The bHLH transcription factor regulated gene OsWIH2 is a positive regulator of drought tolerance in rice

Gu, Xiangyang; Gao, Shuxin; Li, Jing; Song, Pengyu; Zhang, Qian; Guo, Jianrong; Wang, Xiaoyan; Han, Xiaoyu; Wang, Xiaoji; Zhu, Ying; Zhang, Zhu

doi:10.1016/j.plaphy.2021.11.031

Cited by 21 publications

(14 citation statements)

References 54 publications

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“…The osbhlh024 mutant (A91), with a nucleotide base deletion generated by the CRISPR/Cas9 strategy, increased the shoot weight, and produced high antioxidant activities under salt stress, which indicates that OsbHLH024 might play a negative role in the salt stress response of rice [ 136 ]. In addition, OsWIH2 , which is a drought induced WIH gene, was activated by OsbHLH130, and the overexpression of OsbHLH130 resulted in substantially higher drought tolerance via its participation in cuticular wax biosynthesis, with reductions in the water loss rate and ROS accumulation ( Figure 2 ) [ 137 ]. OsbHLH057 targeted the AATCA cis -element, in the promoter of Os2H16 , a gene responding to fungal attack in rice, and overexpression of OsbHLH057 enhanced rice disease resistance to fungus Rhizoctonia solani and drought tolerance [ 138 ].…”

Section: The Bhlh Transcription Factor Family In Plantsmentioning

confidence: 99%

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Zuo

Lee

Kang

2023

IJMS

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Plant basic helix-loop-helix (bHLH) transcription factors are involved in many physiological processes, and they play important roles in the abiotic stress responses. The literature related to genome sequences has increased, with genome-wide studies on the bHLH transcription factors in plants. Researchers have detailed the functionally characterized bHLH transcription factors from different aspects in the model plant Arabidopsis thaliana, such as iron homeostasis and abiotic stresses; however, other important economic crops, such as rice, have not been summarized and highlighted. The bHLH members in the same subfamily have similar functions; therefore, unraveling their regulatory mechanisms will help us to identify and understand the roles of some of the unknown bHLH transcription factors in the same subfamily. In this review, we summarize the available knowledge on functionally characterized bHLH transcription factors according to four categories: plant growth and development; metabolism synthesis; plant signaling, and abiotic stress responses. We also highlight the roles of the bHLH transcription factors in some economic crops, especially in rice, and discuss future research directions for possible genetic applications in crop breeding.

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Section: The Bhlh Transcription Factor Family In Plantsmentioning

confidence: 99%

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Zuo

Lee

Kang

2023

IJMS

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“…Similarly, NAC in Arabidopsis regulates xylem vessel differentiation, thereby affecting water conduction. Previous reports have shown that bHLH genes isolated from angiosperms enhance the drought tolerance of plants (Gu et al, 2021;Qian et al, 2021;Gao et al, 2022). Specifically, bHLH is an important component in the stomatal development of P. patens, and is, therefore, involved in the regulation of drought tolerance (Caine et al, 2020).…”

Section: Desiccation Tolerance Related Tfs In S Caninervismentioning

confidence: 99%

Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis

Yang

Li²,

Yang³

et al. 2023

Front. Plant Sci.

