The beta-actin gene promoter of rohu carp (Labeo rohita) drives reporter gene expressions in transgenic rohu and various cell lines, including spermatogonial stem cells

Barman, Hirak Kumar; Mohanta, Ramya; Patra, Swagat Kumar; Chakrapani, Vemulawada; Panda, Rudra Prasanna; Nayak, S.K.; Jena, Sasmita; Jayasankar, P.; Nandanpawar, Priyanka

doi:10.1515/cmble-2015-0010

Cited by 11 publications

(10 citation statements)

References 10 publications

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“…In ICAR-CIFA, the group of scientists initiated to work on transgenic fishes and reported several papers with respect to the basic researches, gene therapy and transgenics (Barman et al 2010;Mohapatra et al 2010). The functional β-actin gene promoter of rohu carp, capable driving ubiquitous expression, was identified (Barman et al 2015). The isolated β-actin gene promoter/regulatory region are conserved among carps.…”

Section: National Status On Transgenic Fish Productionmentioning

confidence: 99%

“…This promoter can be used in any species of interests for foreign gene expressions (Hall et al 2007;Her et al 2004;Kinoshita et al 2000;Rembold et al 2006). Contrary to this, the isolated of the rohu (L. rohita) myosin light polypeptide chain 2 (mylz2) promoter (1.2 kb) could be used for expression in targeted skeletal muscle (Mohanta et al 2014;Barman et al 2015).…”

Section: National Status On Transgenic Fish Productionmentioning

confidence: 99%

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Status and prospects of gene editing and transgenic in fishes

Barman¹,

Rasal²,

Mondal³

2019

IJGPB

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Recent advances in high throughout DNA sequencing technologies have revolutionized for better understanding of structure-functional relationships of genes in identifying trait-associated transcriptomes and their regulated gene expressions. Subsequent breakthroughs in gene editing technologies such as zinc finger nucleases, transcriptional activator-like effect or nucleases (TALEN) and CRISPR (clustered regularly interspaced short palindromic repeats) determined chromosomal loci so as to understand gene functions in vivo. Such editing technologies are now being implemented in many laboratories due to an affordable cost and easiness of techniques. Targeted gene delivery and disruptions are now not only restricted to standard cell lines or stem cells, but also primary cell lines and nonmodel agriculturally important species. Progress and implications of gene integration and disruptions in food fishes like salmon, carps, etc. will be highlighted. The positive impacts on myostatin gene (negative growth hormone regulator) disruption mediated muscular growth have been documented. Transposon mediated gene integration technologies for value-additions to small indigenous aquarium fishes by expressing attractive fluorescent color genes could be the future of rainbow revolution. Issues linked with further-tuning with regards to improved efficacy and specificity, while reducing offtarget effects of gene editing tools will be addressed. There are health and environmental concerns with genetically modified organisms (GMOs). CRISPR/Cas9 mediated editing generates indels and hence supposed to be free from transgene-nontoxic and non-allergen. Scientific progress regarding to generate genetically modified carps; those could well be cultivated in a confinement and at the same time economically profitable; will be highlighted. Emphasis should be given for transfer these technologies from the laboratory to land for the development of a consumer-friendly sustainable farming system.

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Section: National Status On Transgenic Fish Productionmentioning

confidence: 99%

Section: National Status On Transgenic Fish Productionmentioning

confidence: 99%

Status and prospects of gene editing and transgenic in fishes

Barman¹,

Rasal²,

Mondal³

2019

IJGPB

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“…The modulated gene expressions (mRNA levels) induced by hypoxic stress exposure were quantified by qPCR analysis using Light Cycler -480 SYBR Green I kit (Roche Diagnostics, Mannheim, Germany) in a Light Cycler 480 q -PCR instrument (Roche Diagnostics, Mannheim, Germany), as described elsewhere (Mohapatra et al, 2010; T GT GAGT GT CCT GT T CAAACGG 22 Barman, & Mohapatra, 2011;Barman et al, 2012;Barman et al, 2015;Patra et al, 2015). The mRNAs fro m the livers of ≥5 indiv iduals (fro m triplicate) were extracted independently fro m each day (39, 45, 47 and 61 days) of hypoxic and normo xic treat ments.…”

