The BES1/BZR1-family transcription factor MpBES1 regulates cell division and differentiation in Marchantia polymorpha

Mecchia, Martín A.; García-Hourquet, Mariano; Lozano-Elena, Fidel; Planas-Riverola, Ainoa; Blasco‐Escámez, David; Marquès-Bueno, Mar; Mora-Garcı́a, Santiago; Caño‐Delgado, Ana I.

doi:10.1016/j.cub.2021.08.050

Cited by 21 publications

(18 citation statements)

References 75 publications

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“…Due to the complexity of fern genomes and the immaturity of transformation technology, it remains challenging to create mutants. Recently, it was reported that when the BZR1/BES1 family gene of Marchantia polymorpha was knocked out, the growth was significantly reduced, indicating that the BZR1/BES1 component plays an important role in the growth and development of Marchantia polymorpha [ 47 ]. Based on this, we speculate that BR signaling in ferns plays an important role and that their specific biological functions need to be further explored.…”

Section: Discussionmentioning

confidence: 99%

Evolutionary Analysis and Functional Identification of Ancient Brassinosteroid Receptors in Ceratopteris richardii

Zheng

Xing

Zhang

et al. 2022

IJMS

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Phytohormones play an important role in the adaptive evolution of terrestrial plants. Brassinosteroids (BRs) are essential hormones that regulate multiple aspects of plant growth and development in angiosperms, but the presence of BR signaling in non-seed plants such as ferns remains unknown. Here, we found that BR promotes the growth of Ceratopteris richardii, while the synthetic inhibitor PCZ inhibits the growth. Using full-length transcriptome sequencing, we identified four BRI1-like receptors. By constructing chimeric receptors, we found that the kinase domains of these four receptors could trigger BR downstream signaling. Further, the extracellular domains of two receptors were functionally interchangeable with that of BRI1. In addition, we identified a co-receptor, CtSERK1, that could phosphorylate with CtBRL2s in vitro. Together, these proved the presence of a receptor complex in Ceratopteris richardii that might perceive BR and activate downstream hormone signaling. Our results shed light on the biological and molecular mechanisms of BR signaling in ferns and the role of BR hormone signaling in the adaptive evolution of terrestrial plants.

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Section: Discussionmentioning

confidence: 99%

Evolutionary Analysis and Functional Identification of Ancient Brassinosteroid Receptors in Ceratopteris richardii

Zheng

Xing

Zhang

et al. 2022

IJMS

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“…MpBES1 phosphorylation by MpGSK was much weaker than the phosphorylation of BES1/BZR1 by BIN2 (Mecchia et al 2021). Moreover, the interaction between MpBES1 and MpGSK was not stronger as that between BES1 and BIN2 or MpGSK in yeast two-hybrid system (Mecchia et al 2021). However, the phenotypes of both Mpbes1 and Mpgsk show same trend in terms of repression of the cell differentiation, suggesting that MpBES1 is one of possible candidates for substrate of MpGSK in M. polymorpha.…”

Section: Mpgsk Regulates the Cell Proliferation And Differentiation F...mentioning

confidence: 96%

“…A single GSK3-like kinase, MpGSK (Mp7g04170; Supplementary Figure S2A), was identified in the genome of M. polymorpha (MarpolBase genome database [v5.1: http://marchantia. info]; Mecchia et al 2021;Montgomery et al 2020). MpGSK has a highly conserved serine/threonine protein kinase domain including the activation loop and the TREE domain for the proteasome-mediated protein degradation (Peng et al 2008), as other plant GSK3s possess (Supplementary Figure S2B).…”

Section: Polymorpha Has a Single Gsk3-like Kinase Orthologmentioning

confidence: 99%

“…Transcription factors BES1 and its homolog BZR1 are well-known substrates of GSK3s such as BIN2 in A. thaliana (He et al 2002;Kondo et al 2014;Yin et al 2002). Recently, it was reported that the ortholog of A. thaliana BES1 and BZR1, MpBES1, is involved in the regulation of cell proliferation and cell differentiation in M. polymorpha (Mecchia et al 2021). MpBES1 phosphorylation by MpGSK was much weaker than the phosphorylation of BES1/BZR1 by BIN2 (Mecchia et al 2021).…”

Section: Mpgsk Regulates the Cell Proliferation And Differentiation F...mentioning

confidence: 99%

“…Recently, it was reported that the ortholog of A. thaliana BES1 and BZR1, MpBES1, is involved in the regulation of cell proliferation and cell differentiation in M. polymorpha (Mecchia et al 2021). MpBES1 phosphorylation by MpGSK was much weaker than the phosphorylation of BES1/BZR1 by BIN2 (Mecchia et al 2021). Moreover, the interaction between MpBES1 and MpGSK was not stronger as that between BES1 and BIN2 or MpGSK in yeast two-hybrid system (Mecchia et al 2021).…”

Section: Mpgsk Regulates the Cell Proliferation And Differentiation F...mentioning

confidence: 99%

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A glycogen synthase kinase 3-like kinase MpGSK regulates cell differentiation in <i>Marchantia polymorpha</i>

Furuya

Nishihama

Ishizaki

et al. 2022

Plant Biotechnology

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Plants precisely coordinate the balance between cell proliferation and differentiation to ensure the continuous development. In Arabidopsis thaliana, members of glycogen synthase kinase 3 (GSK3) family, which are highly conserved serine/threonine protein kinases among eukaryotes, play important roles in regulating cell proliferation and differentiation during various developmental processes. However, functional roles of GSK3s in the plant lineages except angiosperms remain to be elucidated. Here, we utilized a model liverwort, Marchantia polymorpha, for studies of GSK3, because it has a single GSK3-like kinase, MpGSK. When M. polymorpha was treated with a chemical compound, bikinin, which is known as a specific inhibitor for GSK3-like kinases, growth and morphologies were altered with an expansion of the meristematic region. Similarly, Mpgsk loss-of-function mutants accumulated undifferentiated cell mass with no differentiated tissues. By contrast, overexpression of MpGSK reduced the size of the meristem region. These results suggest that MpGSK plays important roles as a regulator for the balance between cell differentiation and proliferation in M. polymorpha.

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A conserved brassinosteroid-mediated BES1-CERP-EXPA3 signaling cascade controls plant cell elongation

Zhu¹,

Wang²,

Zhu³

et al. 2023

Cell Reports

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The BES1/BZR1-family transcription factor MpBES1 regulates cell division and differentiation in Marchantia polymorpha

Cited by 21 publications

References 75 publications

Evolutionary Analysis and Functional Identification of Ancient Brassinosteroid Receptors in Ceratopteris richardii

Evolutionary Analysis and Functional Identification of Ancient Brassinosteroid Receptors in Ceratopteris richardii

A glycogen synthase kinase 3-like kinase MpGSK regulates cell differentiation in <i>Marchantia polymorpha</i>

A conserved brassinosteroid-mediated BES1-CERP-EXPA3 signaling cascade controls plant cell elongation

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