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Syntrichia caninervis is a desiccation tolerant moss and is the dominant bryophyte found in biological soil crusts in the Gurbantunggut desert. In this study, we assessed the transcriptome profiles of S. caninervis gametophytes during the dehydration-rehydration (D-R) process (across 9 time points) using Illumina sequencing. In total, 22489 transcripts were identified, including 5337 novel transcripts, that mapped to the reference genome. A total of 12548 transcripts exhibited significant alterations in the D-R samples compared with the control samples. The differentially expressed transcripts (DETs) possessed several enriched Gene Ontology terms, such as “water stress response”, “oxidation-reduction process”, “membrane metabolism”, “photosynthesis”, and “transcription factor activity”. Moreover, during early dehydration stress, the DETs were significantly enriched in stress-related pathways from the Kyoto Encyclopedia of Genes and Genomes, such as “phenylpropanoid biosynthesis”, “alpha-linolenic acid metabolism”, and “fructose and mannose metabolism”. Photosynthesis-related transcripts (e.g., ScPsa H, ScRubisco, and ScLhcb1) were inhibited during the dehydration treatment and significantly accumulated during the late rehydration period. Most transcripts from the late embryogenesis abundant proteins (LEA) and early light-inducible protein (ELIP) families strongly accumulated at the late dehydration stage. These pathways were positively correlated with the content changes of absolute water content and Fv/Fm values, alongside peroxidase and superoxide dismutase activities. Seven transcription factor families, including AP2-ERF, bHLH, G2-like, MYB, NAC, WRKY, and bZIP, were enriched in DETs during D-R treatment. This study is the first transcriptome analysis using the S. caninervis genome for gene annotation and multigroup D-R treatment points. Our results demonstrated the detailed dynamic changes in the transcriptome of S. caninervis during the D-R process. These results also improve understanding of desiccation tolerant plants’ adaptations to desiccation stress at the transcription level and provide promising gene resources for transgenic crop breeding.

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“…Moreover, the primary signal caused by drought is a water deficit, leading to osmotic stress. Under osmotic stress created by PEG treatment, the shoots and roots of OsWIH2 -overexpressing rice plants are longer than those of wild-type rice plants ( Gu et al., 2021 ). OsWIH2 -overexpressing rice plants have a significantly higher survival rate and a lower water loss rate compared with wild-type rice plants ( Gu et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Under osmotic stress created by PEG treatment, the shoots and roots of OsWIH2 -overexpressing rice plants are longer than those of wild-type rice plants ( Gu et al., 2021 ). OsWIH2 -overexpressing rice plants have a significantly higher survival rate and a lower water loss rate compared with wild-type rice plants ( Gu et al., 2021 ). Glutathione peroxidase 1 (GPX1) acts as a redox transducer and confers osmotic stress tolerance in rice by interacting with bZIP68 and promoting the oxidation of bZIP68 ( Zhou et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

OsWRKY76 positively regulates drought stress via OsbHLH148-mediated jasmonate signaling in rice

et al. 2023

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Drought stress is a major environmental threat that limits plant growth and crop productivity. Therefore, it is necessary to uncover the molecular mechanisms behind drought tolerance in crops. Here, OsWRKY76 positively regulated drought stress in rice. OsWRKY76 expression was induced by PEG treatment, dehydration stress, and exogenous MeJA rather than by no treatment. Notably, OsWRKY76 knockout weakened drought tolerance at the seedling stage and decreased MeJA sensitivity. OsJAZ12 was significantly induced by drought stress, and its expression was significantly higher in OsWRKY76-knockout mutants than in wild-type ZH11 under drought stress. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that OsWRKY76 interacted with OsJAZ12. OsWRKY76 weakened the interaction between OsbHLH148 and OsJAZ12 in yeast cells. The OsJAZ12 protein repressed the transactivation activity of OsbHLH148, and this repression was partly restored by OsWRKY76 in rice protoplasts. Moreover, OsDREB1E expression was lower in OsWRKY76-knockout mutants than in wild-type ZH11 under drought stress, but it was upregulated under normal growth conditions. Yeast one-hybrid, electrophoretic mobility shift, and dual-luciferase assays showed that OsWRKY76 and OsbHLH148 bound directly to the OsDREB1E promoter and activated OsDREB1E expression in response to drought stress. These results suggest that OsWRKY76 confers drought tolerance through OsbHLH148-mediated jasmonate signaling in rice, offering a new clue to uncover the mechanisms behind drought tolerance.

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The bHLH transcription factor regulated gene OsWIH2 is a positive regulator of drought tolerance in rice

Cited by 21 publications

References 54 publications

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert moss Syntrichia caninervis

OsWRKY76 positively regulates drought stress via OsbHLH148-mediated jasmonate signaling in rice

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