Section: Quantification Of Mrna Level By Quantitative Real Time Pcr (mentioning

confidence: 99%

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Mohapatra¹,

Chakrapani²,

Rasal³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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Channa striatus is a large-bodied fish of freshwater habitat capable of withstanding hypoxic conditions. Adaptive response to hypoxia is a complex physiological process. A laboratory -based rearing protocol for investigating long-term hypoxia stress tolerance in this species was established. Using suppression subtractive hybridization technique, we examined gene expression patterns in liver during prolonged hypoxia exposure. A total of 130 transcripts from the enriched cDNA library, under hypoxic condition, were sequenced. BLAST analysis identified 58%, 18% and 24% as known, uncharacterized and unknown sequences, respectively. All known genes represented a broad spectrum of biological pathways such as transcription/translation, signal transduction, electron transport, immune response, reproduction, cellular transportation. Heightened abundances for 11 Known, 1 uncharacterized and 1 unknown mRNA in the hypoxic liver were documented. Among these, the full-length cDNA sequences for heat shock protein 90 and CSHL-338 clone (uncharacterized) were generated using RACE strategy. Full-length cDNA sequences of prefoldin and fatty acid binding protein was obtained from the respective clones of SSH cDNA library, were also up -regulated during hypoxia stress. In this study, possible physiological significances about hypoxia-tolerance transcripts have been discussed. The ESTs presented here will have potential future implications in exploring new mechanisms of hypoxia acclimation and/or tolerance in C. striatus.

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“…Beta-actin is a housekeeping gene with ubiquitous expression. The promoter of beta-actin gene has been isolated from and characterized in a wide variety of species such as human ( Gunning et al, 1987 ), chicken ( Kosuga et al, 2000 ), amphioxus ( Feng et al, 2014 ), teleosts ( Barman et al, 2015 ; Hwang et al, 2003 ; Kong et al, 2014 ; Liu et al, 1991 ; Noh et al, 2003 ) and shrimps ( Shi et al, 2015 ; Shi et al, 2016 ). Because the beta-actin gene promoter confers a high level of constitutive transcriptional expression, it is widely used for transgenesis in both plants and animals ( Cho et al, 2011 ; Hong et al, 2016 ; Kosuga et al, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii)beta-actingene promoter

Yan

Gao

Cui

et al. 2018

PeerJ

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Constitutive promoters are important tools for gene function studies and transgenesis. The Beta-actin (actb1) gene promoter has been isolated from many species but remains to be cloned from the giant freshwater prawn (Macrobrachium rosenbergii). In this study, we cloned and characterized the Mractb1 gene promoter. Two alternative promoters were identified for the Mractb1 gene, which direct the generation of two transcripts with different 5′ untranslated regions. Three CpG islands were predicted in the upstream sequence, which are intimately related to transcription initiation and promoter activity. In addition to the CCAAT-box and the CArG-box, molecular dissection of the flanking sequence revealed the existence of one negative and two positive elements in the upstream region and the first intron. Finally, the Mractb1 promoter demonstrated comparative activity to the carp (Cyprinus carpio) actb1 promoter. Our investigations provide a valuable genetic tool for gene function studies and shed light on the regulation of the Mractb1 gene.

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The beta-actin gene promoter of rohu carp (Labeo rohita) drives reporter gene expressions in transgenic rohu and various cell lines, including spermatogonial stem cells

Cited by 11 publications

References 10 publications

Status and prospects of gene editing and transgenic in fishes

Status and prospects of gene editing and transgenic in fishes

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Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii)beta-actingene promoter

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