The Biliprotein C-Phycocyanin Modulates the DNA Damage Response in Lymphocytes from Nuclear Power Plant Workers

Role of Spirulina on gamma-irradiated rats using Fourier transform infrared attenuated total reflectance and Electron spin resonance for brain

“…This result of the absorbance of DNA/RNA is in accordance with (Klimova & Misurova, 2002), who found that in the cerebellum of irradiated rats (3 Gy) an increase in DNA concentration. Spirulina given before or after γ-irradiation ameliorates the increase in DNA, which is in accordance with (Stankova et al, 2011). Their finding suggested that the biliprotein could selectively boost the DNA repair ability of individuals with higher radiosensitivity, which may be of particular interest to the radioprotection of high-risk population and staff subgroups.…”

Proteasome inhibition protects human peripheral blood mononuclear cells from radiation-induced oxidative stress

“…For each experiment, cells from 3 -5 donors were pooled right after the isolation and then split into four samples ( ~ 2 ϫ 10 6 cells/ml), suspended in RPMI (Roswell Park Memorial Institute, Sigma-Aldrich, St Louis, MO, USA) 1640 medium, supplemented with 10% fetal calf serum (Sigma-Aldrich), and subjected to four diff erent treatments at room temperature (Ivanova et al 2010, Stankova et al 2011): (i) 4 h of incubation with RPMI before lysis; (ii) 2 h of incubation in RPMI, followed by in vitro irradiation with diff erent doses ranging from 0.5 -4 Gy (dose rate 2.07 Gy/min, 137 Cs gamma source, Gitawa Ltd, Sofi a, Bulgaria), incubation for another 2 h, and lysis; (iii) 4 h of incubation with 0.1 μ M MG132, 0.01 or 0.1 μ M lactacystin and 0.1 or 1 μ M celastrol in RPMI before lysis; and (iv) 2 h of incubation with the corresponding inhibitor, followed by irradiation with diff erent doses (from 0.5 -4 Gy), incubation for another 2 h and lysis. Th e three compounds, MG132, lactacystin and celastrol, were products of EMD Millipore, Darmstadt, Germany.…”

HSP90 Inhibitor Geldanamycin as a Radiation Response Modificator in Human Blood Cells

“…PBMC were isolated from 3-5 ml of venous blood from healthy volunteer donors. Isolation was performed by density gradient, as previously described ( Stankova et al . 2011 , Ivanova et al .…”