brelińska¹,

Warchoł

1997

Microsc. Res. Tech.

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Thymic nurse cells are defined in vitro as multicellular complexes of epithelial cells and thymocytes. Although these structures have been implicated in the intrathymic differentiation of thymocytes, little is known about the biology of this cell complex and about the occurrence of the cells in the thymus in situ. Therefore, to clarify the matter, in this review we have presented characteristics of epithelial cells capable of forming complexes with thymocytes, in light of the literature data and the experience of the authors. The structure of cells within the complexes allowed us to distinguish three types of thymic nurse cells. Three‐dimensional reconstruction of the thymus and observations employing TEM and SEM demonstrated the presence of distinct types of complexes in various topographic regions of the thymus. Where possible, the functional relevance of the morphological data was analyzed. Microsc. Res. Tech. 38:250–266, 1997. © 1997 Wiley‐Liss, Inc.

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The Thymic Niche and Thymopoiesis

Chidgey

Boyd

Hugo

2007

Encyclopedia of Life Sciences

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The thymus, a bilobate gland located in the upper anterior thorax in higher vertebrates, is the major site of T‐lymphocyte production. This primary lymphoid organ composed of various nonlymphoid elements, referred to as the thymic stroma, governs the maturation and tolerance induction of haematopoietic precursors into immune‐competent nonautoreactive T cells. Whilst naturally involuting with age, the thymic stromal compartment remains dynamic in nature throughout life and retains the potential to regenerate.

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The Binding, Internalization, and Release of Thymocytes by Thymic Nurse Cells

Cited by 24 publications

References 0 publications

Questionable Thymic Nurse Cell

Questionable Thymic Nurse Cell

Thymic nurse cells: Their functional ultrastructure

The Thymic Niche and Thymopoiesis

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