The bifidogenic effects and dental plaque deformation of non-digestible isomaltooligosaccharides synthesized by dextransucrase and alternansucrase

Nguyen, Thi Thanh Hanh; Lee, Donggu; Apu, Md. Aminul Islam; Jung, Jong‐Hyun; Kim, Minkyu; Lim, Sangyong; Chung, Byoungsang; Pal, Kunal; Kim, Doman

doi:10.1016/j.enzmictec.2021.109955

Cited by 6 publications

(1 citation statement)

References 43 publications

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“…Functional properties of IMOs on human and animal health are well recognized by many studies. IMOs selectively stimulate the growth or activity of beneficial bacteria or probiotics, in particular Bifidobacterium and Lactobacillus [ 7 , 8 ], promote peristalsis, prevent and relieve constipation and diarrhea [ 9 ] and prevent dental plaque [ 10 ]. Due to the stimulation of Bifidobacterium , Lactobacillus and other probiotics, IMOs indirectly affect various health prevention functions, including anti-tumor function, protection of the liver, improvement of the immune system, reduction in total serum cholesterol and triglycerides and prevention of cardiovascular and cerebrovascular diseases [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Production of a Series of Long-Chain Isomaltooligosaccharides from Maltose by Bacillus subtilis AP-1 and Associated Prebiotic Properties

Tiangpook

Nhim

Prangthip

et al. 2023

Foods

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Bacillus subtilis strain AP-1, which produces α-glucosidase with transglucosidase activity, was used to produce a series of long-chain isomaltooligosaccharides (IMOs) with degree of polymerization (DP) ranging from 2 to 14 by direct fermentation of maltose. A total IMOs yield of 36.33 g/L without contabacillusmination from glucose and maltose was achieved at 36 h of cultivation using 50 g/L of maltose, with a yield of 72.7%. IMOs were purified by size exclusion chromatography with a Superdex 30 Increase column. The molecular mass and DP of IMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Subsequently, linkages in produced oligosaccharides were verified by enzymatic hydrolysis with α-amylase and oligo-α-1,6-glucosidase. These IMOs showed prebiotic properties, namely tolerance to acidic conditions and digestive enzymes of the gastrointestinal tract, stimulation of probiotic bacteria growth to produce short-chain fatty acids and no stimulating effect on pathogenic bacteria growth. Moreover, these IMOs were not toxic to mammalian cells at up to 5 mg/mL, indicating their biocompatibility. Therefore, this research demonstrated a simple and economical method for producing IMOs with DP2–14 without additional operations; moreover, the excellent prebiotic properties of the IMOs offer great prospects for their application in functional foods.

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