Objectives: Expedient extubation following cardiac surgery has been associated with improved outcomes, leading to postoperative extubation frequently during overnight hours. However, recent evidence in a mixed medical-surgical intensive care unit population associated overnight extubation with worse outcomes. This study investigated the impact of overnight extubation in a statewide, multicenter Society of Thoracic Surgeons (STS) database. Methods: Records from 39,812 patients undergoing coronary artery bypass grafting and/ or valve operations (2008-2016) and extubated within 24 hours were stratified according to extubation time between 06:00-18:00 (day) or 18:00-6:00 (overnight). Outcomes including reintubation, mortality, and composite morbidity-mortality were evaluated using hierarchical regression models adjusted for STS predictive risk scores. To further analyze extubation during the night, a subanalysis stratified patients into three groups