The Benefits of Active Compounds in Kalanchoe Pinnata (Lmk) Pers Ethyl Acetate Fraction on Lupus Arthritis Mice

Abstract

Objective: The aim of this research was to measure the effects of the ethyl acetate fraction of Kalanchoe pinnata (Lmk) Pers (EF-KP) on lupus arthritis mice. Methods:The research was performed by testing of CD123 + interferon-α (IFN-α + ) dendritic cells and CD68 + interleukin 6 (IL-6 + ) macrophages as the main biomarkers using flow cytometry method, and then, the outcomes were directly observed in the joint's tissue structure. The results of the research were analyzed using statistics. Results IL-6+ macrophages but not significantly. Finally, the outcome to the grade of joint damage was scored using Pritzker method. The treated groups have one grade lower, and the joint spaces were narrower than the untreated group. Conclusion:The results show the ability of the active compounds in EF-KP, which are comparable to 0.042 mg/kg of quercetin, to inhibit the progress of lupus arthritis pathogenesis in mice. It might reveals the effectiveness of the EF-KP in human with lupus arthritis. However, the further clinical research is necessary.

Repurposing of Bryophyllum pinnatum for dysmenorrhea treatment: a systematic scoping review and case series

“…Some identified publications focused on the immunomodulatory effect of B. pinnatum extracts and compounds. In lupus mice, B. pinnatum reduced T cell activation ( Indriyanti et al, 2018b ) and B cell maturation, decreased production of TNF-α ( Dantara et al, 2021 ) and improved histopathological nephritis markers ( Indriyanti et al, 2018a ). In addition, the aqueous extract and the flavonoid quercetin reduced in vitro mast cell activation and in vivo airway inflammation in allergic airway models ( Cruz et al, 2012 ).…”

Exploring the therapeutic mechanism of potential phytocompounds from Kalanchoe pinnata in the treatment of diabetes mellitus by integrating network pharmacology, molecular docking and simulation approach

“…Through topological research, the essential pharmaceuticals for the treatment of diabetes derived from K. pinnata were determined. Furthermore, the authors have verified the existence of diverse bioactive substances in the leaf extract of this plant, particularly abundant in quercetin, a flavonoid known for its remarkable efficacy in reducing lupus arthritis in a mouse model ( Indriyanti et al, 2017 ). As per the findings of ( Ogidigo et al, 2022 ) ethyl acetate fractions of K. Pinnata contains significant amounts of quercetin, luteolin, isorhamnetin, and luteolin-7-glucoside, the extract exhibited a reduction in an oxidative imbalance in rats subjected to neurotoxicity induced by aluminium chloride; these results indicate a potential neuroprotective effect.…”

Bioactive Compounds from Kalanchoe Genus Potentially Useful for the Development of New Drugs

“…Due to the importance of identifying the bioactive compound, the number of papers tentatively attributing the biological activity of Kalanchoe extracts to flavonoids or to flavonoid-enriched fractions is increasing nowadays. In a study on the activity of K. pinnata in a mice model of lupus arthritis, the authors observed that the ethyl acetate fraction (rich in quercetin glycosylated flavonoids), obtained from a leaf aqueous extract, inhibited the progression of the disease [ 54 ]. This fraction also produced positive effects on anti-Smith antibody and T reg in lupus mice [ 55 ].…”

“…In a study on the activity of K. pinnata in a mice model of lupus arthritis, the authors observed that the ethyl acetate fraction (rich in quercetin glycosylated flavonoids), obtained from a leaf aqueous extract, inhibited the progression of the disease [ 54 ]. This fraction also produced positive effects on anti-Smith antibody and T reg in lupus mice [ 55 ]. Still regarding K. pinnata , ethyl acetate fractions enriched in quercetin, luteolin, isorhamnetin, and luteolin-7-glucoside ameliorated oxidative imbalance in neurotoxicity caused by aluminum chloride in rats, showing a neuroprotective effect [ 56 ].…”