The binding components for 2,3,7,8-tetrachlorodibenzo-p-dioxin and polycyclic aromatic hydrocarbons. Separation from the rat and mouse hepatic cytosol and characterization of a light density component.

Lesca, P.; Fernández, Nadia; Roy, Marianne

doi:10.1016/s0021-9258(18)61271-2

Journal of Biological Chemistry

1987

DOI: 10.1016/s0021-9258(18)61271-2

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The binding components for 2,3,7,8-tetrachlorodibenzo-p-dioxin and polycyclic aromatic hydrocarbons. Separation from the rat and mouse hepatic cytosol and characterization of a light density component.

P. Lesca¹,

Nadia Fernández²,

Marianne Roy³

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Cited by 36 publications

(1 citation statement)

References 29 publications

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“…The association of drugs with blood components including lipoproteins has gained much interest during the last decade. Many lipophilic drugs appear to show a tendency to bind to serum lipoproteins: cyclosporin A (Lemaire & Tillement, 1982), polychlorobiphenyls (Lesca et al, 1987), steroids (Hobbelen et al, 1975) and benzo[a]pyrene (Shu & Nichols, 1979) are among the many compounds reported to bind to these blood components.…”

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Properties of incorporation, redistribution, and integrity of porphyrin-low-density lipoprotein complexes

Pc¹,

Aj²,

Tj³

1993

Biochemistry

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In the photodynamic therapy of cancer, research has focused on the influence of lipoproteins (particularly the low-density lipoprotein, LDL) on the fate and transport of the porphyrin mixture. We have studied the interaction between LDL and a series of well-defined tetraphenylporphinesulfonates, TPPS-1, TPPS-2A, and TPPS-4. Compounds with at least two unsulfonated phenyl groups were found to associate with LDL (TPPS-1 and TPPS-2A), whereas sulfonation of all four rings abolished lipoprotein binding. As shown with agarose gel electrophoresis, association of doubly charged TPPS-2A molecules with LDL strongly influences LDL's charge. Because a change in charge may alter LDL's biological behavior, the effect of increasing amounts of TPPS-2A molecules per LDL on its biological reactivity was examined. In vitro studies with Hep G2 cells indicated that up to 250 molecules of TPPS-2A per LDL left LDL receptor recognition unchanged. In vivo studies on the fate of 125I-LDL-TPPS-2A particles in rats showed that complexes with molar ratios up to 1:100 were processed like native LDL. It is concluded that tetraphenylporphines of a partial hydrophilic-hydrophobic nature are most optimal for spontaneous association with lipoproteins. These porphyrin structures will utilize lipoproteins as biological transport vehicles whereby up to 100 molecules per lipoprotein particle will not change the biological behavior of the particles so that LDL receptor-dependent uptake by tumor cells under these conditions is warranted.

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mentioning

confidence: 99%

Properties of incorporation, redistribution, and integrity of porphyrin-low-density lipoprotein complexes

Pc¹,

Aj²,

Tj³

1993

Biochemistry

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Kinetics and conductivity parameters of uptake and transport of polychlorinated biphenyls in the Caco‐2 intestinal cell line model

Dulfer

Govers

Groten

1998

Enviro Toxic and Chemistry

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Abstract-Most of the accumulation of polychlorinated biphenyls (PCBs) over the food chain can be attributed to contaminant uptake from food. The effect of fatty acid absorption on net uptake and transport fluxes of a selection of 14 PCBs over the organismal gut epithelium has been determined in monolayers of the colon carcinoma cell line Caco-2 as a model system. The thermodynamic fugacity approach and the physiology of enterocyte lipid digestion are combined to investigate gastrointestinal absorption of polychlorinated biphenyls. The resistances of the subsequent transport steps from the apical compartment through the enterocytes to the basolateral compartment were quantified in terms of conductivity parameters. During uptake of PCBs into the enterocytes, the resistance of the aqueous diffusion barrier adjacent to the brush-border membrane is overcome for more than 99% by means of micellar transport. In the interior of the cells, the aquatic cytoplasm compartment also provides an important route of transport to the PCBs with up to three chlorine atoms, but the more hydrophobic PCBs are preferentially transported by triglyceride particles and lipoproteins. Calculated triolein/water partition coefficients, K tri , in general are higher than taurocholate micelle/water, dimyristoylphosphatidylcholine membrane/water and n-octanol/water partition coefficients, K TC , K DMPC , and K ow , respectively. Calculations suggest that the correlation between log K ow and log K tri is nonlinear.

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Identification and immunochemical characterization of IgA in sera of patients with mammary tumors

Chagnaud

Faiderbe

Geffard

1992

Intl Journal of Cancer

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It has been reported that several types of human cancer are associated with elevated levels of class-A immunoglobulins (Ig) and IgA-containing immune complexes. Moreover, most previous work has come up against the lack of IgA reactivity for chemically defined antigen (Ag). To overcome this, we first evaluated possible immunological binding in sera of patients with mammary tumors or malignant hematologic diseases and controls on a mouse monoclonal anti-idiotypic antibody (Ab2), internal image of conjugated benzo(a)pyrene (BP) coated on well plates. Using this indirect ELISA, a statistically highly significant immunological binding was found in sera of patients with mammary tumors of every grade, type and size. This immunological binding was linked to the IgA isotype. Second, we performed competition experiments between a BP conjugate coated on well-plates and anti-anti-"BP-like" antibodies (Ab) previously incubated with rabbit idiotypic antibodies (Ab1) directed against conjugated BP. A part of these anti-anti-BP-like Ab, raised in rabbits immunized with human IgA of patients with mammary tumors, recognized the Ag-combining site of polyclonal Ab1, previously developed in a rabbit immunized with BP conjugates. It appears that part of the human Ig from sera of patients with mammary tumors shares common idiotopes with rabbit polyclonal Ab1 raised against conjugated BP.

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The binding components for 2,3,7,8-tetrachlorodibenzo-p-dioxin and polycyclic aromatic hydrocarbons. Separation from the rat and mouse hepatic cytosol and characterization of a light density component.

Cited by 36 publications

References 29 publications

Properties of incorporation, redistribution, and integrity of porphyrin-low-density lipoprotein complexes

Properties of incorporation, redistribution, and integrity of porphyrin-low-density lipoprotein complexes

Kinetics and conductivity parameters of uptake and transport of polychlorinated biphenyls in the Caco‐2 intestinal cell line model

Identification and immunochemical characterization of IgA in sera of patients with mammary tumors

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