The benefits and limitations of reaction cell and sector field inductively coupled plasma mass spectrometry in the detection and quantification of phosphopeptides

Abstract

This one-to-one comparison of two interference-reducing ICP-MS instruments demonstrates that absolute quantification of individual LC-separated phosphopeptides is possible. However, based on the LOD values, SF-ICP-MS has a higher sensitivity in detecting phosphorus signals and thus is preferred in phosphopeptide analysis.

Ultratrace Metal Speciation Analysis by Coupling of Sector-Field ICP-MS to High-Resolution Size Exclusion and Reversed-Phase Liquid Chromatography

“…These features were already exploited for detection of phosphorus in small amounts of protein samples and for detection of metal complexes in rat liver . A direct comparison of sector-field ICP-MS and quadrupole ICP-MS with reaction cell showed that the interference removal with sector-field ICP-MS is more efficient for biological samples. , However, all of these previous studies suffered from the fact that increasing mass resolution causes loss of sensitivity - already the switch from low ( m / z = 300) to medium ( m / z = 4000) mass resolution causes an about 10× lowering of signal intensity. In all examples shown in the current study, medium resolution mode was used, which resolved all interferences on all elements shown here.…”

“…The most significant single enhancement may be the development of a double focusing optics, leading to so-called sector-field ICP-MS. This has been shown to greatly enhance sensitivity, compared to conventional quadropole ICP-MS, while eliminating most matrix interferences. , Triple quadrupole ICP-MS improves the removal of matrix interferences compared to conventional single-quadrupole instruments as well, but a direct comparison to sector-field ICP-MS for biological samples was not yet published. Further sensitivity was gained in all ICP-MS types by replacing the standard spray chamber with a desolvating nebulizer (Apex Q, Elemental Scientific, Omaha, NE, USA).…”

Establishment of measurement traceability for peptide and protein quantification through rigorous purity assessment—a review

“…Se) [158][159][160][161] or non-metal (e.g. P) [162][163][164][165][166][167][168] and on elements added through derivatisation (e.g. I, Cu Eu, Lu) [169][170][171][172][173][174].…”

Strategies to overcome spectral interference in ICP-MS detection

“…In this case, O 2 and O 2 /He have been adopted to convert the interfering species into PO + and SO + species. [45][46][47] In one study for P + detection, He was found to provide a better signal-to-noise ratio than using H 2 as the collision gas. 48 Mn is another example of a monoisotopic element which is impossible to detect among alternative isotopes; at the same time, it suffers interference from the Ar-, N-, and O-containing species.…”

“…For example, sector-eld ICP-MS (SF-ICP-MS) operating at a resolution of 4000 can resolve the spectral interference for phosphorus determination. 46 However, one drawback of HR-ICP-MS is that, due to the requirements of high mass resolution analysis, the ion transmission efficiency is greatly diminished (e.g. 10-fold reduction).…”